VASH1 (NM_014909) Human Untagged Clone

CAT#: SC114754

VASH1 (untagged)-Human vasohibin 1 (VASH1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VASH1
• Synonyms: KIAA1036; TTCP 1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114754 sequence for NM_014909 edited (data generated by NextGen Sequencing)
  ATGCCAGGGGGGAAGAAGGTGGCTGGGGGTGGCAGCAGCGGTGCCACTCCAACGTCCGCT
  GCGGCCACCGCCCCCTCTGGGGTCAGGCGTTTGGAGACCAGCGAAGGAACCTCAGCCCAG
  AGAGATGAGGAGCCAGAAGAGGAAGGGGAAGAGGACCTGCGAGACGGAGGCGTCCCCTTC
  TTTGTCAACCGGGGTGGGCTACCTGTGGATGAGGCCACCTGGGAAAGGATGTGGAAACAC
  GTGGCCAAGATCCACCCCGATGGAGAGAAGGTGGCGCAACGGATCCGTGGGGCCACAGAC
  CTGCCCAAGATCCCCATACCGAGTGTGCCTACGTTCCAGCCGTCTACACCTGTCCCTGAG
  CGCCTGGAAGCTGTGCAGCGCTATATCAGAGAGCTGCAGTACAATCACACAGGGACACAG
  TTCTTTGAAATTAAGAAGAGCAGACCTCTGACAGGGCTGATGGACCTGGCCAAGGAAATG
  ACCAAAGAGGCCCTGCCAATCAAATGCCTGGAAGCCGTGATCCTGGGAATTTACCTCACC
  AACAGCATGCCCACCCTGGAGCGCTTCCCCATCAGCTTCAAGACCTACTTCTCAGGGAAC
  TACTTCCGCCACATCGTGCTGGGGGTGAACTTCGCGGGCCGCTACGGTGCGCTGGGCATG
  AGTCGGCGCGAGGACCTGATGTACAAGCCGCCCGCCTTCCGCACGCTCAGCGAGCTCGTG
  CTGGACTTCGAGGCCGCCTACGGCCGCTGCTGGCACGTGCTCAAGAAGGTGAAGCTGGGC
  CAGAGCGTGTCACACGACCCGCACAGCGTGGAGCAGATCGAGTGGAAGCACTCGGTGCTG
  GACGTGGAGCGCCTGGGCCGCGATGACTTCCGCAAGGAGCTGGAGCGCCACGCCCGCGAC
  ATGCGGCTCAAGATTGGCAAAGGGACGGGCCCTCCCTCTCCCACCAAGGACCGGAAGAAG
  GATGTTTCTTCCCCGCAGCGGGCCCAGTCCAGCCCCCACCGCAGGAACAGCCGCAGTGAA
  AGACGGCCCTCGGGTGACAAGAAGACTTCCGAGCCCAAAGCCATGCCAGACCTTAACGGG
  TACCAGATCCGGGTCTGA
  
  Clone variation with respect to NM_014909.4
  384 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014909 unedited
  GGTGGTGTNGGGGNNANNNNNNNNNTTTCTTTANNGTTTTTACACCCGCCCGTTGCCGCA
  AAGGGCGGTAGGCGTGTACGGTGGGNAGTCTATATAAGCAGAGCTCATTTAGGTGACACT
  ATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGCTCTTGT
  GAGCCAGTTGTTTTCCCGCCTCCACCACCCCCCTCGAAGATTTAGGGATGCCAGGGGGGA
  AGAAGGTGGCTGGGGGTGGCAGCAGCGGTGCCACTCCAACGTCCGCTGCGGCCACCGCCC
  CCTCTGGGGTCAGGCGTTTGGAGACCAGCGAAGGAACCTCAGCCCAGAGAGATGAGGAGC
  CAGAAGAGGAAGGGGAAGAGGACCTGCGAGACGGAGGCGTCCCCTTCTTTGTCAACCGGG
  GTGGGCTACCTGTGGATGAGGCCACCTGGGAAAGGATGTGGAAACACGTGGCCAAGATCC
  ACCCCGATGGAGAGAAGGTGGCGCAACGGATCCGTGGGGCCACAGACCTGCCCAAGATCC
  CCATACCGAGTGTGCCTACGTTCCAGCCGTCTACACCTGTCCCTGAGCGCCTGGAAGCTG
  TGCAGCGCTATATCAGAGAGCTGCAGTACAATCACACAGGGACACAGTTCTTTGAAATTA
  AGAAGAGCAGACCTCTGACAGGGCTGATGGACCTGGCCAAGGAAATGACCAAAGAGGCCC
  TGCCAATCAAATGCCTGGAAGCCGTGATCCTGGGGAATTTACCTCACCAACAGCATGCCC
  ACCCTGGAGCGCTTCCCCATCAGCTTCAAGACCTACTTCTCAGGGAACTACTTCCGCCAC
  ATCGGCCTGGGGGGGTGACCTTCGCGGGCCGCTACGGTGCGCTGGGCATGTAGTCGCGCG
  AGGACCTGATGTACAAGCCGNCCGCCTTTCGCACGCTCAGCGAGCTCGTGCTGGACTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014909
• Insert Size: 5500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014909.2, NP_055724.1
• RefSeq Size: 5747 bp
• RefSeq ORF: 1098 bp
• Locus ID: 22846
• UniProt ID: Q7L8A9
• Cytogenetics: 14q24.3
• Protein Families: Secreted Protein
• Gene Summary: Tyrosine carboxypeptidase that removes the C-terminal tyrosine residue of alpha-tubulin, thereby regulating microtubule dynamics and function (PubMed:29146869). Acts as an angiogenesis inhibitor: inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis (PubMed:15467828, PubMed:16488400, PubMed:16707096, PubMed:19204325). This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts (PubMed:15467828, PubMed:16488400, PubMed:16707096).[UniProtKB/Swiss-Prot Function]