AK2 (NM_013411) Human Untagged Clone

CAT#: SC115257

AK2 (untagged)-Human adenylate kinase 2 (AK2), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AK2
• Synonyms: ADK2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115257 sequence for NM_013411 edited (data generated by NextGen Sequencing)
  ATGGCTCCCAGCGTGCCAGCGGCAGAACCCGAGTATCCTAAAGGCATCCGGGCCGTGCTG
  CTGGGGCCTCCCGGGGCCGGTAAAGGGACCCAGGCACCCAGATTGGCTGAAAACTTCTGT
  GTCTGCCATTTAGCTACTGGGGACATGCTGAGGGCCATGGTGGCTTCTGGCTCAGAGCTA
  GGAAAAAAGCTGAAGGCAACTATGGATGCTGGGAAACTGGTGAGTGATGAAATGGTAGTG
  GAGCTCATTGAGAAGAATTTGGAGACCCCCTTGTGCAAAAATGGTTTTCTTCTGGATGGC
  TTCCCTCGGACTGTGAGGCAGGCAGAAATGCTCGATGACCTCATGGAGAAGAGGAAAGAG
  AAGCTTGATTCTGTGATTGAATTCAGCATCCCAGACTCTCTGCTGATCCGAAGAATCACA
  GGAAGGCTGATTCACCCCAAGAGTGGCCGTTCCTACCACGAGGAGTTCAACCCTCCAAAA
  GAGCCCATGAAAGATGACATCACCGGGGAACCCTTGATCCGTCGATCAGATGATAATGAA
  AAGGCCTTGAAAATCCGCCTGCAAGCCTACCACACTCAAACCACCCCACTCATAGAGTAC
  TACAGGAAACGGGGGATCCACTCCGCCATCGATGCATCCCAGACCCCCGATGTCGTGTTC
  GCAAGCATCCTAGCAGCCTTCTCCAAAGCCACATCCTAG
  
  Clone variation with respect to NM_013411.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013411 unedited
  GACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGG
  TGGCAGTGAGAGACTTCGGCGGACATGGCTCCCAGCGTGCCAGCGGCAGAACCCGAGTAT
  CCTAAAGGCATCCGGGCCGTGCTGCTGGGGCCTCCCGGGGCCGGTAAAGGGACCCAGGCA
  CCCAGATTGGCTGAAAACTTCTGTGTCTGCCATTTAGCTACTGGGGACATGCTGAGGGCC
  ATGGTGGCTTCTGGCTCAGAGCTAGGAAAAAAGCTGAAGGCAACTATGGATGCTGGGAAA
  CTGGTGAGTGATGAAATGGTAGTGGAGCTCATTGAGAAGAATTTGGAGACCCCCTTGTGC
  AAAAATGGTTTTCTTCTGGATGGCTTCCCTCGGACTGTGAGGCAGGCAGAAATGCTCGAT
  GACCTCATGGAGAAGAGGAAAGAGAAGCTTGATTCTGTGATTGAATTCAGCATCCCAGAC
  TCTCTGCTGATCCGAAGAATCACAGGAAGGCTGATTCACCCCAAGAGTGGCCGTTCCTAC
  CACGAGGAGTTCAACCCTCCAAAAGAGCCCATGAAAGATGACATCACCGGNGAACCCTTG
  ATCCGTCGATCAGATGATAATGAAAAGGCCTTGAAAATCCGNNCTGCAGCCTACCACACT
  CAAACCACCCCACTCATAGAGTACTACAGGAAACGGGGGATCCACTCCGNCATCGATGCA
  TCCCAGACCCCCGATGTCGTGTTCGCAAGCATCCTAGCAGCCTTCTCCAAGCCACATCCT
  AGTATCAGAGGGCCAGCGAGACTGCACACTGCTCATCACCCCGCGGCGTGATCCCTGCTC
  TTAGTGCTGGGCAAAGGGGAAGGNTGTTANGNTGAAGATGTGAGGNAGGNCTGNNTAGGG
  CTANNAGATACTNNGACACAGCAGTGTATTGTANTGTGCAGTTTCTTATACTAGGGGAGA
  GTTTTAAGTGTAGGAANAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013411 unedited
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTACAAATTTTAACAT
  ATTTTATTGATTTAGGGGCCAAACAAGGCAGCATTTGGTCAGTTAAGAAAGGCACCTCAT
  TGAAAATAATACATCACAGTGCTGTTGAGTGATCCCAGTCACAGGATTTGAGAATGGAAA
  GGACAATCTTTGGATGACATAGCTTAGGACACTTGCTCATTTATTTAAAAGAGTCCCTTC
  ACACAGTGCAGATAAAAGAAGGCAAGAGCATAGGATTGTGGCATGTGCTGGGTAGCTGTG
  AGAGGTTACTCATGAAAGTAGGTAGCCTAGTAAATTAGTGGTAAGTCAGGGGAAGGGAAT
  CAGCCTCCCTTTCCATTTCATTCCCTTCCCCCTCCCCTTGATTCCAGTTTGCTTGATTTG
  TTGAGCCACCATCCCTATGAATACACCTTACATCTCTTTCAAATGTGGAGATGGTTTTTT
  GATCTTACAGTGTGTGGAACCGGAGTCAGTAATCAAAGCTCCCTGTTCCAAGCTATAGAC
  AGACAGGACAACAATTTGATTTCCTGTACTCTTGGTCTGGAACCCATGGGGTTCTGAGAT
  AAAAGCTGAGGTCTTAGAGCACTGGCTTTAAAAAATATTTTCACCAGTCCAGAGTAAATG
  AAAACCCGATTCCTTAGNAAATATATTGGGTCTATTTCTACCCCCACCTCCAGCATGGTG
  TTTTTTAAAAATTAATTTTCCCTTACACTTTAAAACTTCACCTATGTATAAAAGAACTGC
  CACACTCAATAACACTGCTGTGGTTCCAATATTCCTTTGAACCCCTACACACCCTTCCTT
  AACTTTCTTACCTTGACACCCTTCCCTTTTGCCAAAACCTAAAACAGGAATCACCCGCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013411
• Insert Size: 1880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013411.2, NP_037543.1
• RefSeq Size: 2146 bp
• RefSeq ORF: 699 bp
• Locus ID: 204
• UniProt ID: P54819
• Cytogenetics: 1p35.1
• Domains: ADK, ADK_lid
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism
• Gene Summary: Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.[provided by RefSeq, Nov 2010]
  Transcript Variant: This variant (2), also known as AK2B, differs in the 3' UTR and coding sequence compared to variant 1. The encoded isoform (b) is shorter and has a distinct C-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.