Melatonin Receptor 1A (MTNR1A) (NM_005958) Human Untagged Clone

CAT#: SC116399

MTNR1A (untagged)-Human melatonin receptor 1A (MTNR1A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Melatonin Receptor 1A
• Synonyms: MEL-1A-R; MT1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005958, RT-PCR generated
  ATGCAGGGCAACGGCAGCGCGCTGCCCAACGCCTCCCAGCCCGTGCTCCGCGGGGACGGC
  GCGCGGCCCTCGTGGCTGGCGTCCGCCCTGGCCTGCGTCCTCATCTTCACCATCGTGGTG
  GACATCCTGGGCAACCTCCTGGTCATCCTGTCGGTGTATCGGAACAAGAAGCTCAGGAAC
  GCAGGAAACATCTTTGTGGTGAGCTTAGCGGTGGCAGACCTGGTGGTGGCCATTTATCCG
  TACCCGTTGGTGCTGATGTCGATATTTAACAACGGGTGGAACCTGGGCTATCTGCACTGC
  CAAGTCAGTGGGTTCCTGATGGGCCTGAGCGTCATCGGCTCCATATTCAACATCACCGGC
  ATCGCCATCAACCGCTACTGCTACATCTGCCACAGTCTCAAGTACGACAAACTGTACAGC
  AGCAAGAACTCCCTCTGCTACGTGCTCCTCATATGGCTCCTGACGCTGGCGGCCGTCCTG
  CCCAACCTCCGTGCAGGGACTCTCCAGTACGACCCGAGGATCTACTCGTGCACCTTCGCC
  CAGTCCGTCAGCTCCGCCTACACCATCGCCGTGGTGGTTTTCCACTTCCTCGTCCCCATG
  ATCATAGTCATCTTCTGTTACCTGAGAATATGGATCCTGGTTCTCCAGGTCAGACAGAGG
  GTGAAACCTGACCGCAAACCCAAACTGAAACCACAGGACTTCAGGAATTTTGTCACCATG
  TTTGTGGTTTTTGTCCTTTTTGCCATTTGCTGGGCTCCTCTGAACTTCATTGGCCTGGCC
  GTGGCCTCTGACCCCGCCAGCATGGTGCCTAGGATCCCAGAGTGGCTGTTTGTGGCCAGT
  TACTACATGGCGTATTTCAACAGCTGCTTCAATGCCATTATATACGGGCTACTGAACCAA
  AATTTCAGGAAGGAATACAGGAGAATTATAGTCTCGCTCTGTACAGCCAGGGTGTTCTTT
  GTGGACAGCTCTAACGACGTGGCCGATAGGGTTAAATGGAAACCGTCTCCACTGATGACC
  AACAATAATGTAGTAAAGGTGGACTCCGTTTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005958 unedited
  GGCCGAGGTCAAGATTTTGTATACGACTCACTATAGGGCGGCCGCCAGTGTGATGGAATC
  TGCAGAATTCGGCTTGACGCGAACAGGGACCATGCAGGGCAACGGCAGCGCGCTGCCCAA
  CGCCTCCCAGCCCGTGCTCCGCGGGGACGGCGCGCGGCCCTCGTGGCTGGCGTCCGCCCT
  GGCCTGCGTCCTCATCTTCACCATCGTGGTGGACATCCTGGGCAACCTCCTGGTCATCCT
  GTCGGTGTATCGGAACAAGAAGCTCAGGAACGCAGGAAACATCTTTGTGGTGAGCTTAGC
  GGTGGCAGACCTGGTGGTGGCCATTTATCCGTACCCGTTGGTGCTGATGTCGATATTTAA
  CAACGGGTGGAACCTGGGCTATCTGCACTGCCAAGTCAGTGGGTTCCTGATGGGCCTGAG
  CGTCATCGGCTCCATATTCAACATCACCGGCATCGCCATCAACCGCTACTGCTACATCTG
  CCACAGTCTCAAGTACGACAAACTGTACAGCAGCAAGAACTCCCTCTGCTACGTGCTCCT
  CATATGGCTCCTGACGCTGGCGGCCGTCCTGCCCAACCTCCGTGCAGGGACTCTCCAGTA
  CGACCCGAGGATCTACTCGTGCACCTTCGCCCAGTCCGTCAGCTCCGCCTACACCATCGC
  CGTGGGTGGTTTTCCACTTCCTCGTCCCCATGATCATAGTCATCTTCTGTTACCTGAGAA
  TATGGATCCTGGTTCTCCAGGTCAGACAGAGGGTGAAACCTGACCGCANACCCAAACTGA
  NACCACAGGACTTCAGGAATTTTGTCCCATGTTTGTGGGGTTTTGTCCTTTTTGCCATTT
  GCTGGGCTCCTCTGAACTCATTGGCCTGGCCGTGGCCTCTGACCCCGCAGCATGTGCCCT
  AGATCCCAGATGGCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005958 unedited
  GGGGACTAGGAATGGAAACTTCCATGCCAGNAAAGCACTGGGGNAGGGTCACAGGGCTGC
  CACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGACAAGCT
  TGATATCGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCT
  TTTAAACGGAGTCCACCTTTACTACATTATTGTTGGTCATCAGTGGAGACGGTTTCCATT
  TAACCCTATCGGCCACGTCGTTAGAGCTGTCCACAAAGAACACCCTGGCTGTACAGAGCG
  AGACTATAATTCTCCTGTATTCCTTCCTGAAATTTTGGTTCAGTAGCCCGTATATAATGG
  CATTGAAGCAGCTGTTGAAATACGCCATGTAGTAACTGGCCACAAACAGCCACTCTGGGA
  TCCTAGGCACCATGCTGGCGGGGTCAGAGGCCACGGCCAGGCCAATGAAGTTCAGAGGAG
  CCCAGCAAATGGCAAAAAGGACAAAAACCACAAACATGGTGACCAAATTCCTGAAGTCCT
  GTGGTTTCAGTTTGGGTTTGCGGTCAGGTTTCACCCTCTGTCTGACCTGGAGAACCAGGA
  TCCATATTCTCAGGTAACAAGAGATGACTATGATCATGGGGACGAGGAAGTGGAAAACCA
  CCCCGCGATGGTGTAAGCGGACCTGACNGACTGGGCGAAAGTGCACAAATAAATCCTCGG
  GTCCGACTGGGAGAGTCCCTGCACGGGAGGTTGGGCCAGGACGGCCCGCCACCGTCAGGA
  GCCCTTATGAAGAGCACGTACCAAAAGGAGTTCCTTGCTGCTGGCAAGTTTTGGTCGACT
  TGAAAATGGGGCAAAGTATAAATAGCGGG
  
• Restriction Sites: Please inquire
• ACCN: NM_005958
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005958.3, NP_005949.1
• RefSeq Size: 1105 bp
• RefSeq ORF: 1053 bp
• Locus ID: 4543
• UniProt ID: P48039
• Cytogenetics: 4q35.2
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin. [provided by RefSeq, Jul 2008]