VPS37C (NM_017966) Human Untagged Clone

CAT#: SC113816

VPS37C (untagged)-Human vacuolar protein sorting 37 homolog C (S. cerevisiae) (VPS37C)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VPS37C
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_017966, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGACGCTGAAGGATAAGACCCTGCAGGAGCTGGAGGAGTTGCAGAATGACTCGGAGGCGATTGACC
  AGCTGGCCCTGGAGTCCCCTGAGGTCCAGGACCTACAGCTGGAACGGGAGATGGCACTGGCCACCAACCG
  GAGCCTGGCAGAGCGGAACTTGGAGTTCCAGGGTCCCCTGGAGATCAGCCGCTCAAACCTCTCGGATAGA
  TACCAGGAGCTCCGGAAGCTCGTGGAGCGGTGCCAGGAGCAGAAGGCAAAGCTGGAGAAATTTTCTTCAG
  CACTGCAGCCAGGGACCTTGTTAGACCTTCTGCAGGTGGAAGGCATGAAGATCGAAGAAGAGTCCGAGGC
  CATGGCTGAGAAGTTCCTGGAGGGCGAGGTGCCCCTGGAAACGTTCCTGGAGAATTTTTCCTCCATGAGG
  ATGCTGTCCCACCTGCGCCGGGTTCGCGTGGAAAAGCTCCAGGAAGTGGTGAGGAAGCCCAGGGCTTCCC
  AGGAGCTGGCCGGCGATGCCCCTCCACCCCGTCCACCACCCCCGGTGCGCCCAGTCCCCCAGGGAACACC
  CCCTGTGGTTGAAGAGCAGCCGCAGCCACCATTAGCCATGCCTCCCTACCCTTTGCCCTACAGCCCATCC
  CCCAGCCTGCCTGTGGGCCCCACTGCCCATGGAGCCCTGCCACCGGCCCCTTTCCCAGTAGTGTCCCAGC
  CCTCCTTCTACAGCGGGCCTCTGGGCCCCACTTACCCGGCAGCCCAGCTTGGACCCAGGGGTGCTGCGGG
  TTACTCCTGGTCCCCACAGAGGAGCATGCCACCCCGGCCGGGCTATCCTGGGACCCCAATGGGTGCCTCT
  GGGCCTGGGTACCCCTTGCGGGGAGGCAGGGCCCCCAGTCCTGGTTATCCTCAACAGTCCCCATACCCCG
  CAACAGGAGGAAAACCTCCCTACCCAATACAGCCTCAGCTCCCCAGCTTTCCAGGCCAGCCCCAGCCCTC
  AGTGCCCCTGCAGCCCCCTTATCCCCCCGGGCCCGCCCCTCCCTATGGGTTCCCACCACCGCCGGGGCCT
  GCCTGGCCTGGGTATTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017966 unedited
  TCTATAGGGCGGCCGCGAATTCGGCACGAGGGTCAGACAAGCTTGTCCTATGTCCTATTT
  GAGTGGCAGCAGCGCCAGCCCAGCAAGAAGGCTGGGGGTTGTCAAGGTTGTCCCCAGACC
  TTGCTTGCAGTGGTTGGAGAACCCAGGGGGCTGCCTTGGGCCCTCTGGCCAGAGGGAAGC
  GGGCAGCTCTAGCCCTGGAGATTGTGGTCACATTGGGGCTTGTTTAGGATTGGAGGGCCA
  GGTCACCTCCCCAGCCACCCTCCCTTCTCTCCTCTGGGGTCCCCACTTTAGGGCGACTTT
  GCCCGAGGCCCACGCATCCATCCACTCCTTTAGTGCCTTGAATCTCATTCACAAGCAGCC
  CCCTCCCTTCCCCTCCCCTTCTCACTCTGTTGATGTAATCCTCCCACCCCCAGTGTCCAT
  CCTAAGACAGGCATCAAAAGAGGCCCTAACTTTACTTCCCAAATGGTGCTTTNTAAAAAA
  CACCATCACTACATTAGGGGCAATTTTTTCACACCTCCCTGTCTTCAGAATGTANAGGGT
  GGGGATTATGTCTCTGTTTATATGCAGCCCCCTGCACTGTGGGGTTTGGGGCATGGTTCA
  GTATAAGAATGAATACCATAGACCAAGGGGGTGTGATGAGTGGTAACTTGGNTGNTCCAG
  AGGGTAACCANGTCTCAGGNAAGAGCCTGGAGCTGCTATTGCTTAGAAAGTGACGTGCCT
  TGAATGTCACTACCTGAGACGCNCATCTGTGGGCCCGGGCTGCTGGGGCCAAGAGGACCT
  GACCTTNTGGTGCTGCCCTCCCCGNCTTAGTGCATCCGGTGACGCTAGTCACGCTTGCGT
  TCTTACCAAGGGCTGCTCTCCTAGTTTTNATAGAGAATCCTATGGACCTACTGGTGATGC
  TCTGACTGAGACGACGCTGNATGTTGGNATAATGCCTATAACTTGAGAGCAAAAAAAAAA
  ACTGCTTAATGCGCGGGCTACTGTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017966 unedited
  NCCTACTTGNACGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTGACTCATC
  AAAGTTTATTATGGCAATTAATTACACAAACATACAGGCGGTCGGTCATCCAGCTCAGAA
  GCACTCAAACCAGTAGAGTCCATTAGTGATTTCTCTTTATAAATAACTTAGGAGAAGCAA
  GCACATTTGGTAAAGAACCGGCAGCGCTGACCTAGCGTCACGCGGATGGCACATAGGCCG
  GGGAGGGGCAGGCACCAGAAGGTCAAGGTCCCTCCTTGGCCCCAGCAGCCCCGGGCCCAC
  CAGATGCTGCGTCCTCAGGTAGTGGACATTTCAAGGCACGTACAACTTCCTAAGCAATAG
  CAGCTCCAGGCTCTTCCCTGAGACCTGGTTTACCTCTGGAACAACCAAGTTAACACTCAT
  CACACCCCCTTTGTCTATTGTATTCATTCTTATTACTGAACATGCCCCAAACCCCACAGT
  GCAGGGGGCTGCATATTAAACAGAGACATAATCCCCACCCTTTACATTCTGAAGACAGGG
  AGGTGTGAAAAAATTGCCCCTAATGTAGTGATGGTGTTTTTTAAAAAGCACCATTTGGGA
  AGTAAAGTTAGGGCCTCTTTTGATGCCTGTCTTAGGATGGACACTGGGGGTGGGGAGGGA
  TACATCAACAGAGTGAAAAGGGGAGGGNGAAGGGAGGGGCTGCCTGTGAATGANATTCAG
  GCACTAAAGGAGTGGATGGATGCGTGGGCCTCCGGNCAAGTCGCCCTTAAGTGGGGACCC
  CACAGGAANAAGGGGAGGTGGCTTGGGNAGGTGACCTGGCCCTTCCATATCTTAACAGCC
  CAATGTGACCACATTCTCCAGGGCTAGAGCTGGCCGCTTCCCTTTGGCCAAAGGGCCCAA
  GCAAGCCCCTGGGTTCTCCCACCACTGCAACCAGGTCTTGGGGAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017966
• Insert Size: 960 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017966.2, NP_060436.2
• RefSeq Size: 2711 bp
• RefSeq ORF: 486 bp
• Locus ID: 55048
• UniProt ID: A5D8V6
• Cytogenetics: 11q12.2
• Protein Pathways: Endocytosis
• Gene Summary: VPS37C is a subunit of ESCRT-I (endosomal sorting complex required for transport I), a complex in the class E vacuolar protein sorting (VPS) pathway required for sorting ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies (Eastman et al., 2005 [PubMed 15509564]).[supplied by OMIM, Mar 2008]