TLR3 (NM_003265) Human Untagged Clone

CAT#: SC112723

TLR3 (untagged)-Human toll-like receptor 3 (TLR3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TLR3
• Synonyms: CD283; IIAE2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003265 edited
  GAATTCGGCACGAGGGAAATGTCTGGATTTGGACTAAAGAAAAAAGGAAAGGCTAGCAGT
  CATCCAAAAGAATCATGAGACAGACTTTGCCTTGTATCTACTTTTGGGGGGGCCTTTTGC
  CCTTTGGGATGCTGTGTGCATCCTCCACCACCAAGTGCACTGTTAGCCATGAAGTTGCTG
  ACTGCAGCCACCTGAAGTTGACTCAGGTACCCGATGATCTACCCACAAACATAACAGTGT
  TGAACCTTACCCATAATCAACTCAGAAGATTACCAGCCGCCAACTTCACAAGGTATAGCC
  AGCTAACTAGCTTGGATGTAGGATTTAACACCATCTCAAAACTGGAGCCAGAATTGTGCC
  AGAAACTTCCCATGTTAAAAGTTTTGAACCTCCAGCACAATGAGCTATCTCAACTTTCTG
  ATAAAACCTTTGCCTTCTGCACGAATTTGACTGAACTCCATCTCATGTCCAACTCAATCC
  AGAAAATTAAAAATAATCCCTTTGTCAAGCAGAAGAATTTAATCACATTAGATCTGTCTC
  ATAATGGCTTGTCATCTACAAAATTAGGAACTCAGGTTCAGCTGGAAAATCTCCAAGAGC
  TTCTATTATCAAACAATAAAATTCAAGCGCTAAAAAGTGAAGAACTGGATATCTTTGCCA
  ATTCATCTTTAAAAAAATTAGAGTTGTCATCGAATCAAATTAAAGAGTTTTCTCCAGGGT
  GTTTTCACGCAATTGGAAGATTATTTGGCCTCTTTCTGAACAATGTCCAGCTGGGTCCCA
  GCCTTACAGAGAAGCTATGTTTGGAATTAGCAAACACAAGCATTCGGAATCTGTCTCTGA
  GTAACAGCCAGCTGTCCACCACCAGCAATACAACTTTCTTGGGACTAAAGTGGACAAATC
  TCACTATGCTCGATCTTTCCTACAACAACTTAAATGTGGTTGGTAACGATTCCTTTGCTT
  GGCTTCCACAACTAGAATATTTCTTCCTAGAGTATAATAATATACAGCATTTGTTTTCTC
  ACTCTTTGCACGGGCTTTTCAATGTGAGGTACCTGAATTTGAAACGGTCTTTTACTAAAC
  AAAGTATTTCCCTTGCCTCACTCCCCAAGATTGATGATTTTTCTTTTCAGTGGCTAAAAT
  GTTTGGAGCACCTTAACATGGAAGATAATGATATTCCAGGCATAAAAAGCAATATGTTCA
  CAGGATTGATAAACCTGAAATACTTAAGTCTATCCAACTCCTTTACAAGTTTGCGAACTT
  TGACAAATGAAACATTTGTATCACTTGCTCATTCTCCCTTACACATACTCAACCTAACCA
  AGAATAAAATCTCAAAAATAGAGAGTGATGCTTTCTCTTGGTTGGGCCACCTAGAAGTAC
  TTGACCTGGGCCTTAATGAAATTGGGCAAGAACTCACAGGCCAGGAATGGAGAGGTCTAG
  AAAATATTTTTGAAATCTATCTTTCCTACAACAAGTACCTGCAGCTGACTAGGAACTCCT
  TTGCCTTGGTCCCAAGCCTTCAACGACTGATGCTCCGAAGGGTGGCCCTTAAAAATGTGG
  ATAGCTCTCCTTCACCATTCCAGCCTCTTCGTAACTTGACCATTCTGGATCTAAGCAACA
  ACAACATAGCCAACATAAATGATGACATGTTGGAGGGTCTTGAGAAACTAGAAATTCTCG
  ATTTGCAGCATAACAACTTAGCACGGCTCTGGAAACACGCAAACCCTGGTGGTCCCATTT
  ATTTCCTAAAGGGTCTGTCTCACCTCCACATCCTTAACTTGGAGTCCAACGGCTTTGACG
  AGATCCCAGTTGAGGTCTTCAAGGATTTATTTGAACTAAAGATCATCGATTTAGGATTGA
  ATAATTTAAACACACTTCCAGCATCTGTCTTTAATAATCAGGTGTCTCTAAAGTCATTGA
  ACCTTCAGAAGAATCTCATAACATCCGTTGAGAAGAAGGTTTTCGGGCCAGCTTTCAGGA
  ACCTGACTGAGTTAGATATGCGCTTTAATCCCTTTGATTGCACGTGTGAAAGTATTGCCT
  GGTTTGTTAATTGGATTAACGAGACCCATACCAACATCCCTGAGCTGTCAAGCCACTACC
  TTTGCAACACTCCACCTCACTATCATGGGTTCCCAGTGAGACTTTTTGATACATCATCTT
  GCAAAGACAGTGCCCCCTTTGAACTCTTTTTCATGATCAATACCAGTATCCTGTTGATTT
  TTATCTTTATTGTACTTCTCATCCACTTTGAGGGCTGGAGGATATCTTTTTATTGGAATG
  TTTCAGTACATCGAGTTCTTGGTTTCAAAGAAATAGACAGACAGACAGAACAGTTTGAAT
  ATGCAGCATATATAATTCATGCCTATAAAGATAAGGATTGGGTCTGGGAACATTTCTCTT
  CAATGGAAAAGGAAGACCAATCTCTCAAATTTTGTCTGGAAGAAAGGGACTTTGAGGCGG
  GTGTTTTTGAACTAGAAGCAATTGTTAACAGCATCAAAAGAAGCAGAAAAATTATTTTTG
  TTATAACACACCATCTATTAAAAGACCCATTATGCAAAAGATTCAAGGTACATCATGCAG
  TTCAACAAGCTATTGAACAAAATCTGGATTCCATTATATTGGTTTTCCTTGAGGAGATTC
  CAGATTATAAACTGAACCATGCACTCTGTTTGCGAAGAGGAATGTTTAAATCTCACTGCA
  TCTTGAACTGGCCAGTTCAGAAAGAACGGATAGGTGCCTTTCGTCATAAATTGCAAGTAG
  CACTTGGATCCAAAAACTCTGTACATTAAATTTATTTAAATATTCAATTAGCAAAGGAGA
  AACTTTCTCAATTTAAAAAGTTCTATGGCAAATTTAAGTTTTCCATAAAGGTGTTATAAT
  TTGTTTATTCATATTTGTAAATGATTATATTCTATCACAATTACATCTCTTCTAGGAAAA
  TGTGTCTCCTTATTTCAGGCCTATTTTTGACAATTGACTTAATTTTACCCAAAATAAAAC
  ATATAAGCACGTAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003265 unedited
  TAGGGCGGCAACGCCAATTCGCACGAGGAAATGTCTGGATTTGGACTAAAGAAAAAAGGA
  AAGGCTAGCAGTCATCCAAAAGAATCATGAGACAGACTTTGCCTTGTATCTACTTTTGGG
  GGGGCCTTTTGCCCTTTGGGATGCTGTGTGCATCCTCCACCACCAAGTGCACTGTTAGCC
  ATGATATTTTCTGACTGCAGCCACCTGAAGTTGACTCAGGTACCCGATGATCTACCCACA
  AACATAACAGTGTTGAACCTTACCCATAATCAACTCAGAAGATTACCAGCCGCCAACTTC
  ACAAGGTATAGCCAGCTAACTAGCTTGGATGTAGGATTTAACACCATCTCAAAACTGGAG
  CCAGAATTGTGCCAGAAACTTCCCATGTTAAAAGTTTTGAACCTCCAGCACAATGAGCTA
  TCTCAACTTTCTGATAAAACCTTTGCCTTCTGCACGAATTTGACTGAACTCCATCTCATG
  TCCAACTCAATCCAGAAAATTAAAAATAATCCCTTTGTCAAGCAGAAGAATTTAATCACA
  TTAGATCTGTCTCATAATGGCTTGTCATCTACAAAATTAGGAACTCAGGTTCAGCTGGAA
  AATCTCCAAGAGCTTCTATTATCAAACAATAAAATTCAAGCGCTAAAAAGTGAAGAACTG
  GATATCTTTGCCAATTCATCTTTAANAAAATTAGAGTTGTCATCGAATCAAATTAAAGAG
  TTTTCTCCAGGGTGTTTTCACGCCATTGGAAGATTATTTGGCCTCTTTCTGAACATGTCC
  AGCTGGGTCCCAGCCTTACAGAGAAGCTATGTTTGGAATTAGCANNACACAGCATTCGGA
  ATCTGTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003265 unedited
  TAGAGTCGAGTTTTTTTTTTTTTTTTTTACGTGCTTATATGTTTTATTTTGGGTAAAATT
  AAGTCAATTGTCAAAAATAGGCCTGAAATAAGGAGACACATTTTCCTAGAAGAGATGTAA
  TTGTGATAGAATATAATCATTTACAAATATGAATAAACAAATTATAACACCTTTATGGAA
  AACTTAAATTTGCCATAGAACTTTTTAAATTGAGAAAGTTTCTCCTTTGCTAATTGAATA
  TTTAAATAAATTTAATGTACAGAGTTTTTGGATCCAAGTGCTACTTGCAATTTATGACGA
  AAGGCACCTATCCGTTCTTTCTGAACTGGCCAGTTCAAGATGCAGTGAGATTTAAACATT
  CCTCTTCGCAAACAGAGTGCATGGTTCAGTTTATAATCTGGAATCTCCTCAAGGAAAACC
  AATATAATGGAATCCAGATTTTGTTCAATAGCTTGTTGAACTGCATGATGTACCTTGAAT
  CTTTTGCATAATGGGTCTTTTAATAGATGGTGTGTTATAACAAAAATAATTTTTCTGCTT
  CTTTTGATGCTGTTAACAATTGCTTCTAGTTCAAAAACACCCGCCTCAAAGTCCCTTTCT
  TCCAGACAAAATTTGAGAGATTGGTCTTCCTTTTCCATTGAAGAGAATGTTCCAGACCCA
  ATCTTATCTTTATAGGCATGAATATATATGCTGATATTCAACTGGTCTGGCTGCTGGCTA
  ATTCTTGAAACCAAAACTCGATGACTGAACATTNCATAAAGATATCTCAGCCTCAAGTGA
  TGAGAGTCATAAGATAAATCACAGATCTGTTTGACATGAAAAAGTCAGGGGCACTGCTGC
  AGAGATGTTCAAAGCTCTGGAACATGANTGAGGGGATGTCAAGTATGCTGACCTAGAGTG
  NTTGGTCTGTATCATACACAGCAACTCACGTGCTAGATAAGCTTTATCNCGTCTGAGGGC
  GACTTTTAGGGGTAATCTTGAGTAGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003265
• Insert Size: 3190 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003265.2, NP_003256.1
• RefSeq Size: 3057 bp
• RefSeq ORF: 2715 bp
• Locus ID: 7098
• UniProt ID: O15455
• Cytogenetics: 4q35.1
• Domains: TIR, LRRCT, LRR, LRR_TYP, LRR_PS
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Toll-like receptor signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. [provided by RefSeq, Jul 2008]