FAM50B (NM_012135) Human Untagged Clone

CAT#: SC115529

FAM50B (untagged)-Human family with sequence similarity 50, member B (FAM50B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FAM50B
• Synonyms: D6S2654E; X5L
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115529 sequence for NM_012135 edited (data generated by NextGen Sequencing)
  ATGGCGCAGTACAAGGGCACCATGCGCGAGGCAGGCCGTGCCATGCACCTCCTCAAGAAG
  CGCGAAAGGCAGCGGGAGCAGATGGAGGTGCTGAAGCAGCGCATCGCCGAGGAGACCATC
  CTCAAGTCGCAGGTGGACAAGAGGTTCTCGGCGCATTACGACGCCGTGGAGGCCGAGCTG
  AAGTCCAGCACGGTGGGCCTGGTGACCCTGAACGACATGAAGGCCCGGCAGGAGGCCCTG
  GTCAGGGAGCGCGAGCGGCAGCTGGCCAAGCGCCAGCACCTGGAGGAGCAGCGGCTGCAG
  CAGGAGCGGCAGCGGGAGCAGGAGCAGCGGCGCGAGCGCAAGCGTAAGATCTCCTGCCTG
  TCCTTTGCACTAGACGACCTCGATGACCAGGCCGACGCGGCCGAGGCCAGGCGCGCCGGA
  AACCTGGGCAAGAACCCCGACGTGGACACCAGCTTCCTGCCAGACCGCGACCGCGAGGAG
  GAGGAGAACCGGCTCCGAGAGGAGCTGCGCCAAGAGTGGGAGGCGCAGCGCGAGAAAGTG
  AAGGACGAGGAGATGGAGGTCACCTTCAGCTACTGGGACGGCTCGGGCCACCGGCGCACG
  GTGCGGGTGCGCAAGGGCAACACGGTGCAGCAGTTCCTGAAGAAGGCGCTGCAGGGGCTG
  CGCAAGGACTTCCTGGAGCTGCGCTCCGCCGGCGTGGAGCAGCTCATGTTCATCAAGGAG
  GACCTCATCCTGCCGCACTACCACACCTTCTACGACTTCATCATCGCCAGGGCGAGGGGC
  AAGAGCGGGCCGCTCTTCAGCTTCGATGTGCACGATGACGTGCGCCTGCTCAGCGACGCC
  ACCATGGAGAAGGACGAGTCGCACGCGGGCAAGGTGGTGCTGCGCAGCTGGTACGAGAAG
  AACAAGCACATCTTCCCCGCCAGCCGCTGGGAGGCCTATGACCCCGAGAAGAAGTGGGAC
  AAGTACACCATCCGCTAA
  
  Clone variation with respect to NM_012135.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012135 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGAGTGGGCCTCTGCT
  CGTGGGTGGTTCTCGTGGAGGTCAGCTCCCGCGTGTCTCCGCTCGACAGGGTGCTTGGGC
  AGAGCCCATCGGGTAGGCGCGGGCCATGGCGCAGTACAAGGGCACCATGCGCGAGGCAGG
  CCGTGCCATGCACCTCCTCAAGAAGCGCGAAAGGCAGCGGGAGCAGATGGAGGTGCTGAA
  GCAGCGCATCGCCGAGGAGACCATCCTCAAGTCGCAGGTGGACAAGAGGTTCTCGGCGCA
  TTACGACGCCGTGGAGGCCGAGCTGAAGTCCAGCACGGTGGGCCTGGTGACCCTGAACGA
  CATGAAGGCCCGGCAGGAGGCCCTGGTCAGGGAGCGCGAGCGGCAGCTGGCCAAGCGCCA
  GCACCTGGAGGAGCAGCGGCTGCAGCAGGAGCGGCAGCGGGAGCAGGAGCAGCGGCGCGA
  GCGCAAGCGTAAGATCTCCTGCCTGTCCTTTGCACTAGACGACCTCGATGACCAGGCCGA
  CGCGGCCGAGGCCAGGCGCGCCGGAAACCTGNGCAAGAACCCCGACGTGGACACCAGCTT
  CCTGCCAGACCGCGACCGCGAGGAGGAGGAGAAACCGCTCCGAGAGGAGCTGCGCCAAGA
  GTGGGAGGCGCAGCGCGAGAAAGTGAAGGACGAGGAGATGGAGGTCACCTTCAGCTACTG
  GGACGGCTCGGGCCACCGGCGCACGGTGCGGGTGCGCAAGGGCACACGGTGCANCAGNTC
  CTGAAAAAGGCGCTGCAGGNGCTGCGCAGGACCTCCTGGNAGCTGCGCTCGNCGGCGTGG
  AGCAGCTCATGTNCATCAAGGNAGACCTNATNCTGNCGNACTACACAACCTTTACGACTT
  ATNATCGNCAGGCGAGGGGCAAGAGCGGGCCGTCTTCACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012135 unedited
  CCGCAATCTAGAGTCGAGNNNTTNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA
  CAACAGAAACATTTATTAAAAGCTAAGCGTTTTACAAATATTAATTCACTTAATACTCAC
  ATCAATCTTATAAAGGAAGGGCTCTTGTTATTTCCTTTTACAGGGGACCAAACTGAGGCA
  TGGGGGTTTAATAAGCTTACCCAAAACTACACAGCAAGGAGGAGGCTGTGTCGGAAATCA
  AACTCAGTCACTGGGGCCCCCAAGTCCATGCTCATAACCACCAGGTAAAAAATGGGCCTC
  CTATTCTAATTTCTGTCTGACAGTGGGCCCAGTCATGGTTTCAATACAGCTCAGCAAAGC
  AATGCAGCAAATCCAGGTGGCAACACTAAAATACTCAAAAAAGACCAACCAAGCAACCAA
  AGCATAACATCAGCAATAGCACAACCAATGGGAATCATGAAAACCCTCTGTTTTTATTAA
  ATTGGGGGGAAAAAATCAAGGTCACAAAATCAAGTGACTGGGGATGGGGCCTAAAAATGA
  GTGTCTCCCCCCACCCCCGGTTTTCGTTCTGGCAGGCGGGTGTTAGCGGATGGTGTACTT
  GTCCCCACTCTTTTCGGGGTAATAGCCCCCCAGCGGTTGGCGGGAAAATGTGCCTTGTTT
  TTCTCGACCATTGCGCAACACCCACCTGCCCGGGTGCCATTCGCCTTTCTTCATGGTGGC
  GCCGTTGCACAAGCCCCCCTCCCCTGCCCCCTTAAACTAAGCAGCGGCCCCCTTTCCCCT
  CCCCCTGGCGAAAAAAACACCCAAAACGGGGGTAATGCCGTAATACACCGCCCTCTCTTA
  CAACCCACTTTCTCACCCGGGGACGCCCCCCAAAAATCTCTCCCACCCCGTAAGCGCTCC
  CACAAATCGCACCTGTTTTCTTGCCCAATACCGCCGCGGGCTATCTATCCAGCTGAATGC
  CATCCATCAACCCTCATCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012135
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012135.1, NP_036267.1
• RefSeq Size: 1618 bp
• RefSeq ORF: 978 bp
• Locus ID: 26240
• UniProt ID: Q9Y247
• Cytogenetics: 6p25.2
• Domains: XAP5
• Gene Summary: This gene contains an intronless ORF that arose from ancestral retroposition. The encoded protein is related to a plant protein that plays a role in the circadian clock. This gene is adjacent to a differentially methylated region (DMR) and is imprinted and paternally expressed in many tissues. [provided by RefSeq, Nov 2015]