YPEL5 (NM_016061) Human Untagged Clone

CAT#: SC114524

YPEL5 (untagged)-Human yippee-like 5 (Drosophila) (YPEL5), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: YPEL5
• Synonyms: CGI-127
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_016061 edited
  ATGGGCAGAATTTTCCTTGATCATATCGGTGGTACCCGTCTGTTTTCTTGTGCAAACTGT
  GATACGATCCTGACCAACCGCTCAGAACTCATCTCCACTCGTTTCACAGGCGCCACTGGC
  AGAGCATTTCTTTTTAACAAGGTAGTTAACCTGCAGTACAGCGAAGTTCAAGATCGGGTC
  ATGCTCACTGGCCGCCACATGGTTCGAGATGTGAGCTGCAAAAACTGCAATAGCAAACTG
  GGATGGATCTATGAGTTTGCCACTGAAGACAGCCAGCGATATAAGGAAGGCCGCGTGATC
  CTGGAACGTGCTCTAGTTCGAGAGAGTGAGGGCTTTGAGGAGCATGTACCATCTGATAAC
  TCTTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016061 unedited
  GTTCGCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTGGCCGAGG
  GGCTTCATACCAGCTGAAGAGCGACAAGCCGCTGGCAGCCGCGGATCTCACCGCCGCTCA
  GGGTTTTTAGAACTTCAGCCATAAAAATGGGCAGAATTTTCCTTGATCATATCGGTGGTA
  CCCGTCTGTTTTCTTGTGCAAACTGTGATACGATCCTGACCAACCGCTCAGAACTCATCT
  CCACTCGTTTCACAGGCGCCACTGGCAGAGCATTTCTTTTTAACAAGGTAGTTAACCTGC
  AGTACAGCGAAGTTCAAGATCGGGTCATGCTCACTGGCCGCCACATGGTTCGAGATGTGA
  GCTGCAAAAACTGCAATAGCAAACTGGGATGGATCTATGAGTTTGCCACTGAAGACAGCC
  AGCGATATAAGGAAGGCCGCGTGATCCTGGAACGTGCTCTAGTTCGAGAGAGTGAGGGCT
  TTGAGGAGCATGTACCATCTGATAACTCTTGAAGATACAGAGAGAAATCCATCTTTTCCC
  AGGTCTCCTTCACTGAAAACAAAAATCTACTTACATACACTGTCACCTTAGCATCAGAGT
  CGGATTAATGAACTGCGGAACAAGAGGTTGTGAGAATCTAAGATGGAACCTTTCTTTCTT
  TCTTTCTTTNTTTTTAAATTTTGTATTTTCCATCCAACAGCAGTGTGTAAAGAGAATATT
  ATGCAGATGCCGTTAATTTTTTACCCTATGTTTACATCTTGAGGCAGCAGAGTCTGTCTG
  CAGCTATGTGGTGAGCTATGTAAGGGAAAAAATCTGGGCTGTTAAAGTGAAAAGTGTGTT
  CTATGTTCATTGTGAAAGGAAAATGTTAGAGTATGGNTTTTAAACCTTGGCTCCATTTAA
  ACTTTTTTTTTAAACCCAGTTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016061
• Insert Size: 2680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016061.1, NP_057145.1
• RefSeq Size: 2183 bp
• RefSeq ORF: 366 bp
• Locus ID: 51646
• UniProt ID: P62699
• Cytogenetics: 2p23.1
• Domains: Yippee
• Gene Summary: Component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (PubMed:29911972). Required for normal cell proliferation (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) lacks two alternate exons, compared to variant 1. Variants 1, 2, 3, and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.