TLE4 (NM_007005) Human Untagged Clone

CAT#: SC115738

TLE4 (untagged)-Human transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) (TLE4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TLE4
• Synonyms: BCE-1; BCE1; E(spI); E(spl); ESG; ESG4; Grg-4; GRG4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_007005, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATTCGCGACCTGAGCAAGATGTACCCGCAGACCAGACACCCGGCACCGCATCAGCCTGCTCAACCCT
  TTAAATTTACAATTTCCGAATCCTGTGATCGGATTAAGGAAGAGTTTCAGTTTTTACAGGCTCAATACCA
  CAGTCTGAAGCTGGAATGTGAGAAACTCGCCAGTGAGAAGACAGAGATGCAGCGGCATTATGTCATGTAT
  TATGAAATGTCCTATGGGTTGAATATAGAAATGCACAAGCAGGCAGAGATTGTCAAGAGGCTGAATGCTA
  TCTGTGCACAAGTCATTCCTTTCCTGTCCCAAGAGCACCAGCAACAAGTGGTGCAGGCTGTGGAACGGGC
  CAAGCAGGTGACCATGGCAGAACTGAACGCCATCATTGGGCAACAACTCCAGGCCCAGCATTTATCACAT
  GGACATGGTCTCCCCGTACCTCTGACTCCACACCCTTCAGGGCTCCAGCCCCCTGCCATTCCACCCATCG
  GTAGCAGTGCCGGGCTTCTGGCCCTCTCCAGTGCTCTAGGAGGTCAGTCCCATCTTCCAATTAAAGATGA
  GAAGAAGCACCATGACAATGATCACCAAAGAGACAGAGACTCCATCAAGAGCTCTTCAGTATCCCCATCA
  GCCAGTTTCCGAGGTGCTGAGAAGCACAGAAACTCCGCAGACTACTCCTCAGAGAGCAAAAAGCAGAAAA
  CTGAAGAAAAGGAAATTGCAGCTCGTTATGACAGCGATGGTGAGAAAAGTGATGACAACTTGGTGGTTGA
  CGTTTCCAATGAGGATCCATCTTCCCCTCGAGGGAGCCCAGCACATTCCCCCAGAGAGAATGGCCTAGAC
  AAGACACGCCTGCTCAAGAAAGATGCCCCGATTAGTCCAGCCTCTATTGCATCTTCCAGCAGTACTCCCT
  CCTCCAAATCCAAAGAACTTAGCCTTAATGAAAAATCTACTACTCCCGTCTCAAAGTCCAATACCCCTAC
  TCCACGAACTGATGCGCCCACCCCAGGCAGTAACTCTACTCCCGGATTGAGGCCTGTACCTGGAAAACCA
  CCAGGAGTTGACCCTTTGGCCTCAAGCCTAAGGACCCCAATGGCAGTACCTTGTCCATATCCAACTCCAT
  TTGGGATTGTGCCCCATGCTGGAATGAACGGAGAGCTGACCAGCCCCGGAGCGGCCTACGCTGGGCTCCA
  CAACATCTCCCCTCAGATGAGCGCAGCTGCTGCCGCCGCCGCTGCTGCTGCTGCCTATGGGAGATCACCA
  GTGGTGGGATTTGATCCACACCATCACATGCGTGTGCCAGCAATACCTCCAAACCTGACAGGCATTCCAG
  GAGGAAAACCAGCATACTCCTTCCATGTTAGCGCAGATGGTCAGATGCAGCCTGTCCCTTTTCCACCCGA
  CGCCCTCATCGGACCTGGAATCCCCCGGCATGCTCGCCAGATCAACACCCTCAACCACGGGGAGGTGGTG
  TGCGCGGTGACCATCAGCAACCCCACGAGACACGTGTACACGGGTGGGAAGGGCTGCGTCAAGGTCTGGG
  ACATCAGCCACCCAGGCAATAAGAGTCCTGTCTCCCAGCTCGACTGTCTGAACAGGGATAACTACATCCG
  TTCCTGCAGATTGCTCCCTGATGGTCGCACCCTAATTGTTGGAGGGGAAGCCAGTACTTTGTCCATTTGG
  GACCTGGCGGCTCCAACCCCACGCATCAAGGCAGAGCTGACATCCTCGGCCCCCGCCTGCTATGCCCTGG
  CCATCAGCCCCGATTCCAAGGTCTGCTTCTCATGCTGCAGCGACGGCAACATCGCTGTGTGGGATCTGCA
  CAACCAGACCTTGGTGAGGCAATTCCAGGGCCACACAGATGGAGCCAGCTGTATTGACATTTCTAATGAT
  GGCACCAAGCTCTGGACAGGTGGTTTGGACAACACGGTCAGGTCCTGGGACCTGCGCGAGGGGCGGCAGC
  TGCAGCAGCACGACTTCACCTCCCAGATCTTTTCTCTGGGCTACTGCCCAACTGGAGAGTGGCTTGCAGT
  GGGGATGGAGAACAGCAATGTGGAAGTTTTGCATGTCACCAAGCCAGACAAATACCAACTACATCTTCAT
  GAGAGCTGTGTGCTGTCGCTCAAGTTTGCCCATTGTGGCAAATGGTTTGTAAGCACTGGAAAGGACAACC
  TTCTGAATGCCTGGAGAACACCTTATGGGGCCAGTATATTCCAGTCCAAAGAATCCTCATCGGTGCTTAG
  CTGTGACATCTCCGTGGACGACAAATACATTGTCACTGGCTCTGGGGATAAGAAGGCCACAGTTTATGAA
  GTTATTTATTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007005 unedited
  NGGTGTTCAAATTTTCGTATACGACTCACTATAGGCGGCCGCGAATCGGCACGAGGCCGC
  ATCTAAAACCAAGGATTTCAGATACTCATTGCAGTTGTGGAGTTTAATCCCCTTTCTTAA
  CCTCACTTCCCACTTGCTATTGAATTGTGAATAGTCATTAAAAACCTGTGATACCAAATC
  TTCAGCTGTCTACTTGGAAGAACATGGAATAAGCATACTTAACAGTGAAAAGAATCTTTA
  ATTATGTATTATATCTGTAATATATTTATTTTGTTTAAAGAAGGCTTTCTAACAATGACT
  GACTAAATAAAGCTGTCTGCTCCTGCATTGATAATGAAGGTGCGTTGTATTTGATACCCC
  TCCCCCCCTTTTTTTGGCAAAGGAGGGGAAAGGAAGGTTTAAAATAATTGATTTAAAATG
  TCACTAAGTGTAGACTGATGACTGTATAGAGATGTGAAATGTATAATTACACATGGAAGC
  AATATGTTGCTGTGTTGTTATTAGGTTTTTTTTGTTTTTGTTTTCTACATCTTTTAAAGA
  CTTTTGGAAATTTGGCTGAACAATTAGAACACAACAGGCCAACTCATACTCATTTGGATC
  TATTTAGACAACGTTAACCAATATATCTATAGCTTTAGATTATATTCGATAAAAGTAATT
  GGACTTTTTTTCTTTTTTTGACTCATTGACAAGTGTCTTTGTAATATGTTTTTAGTTCCC
  TTTTTTTGTTGTATTATAGGCAGATGAACAAATTAAATTTGGCCTCAAAGAGAGAACTTA
  CTCCCTTCTGNATATTTTTGCCACATTTCTTTGCAAAAGGAGAATATATATCTTTAGTCA
  GTTNTGNTGNTATGAGAAACTATGGGTTATTTTGTGGCATGCTCTTTTGGAGCTGCACAG
  T
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007005 unedited
  GACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGAAGTTATTTATGT
  TTAATGCTTAAAAGTCTGAATTCACAAACAATCTACCATTATAGAAGTACTGGTGGTCAA
  TACAATGCATTAGAACTATGTACAACGCACAGTTTAGTATCAAAATCTTTCTACACTGTA
  GAGTTTTACGAAACTGTTAATGACATCAAACACTAAGCACTTAAGACACCATTTTTTTTT
  TTTCTGCTACCACATTAGGAACGTCAATGGACAGTCCATTTCAACTTGCAGCATCCATCC
  ATTTCTAGTATGAAATTAAGTAATTTTCTACTTATACAATAAAGTATATCTACACGGTTC
  TTTTGATTTTGATCCATAGCAGCAAAGGCACTGTACATCAGCAGATCCACAGACTTAAAA
  GATTTTTAAAGCATTCAAACAAAAAATAATAAAAAAAGAAGTCACATATTATAGTTTAAC
  AGCAACAACAACAACATAAAAGATAACACAATGTTTCTGGCACAATGGCACAGCCTGTGT
  CCATTTCAGGGACATCCAACTAAGACATGGAAAGAACCGGAGGTAAAGAAAGCAAGTCCT
  CATCCCGGAGAGAGTCCATTCTTTGTGTTTTTCAGAGGGAGGGCTCTGCAACCCTCCTCT
  GCTGTCCTTGTTTGTAAAAGACTTACTTTGACAGAGTGGGGATTCCTCCCATAACTGGGC
  AGCTCCCAAAAACATGCCCAAAAATACCCCTTATTTTCTCATACAACAAAACTGCTTAAG
  AATTATTTTTTTTCCTTTTGCAAAAAAAGTTGGCAAAATTCCCAGAGGGGATTAATTTCT
  CTTTTTGAGGGCAAAATTAATTTGTTACACTGCCTTTTATAACACCAAAAAGGGGACTTA
  AAACTATTTCCAAGGCCCTTGTCAATGGATCAAAAGAAAAAAAGTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007005
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007005.1, NP_008936.1
• RefSeq Size: 5078 bp
• RefSeq ORF: 255 bp
• Locus ID: 7091
• UniProt ID: Q04727
• Cytogenetics: 9q21.31
• Protein Families: Transcription Factors
• Gene Summary: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon in the coding region compared to variant 1. It encodes isoform 3 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.