ZNF415 (NM_018355) Human Untagged Clone

CAT#: SC113567

ZNF415 (untagged)-Human zinc finger protein 415 (ZNF415), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZNF415
• Synonyms: Pact; ZfLp
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113567 sequence for NM_018355 edited (data generated by NextGen Sequencing)
  ATGGCTTTTACTCAGTTGACATTCAGGGACGTGGCCATCGAATTCTCTCAAGATGAGTGG
  AAATGCCTGAACTCTACACAGAGGACTTTATACAGGGATGTGATGTTGGAGAACTACAGG
  AACCTGGTCTCCCTGGATCTGTCTCGTAACTGTGTAATCAAGGAACTAGCACCACAACAG
  GAAGGTAACCCAGGAGAAGTATTCCACACAGTGACATTGGAACAACATGAAAAACATGAC
  ATTGAAGAGTTTTGCTTCAGGGAAATCAAGAAAAAAATACACGACTTTGACTGTCAGTGG
  AGAGATGATGAAAGAAATTGCAACAAAGTGACTACGGCCCCAAAAGAAAATCTTACTTGT
  AGGAGAGACCAACGCGATAGAAGAGGTATAGGAAACAAGTCTATTAAACATCAGCTTGGA
  TTAAGCTTTCTACCACATCCCCATGAACTGCAGCAGTTTCAAGCTGAAGGGAAAATTTAT
  GAATGTAACCATGTTGAGAAGTCTGTCAACCATGGTTCCTCAGTTTCACCACCCCAAATA
  ATTTCTTCTACCATCAAAACCCATGTTTCTAATAAATATGGGACTGATTTCATCTGTTCT
  TCATTACTCACACAAGAACAGAAATCATGCATTAGGGAAAAACCTTACAGATATATTGAG
  TGCGACAAAGCCTTGAATCATGGCTCACACATGACTGTACGTCAGGTAAGTCATTCTGGA
  GAGAAAGGATATAAATGTGATCTGTGTGGCAAGGTCTTTAGTCAAAAATCAAACCTTGCG
  CGTCATTGGAGAGTTCATACTGGAGAGAAACCATACAAATGTAATGAATGTGACAGAAGT
  TTCAGTCGCAACTCATGCCTTGCACTACATCGGAGAGTTCACACTGGAGAGAAACCTTAC
  AAATGTTATGAGTGTGACAAGGTCTTCAGTCGAAATTCATGCCTTGCACTACATCAGAAA
  ACTCATATTGGAGAGAAACCTTACACATGTAAAGAGTGTGGCAAAGCCTTTAGTGTGAGG
  TCAACACTTACCAACCATCAGGTAATTCATAGTGGCAAGAAACCTTACAAATGCAATGAA
  TGTGGCAAGGTGTTCAGTCAGACTTCAAGCCTTGCAACTCATCAGAGAATTCACACTGGG
  GAGAAACCATACAAGTGTAATGAATGTGGTAAAGTCTTCAGTCAGACTTCAAGCCTTGCA
  AGGCATTGGAGAATTCATACTGGAGAGAAACCTTACAAATGCAATGAATGTGGTAAGGTT
  TTCAGTTACAATTCACACCTTGCGAGTCATCGGAGAGTTCATACTGGAGAGAAACCTTAC
  AAGTGTAATGAGTGTGGGAAAGCCTTTAGTGTGCATTCGAACTTAACTACCCATCAGGTC
  ATCCATACTGGAGAGAAGCCTTACAAATGTAATCAATGTGGCAAAGGCTTCAGTGTGCAT
  TCAAGCCTAACTACCCATCAGGTCATCCATACTGGAGAAAAACCTTACAAATGTAATGAG
  TGTGGCAAATCCTTTAGTGTGCGCCCAAACCTCACTAGACATCAGATAATCCATACTGGA
  AAGAAACCTTACAAATGTAGTGATTGTGGGAAGTCCTTTAGTGTGCGCCCAAACCTCTTC
  AGACATCAAATTATCCATACTAAGGAGAAACCTTATAAAAGAAATTAA
  
  Clone variation with respect to NM_018355.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018355 unedited
  NTGTTCAAATTTTNGTATACGACTCATATAGGCGGCCCGCGAATTCGCACGAGGAACGGA
  TCGCGTTGGGTGAAGGTGACGGCGTCGAGCGACCTCTCTGACCTCATCTCCTGCCTGTGT
  CCTCCTCGCTCCCTCTGCTCCAGCCAAGCATGCGTCCTTCCTGTTCCTGGGCCGAGCTTG
  CTCCTGCCTCAAGGCCTTCACGTGGGCTGTGCCTCTGCTGGAACTCGCTGGCCCCTCAGC
  TGCAGCATTGACTTCCAAAGACTCCTGGCACATGAGGAAGAAACCCAGAAGAGGAGAGCA
  AAGGAGTCAGGAATGGCTTTTACTCAGTTGACATTCAGGGACGTGGCCATCGAATTCTCT
  CAAGATGAGTGGAAATGCCTGAACTCTACACAGAGGACTTTATACAGGGATGTGATGTTG
  GAGAACTACAGGAACCTGGTCTCCCTGGATCTGTCTCGTAACTGTGTAATCAAGGAACTA
  GCACCACAACAGGAAGGTAACCCAGGAGAAGTATTCCACACAGTGACATTGGAACAACAT
  GAAAAACATGACATTGAAGAGTTTTGCTTCAGGGAAATCAAGAAAAAAATACACGACTTT
  GACTGTCAGTGGAGAGATGATGAAAGAAATTGCAACAAAGTGACTACGGCCCCAAAAGAA
  AATCTCACTTGTAGGAGAGACCAACGCGATAGAAGAGGTATAGGAAACAAGTCTATTAAA
  CATCAGCTTGGATTAAGCTNTCTACCACATNCCCATGAACTGCAGCAGTTTCAAGCTGAA
  GGGAAAAATTATGAATGTAACCCATGTGAGAAGTCTGTCACCCATGGNTCCCTCAGTTCA
  CCACCCCAAATAAATTCTTCTACATCAAAACCCCATGTTCTAATAATATGGGACTGATTN
  CATCTGTTCTTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018355 unedited
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTAAAAACACCCTGCACCCTTAAATATATATAAGGTGGGGGGAGTTTAA
  ACATATTGGGGCTTGCTTTTGTCCTACAAGGGGAAAAATAAAAAGTTTATCACCCCACCT
  AAACTGAATTTTCCCTCACAAAAACCCTGGGATGGGTAACAGTTTGGACCCTGGGTAAAA
  ACCTCCAACATGCCCAAAATATACAAAAATGTTCCCCCATTTTGATGTCTAGGGAGTGGG
  GAAACCTAAAGAAACCCTTTGCCACACAGTAAGGGGTATATAATTTCTTTTTACCAGGGC
  CTCTGATGCCAAAAAAGGCTTAACCTTGGAAAAAAGGGTTGGCTGACCTCGAAAGGATCC
  TCTAAAAAATGAAATCCTCTGAGCCTGGGAAGGAGGAAATTGGAACTGAAACCCTTGCCA
  CATTTATTATCCTGGAAGGGTTCCCCCCTGATAAAAATCCTTGGAGGACCCACAGGATTA
  AAACTTTGACGGAAAACCTTGCCATATAAATTTCTTTTAAAGGGGCTCCCCTTAAATGGG
  ATAATTTGATGCCCGAAAAGGGCGGGGCCCCCACAAAAGATCTCCCCAAATACCTCTTTG
  GAAAGGCTCCTTCCCATTTGGACTTCCCGAGCGCCAGGGAGGGTGGGCCCCACCCTAAGG
  ATTCGCCCCCCTCATTCATTTGAAAGGTTTTCTCCCCTCGGGCGACCCGGGGGTAAAAGG
  CTTGAACCCCCCGAAACCCTTCCCCCATGAAACATTTGAAGGGTCTCCTCCAGCGGGGAG
  ACCTGGGGGTATTAATTCAAAGCCCACAAGGCTTCCCCCCTTTCCCTTGAGGGTCTCTCC
  GATAACCCCCCACCCCCGGGGGAATGACCAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018355
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018355.2, NP_060825.2
• RefSeq Size: 2287 bp
• RefSeq ORF: 1668 bp
• Locus ID: 55786
• UniProt ID: Q09FC8
• Cytogenetics: 19q13.42
• Domains: KRAB, zf-C2H2
• Protein Families: Transcription Factors
• Gene Summary: Involved in transcriptional regulation. Transcriptional activity differed among the various isoforms. All isoforms except isoform 3 seem to suppresses the transcriptional activities of AP-1 and p53/TP53.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR and an alternate exon containing a distinct start codon, compared to variant 1. The resulting isoform (2) is the same length as but has a distinct N-terminus, compared to isoform 1. Variants 2, 3 and 21 encode the same protein (isoform 2).