TBC1D7 (NM_016495) Human Untagged Clone

CAT#: SC114250

TBC1D7 (untagged)-Human TBC1 domain family, member 7 (TBC1D7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBC1D7
• Synonyms: MGCPH; PIG51; TBC7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114250 sequence for NM_016495 edited (data generated by NextGen Sequencing)
  ATGACTGAGGACTCTCAGAGAAACTTTCGTTCAGTATATTATGAGAAAGTGGGGTTTCGT
  GGAGTTGAAGAAAAGAAATCATTAGAAATTCTCCTAAAAGATGACCGTCTGGATACTGAG
  AAACTTTGTACTTTTAGTCAGAGGTTCCCTCTCCCGTCCATGTACCGTGCATTGGTATGG
  AAGGTGCTTCTAGGAATCTTGCCTCCACACCACGAGTCCCATGCCAAGGTGATGATGTAT
  CGTAAGGAGCAGTACTTGGATGTCCTTCATGCCCTGAAAGTCGTTCGCTTTGTTAGTGAT
  GCCACACCTCAGGCTGAAGTCTATCTCCGCATGTATCAGCTGGAGTCTGGGAAGTTACCT
  CGAAGTCCCTCTTTTCCACTGGAGCCAGATGATGAAGTGTTTCTTGCCATAGCTAAAGCC
  ATGGAGGAAATGGTGGAAGATAGTGTCGACTGTTACTGGATCACCCGACGCTTTGTGAAC
  CAATTAAATACCAAGTACCGGGATTCCTTGCCCCAGTTGCCAAAAGCGTTTGAACAATAC
  TTGAATCTGGAAGATGGCAGACTGCTGACTCATCTGAGGATGTGTTCCGCGGCGCCCAAA
  CTTCCTTATGATCTCTGGTTCAAGAGGTGCTTTGCGGGATGTTTGCCTGAATCCAGTTTA
  CAGAGGGTTTGGGATAAAGTTGTGAGTGGATCCTGTAAGATCCTAGTTTTTGTAGCTGTC
  GAAATTTTATTAACCTTTAAAATAAAAGTTATGGCACTGAACAGTGCAGAGAAGATAACA
  AAGTTTCTGGAAAATATTCCCCAGGACAGCTCAGACGCGATCGTGAGCAAGGCCATTGAC
  TTGTGGCACAAACACTGTGGGACCCCGGTCCATTCAAGCTGA
  
  Clone variation with respect to NM_016495.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016495 unedited
  NGGTCAGCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCCGGCCG
  TCTGGGCGCGCGCGGGTGTCCCAGGTACCCCGCCGCCTCCCTCCTTCCCGCAGGACTCGT
  GCCTCCTGGCCCTGGGCCCCAACGGTGCCCGCCTGCCCGGCGGTCGGTCAGCGCACAGGG
  CACGGCTTCCTCTGCTTCTCCCGGACCACTTAGTCTCAACCCGGGTGTGTTTGCACTACT
  AGAGAATGAAATATGACTGAGGACTCTCAGAGAAACTTTCGTTCAGTATATTATGAGAAA
  GTGGGGTTTCGTGGAGTTGAAGAAAAGAAATCATTAGAAATTCTCCTAAAAGATGACCGT
  CTGGATACTGAGAAACTTTGTACTTTTAGTCAGAGGTTCCCTCTCCCGTCCATGTACCGT
  GCATTGGTATGGAAGGTGCTTCTAGGAATCTTGCCTCCACACCACGAGTCCCATGCCAAG
  GTGATGATGTATCGTAAGGAGCAGTACTTGGATGTCCTTCATGCCCTGAAAGTCGTTCGC
  TTTGTTAGTGATGCCACACCTCAGGCTGAAGTCTATCTCCGCATGTATCAGCTGGAGTCT
  GGGAAGTTACCTCGAAGTCCCTCTTTTCCACTGGAGCCAGATGATGAAGTGTTTCTTGCC
  ATAGCTAAAGCCATGGAGGAAATGGTGGAAGATAGTGTCGACTGTTACTGGATCACCCGA
  CGCTNTGTGAACCAATTAAATACCAAGTACCGGGATTCCTTGCCCCAGTTGCCAAAAGCG
  TTTGAACAATACTTGAATCTGGAAGATGGCAGACTGCTGACTCATCTGAGGATGTGTTCC
  GCGGCGCCCAACTCCTTATGATCTCTGGNTCAAGAGTGCTTTGCGGGNAGTTNGCCTGAA
  TCCAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016495 unedited
  CGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTGCTTGCAGAATATTTATTTTTAGAT
  CTCTCAGCAGTACAGTAAGAGTCTTTCGTTTCAGATCAGCTGGCCTCCAGCCAAAGCAGC
  GAAGTCAGAGGAACTTCAGCCCCTAAGCCTTCCTGCTCCCTAAGAGTACCAGCGCTCCTG
  CCTGGCTCCGGCATAAATGGTTGGCAATAAGTCATTTTTTATTAAAATTTTAACTCTAGG
  CAGCAATTTTGCTTTAAAAGGTTTTTCTTTGGAGTGCATTTTTATTTTAAAAAATTAAAT
  ACTCTTGGATATGTTAGTATTCAGAAGCAGCAGATTTCAACAGCTCACACTTTCTCTGAA
  CTTTTTTTCAACAGATATTTCACACACTAAGGAGGGCCTGAATTCTCAGAATTGTACCTC
  TATAAGTTCATAGCTGCCCTCGTCTTTTAGGGGGTGGGTGGAAAATCCAACTCACCACTC
  AGGCTCTTGCAGGTCTGAAGGCTAAACCGGTGTGAAACAAAGGGTCCCCTTTCACTCACT
  ACTCCCAAAGCCGGGAACTATCCAGTGAAGAGTGGCCATGCCATTTACGTGGCACAAGGT
  GGCCCAGGGGTGATGAGCTGTTCACCTTCACCGGCCATTTCTCTCACTGCTTTAGTGCGT
  GGCACAGTGCACCCTAACATCCCACATTTAACACCCCATCAAGAAAATGTGAGTGCAGCA
  AATATCCAACCAGTACTTTGCTCCTAGAGCANTCAAACACATTTGTTCATAATGTTTCAT
  TCAACTGGCCCAANAGAAAAGAGTTAGGATTGTTAAAGCCTGTTTACACGAGAAACCTAA
  AAGCATTGGCTGGATTAACCGTATGCTCACAAAGCCAACTGAGTGTACAAGGAAGACCCT
  ATCTAAAACAACTGAAGACACCTGNGCTANTAAGACGCAGCAGCTATTATCCTGTGTTAA
  CCCCCTCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016495
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016495.2, NP_057579.1
• RefSeq Size: 1147 bp
• RefSeq ORF: 882 bp
• Locus ID: 51256
• UniProt ID: Q9P0N9
• Cytogenetics: 6p24.1
• Gene Summary: This gene encodes a member of the TBC-domain containing protein family. The encoded protein functions as a subunit of the tuberous sclerosis TSC1-TSC2 complex which plays a role in the regulation of cellular growth and differentiation. Mutations in this gene have been associated with autosomal recessive megalencephaly. Alternative splicing results in multiple transcript variants. Naturally occurring readthrough transcription occurs between this locus and downstream LOC100130357. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) encodes the longest isoform (a). Variants 1, 2, 3, and 6 encode the same isoform (a).