TCIM (NM_020130) Human Untagged Clone

CAT#: SC113184

C8orf4 (untagged)-Human chromosome 8 open reading frame 4 (C8orf4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCIM
• Synonyms: C8orf4; TC-1; TC1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113184 sequence for NM_020130 edited (data generated by NextGen Sequencing)
  ATGAAAGCAAAGCGAAGCCACCAAGCCATCATCATGTCCACGTCGCTACGAGTCAGCCCA
  TCCATCCATGGCTACCACTTCGACACAGCCTCTCGTAAGAAAGCCGTGGGCAACATCTTT
  GAAAACACAGACCAAGAATCACTAGAAAGGCTCTTCAGAAACTCTGGAGACAAGAAAGCA
  GAGGAGAGAGCCAAGATCATTTTTGCCATAGATCAAGATGTGGAGGAGAAAACGCGTGCC
  CTGATGGCCTTGAAGAAGAGGACAAAAGACAAGCTTTTCCAGTTTCTGAAACTGCGGAAA
  TATTCCATCAAAGTTCACTGA
  
  Clone variation with respect to NM_020130.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020130 unedited
  ATATACACCCGCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGNGAGTCTATATA
  AGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC
  GCGAATTCGGCACGAGGGCTGAATTCCGGAAGATCCCCACATCGATGAAAGCAAAGCGAA
  GCCACCAAGCCATCATCATGTCCACGTCGCTACGAGTCAGCCCATCCATCCATGGCTACC
  ACTTCGACACAGCCTCTCGTAAGAAAGCCGTGGGCAACATCTTTGAAAACACAGACCAAG
  AATCACTAGAAAGGCTCTTCAGAAACTCTGGAGACAAGAAAGCAGAGGAGAGAGCCAAGA
  TCATTTTTGCCATAGATCAAGATGTGGAGGAGAAAACGCGTGCCCTGATGGCCTTGAAGA
  AGAGGACAAAAGACAAGCTTTTCCAGTTTCTGAAACTGCGGAAATATTCCATCAAAGTTC
  ACTGAAGAGAAGAGGATGGATAAGGACGTTATCCAAGAATGGACATTCAAAGACCAAGTG
  AGTTTGTGAGATTCTAACAGATGCAGCATTTTGCTGCTACCTTACAAGCTTCTCTTCTGT
  CAGGACTCCAGAGGCTGGAAAGGGACCGGGNACTGAAAGGGACCANNGACTGACAGACTG
  GGTACAAAGACTNNCCAACATTTCATGCCNTGTGCTGNNTACAGAGNAGAACAAAAGTGC
  TTCAGCAAGGATTTTGAAAACTCTCCCGTCCCTGCAGNAAAAGATTGATGCTGATAGAAG
  AGCTGNNACGATGTATGAGAACTAAAGAAACCGATGGCTGTAGATGACTTTATNCAGGTC
  CACTTGTCAGGCCTTAGGACTTAAATCGAAGTGAACTTTTTTTTTNTTTTTTAACCCAAT
  ATATAGGAAAGGNNAGAGGAAAGGGAGACACG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020130 unedited
  NNNNAACGGTCCTAGCTATGNNACCGCGGCCGCATNCTAGNGATCGATTTTTTTTTTTTT
  TTTTTTAAGTTTACAATAATTTATTATTGTTGCATGACATTTGCCAGTAAAATAAATTAT
  AGAAACTATAGAGTCTTTATAAACTATTTTGTATATCATATTCACTTCCTAATGCTTACT
  GCAGTAACTGTATGAAATTTAATTAGATTACGTTTTAGCATTAGTCAGAAGATTTAAAAA
  ATATGTAAAATGTTTTCACAGTACTTTGGATTTATAAAAGACCCCATTATTTTAACTTTT
  GTGCAACCTGTTTGAAATGTATAAAAAACCTTTTACAAACCAAAAGGTGGCGTAAGGTTT
  TACTGAGTTGCTGAAGACATCTTACTTTCTTGAATTTCTACTTAACATCCATGTGGGGCA
  CTTTTTCAGGCATTGTAATAAGTGCCAATAAATAATCAATTATTGATTTCTAAAAATCTT
  TTCCAATAGACAATACTCAAGCTTGGAAATATTTTGAACACCTCGATATAAAAATTCAGT
  AAACAATTTATGCCTGGGATTTTCTCTCCCTGGCCTCCCTCTCCCCCCTCCCTTTCCCTA
  TTTATTTTGCTAAAAAAAAAAAAAAAAGGTCAACCTCCCATTTAATTCCTCGGGCCCGTC
  ACAAGGCCCCTGGATAAAAGTCTTTTAACAGCCACTGGTTTTCCTTACAGTTAACATAAA
  CCTTGCCCCGCCTCTTTTTAACCCCTTAACACCCTTCCCCCCCGGCGCCGTAAAAATGTT
  CTTAATTTCCGCTGGAACGCATTTTGCTTATCCCTTTCTACCCGACGGGGCCTGACAATT
  CTTGTGAGATCGTTCGAACCCCCCGTTTGTACCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020130
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020130.2, NP_064515.1
• RefSeq Size: 1488 bp
• RefSeq ORF: 321 bp
• Locus ID: 56892
• UniProt ID: Q9NR00
• Cytogenetics: 8p11.21
• Gene Summary: This gene encodes a small, monomeric, predominantly unstructured protein that functions as a positive regulator of the Wnt/beta-catenin signaling pathway. This protein interacts with a repressor of beta-catenin mediated transcription at nuclear speckles. It is thought to competitively block interactions of the repressor with beta-catenin, resulting in up-regulation of beta-catenin target genes. The encoded protein may also play a role in the NF-kappaB and ERK1/2 signaling pathways. Expression of this gene may play a role in the proliferation of several types of cancer including thyroid cancer, breast cancer and hematological malignancies. [provided by RefSeq, Nov 2011]