RMND5A (NM_022780) Human Untagged Clone

CAT#: SC112425

RMND5A (untagged)-Human required for meiotic nuclear division 5 homolog A (S. cerevisiae) (RMND5A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RMND5A
• Synonyms: CTLH; GID2; GID2A; p44CTLH; RMD5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112425 sequence for NM_022780 edited (data generated by NextGen Sequencing)
  ATGGATCAGTGCGTGACGGTGGAGCGCGAGCTGGAGAAGGTGCTGCACAAGTTCTCAGGC
  TACGGGCAGCTGTGCGAGCGCGGCCTGGAGGAGCTCATCGACTACACCGGCGGCCTCAAG
  CACGAGATCCTGCAGAGCCACGGCCAAGATGCTGAATTATCAGGGACACTTTCACTTGTT
  TTGACACAGTGCTGTAAAAGAATAAAGGATACTGTTCAAAAATTGGCCTCCGACCACAAA
  GACATCCACAGCAGTGTTTCTCGGGTTGGAAAAGCCATTGATAAGAATTTTGATTCTGAC
  ATTAGCAGTGTGGGAATAGATGGCTGCTGGCAGGCAGACAGCCAAAGGCTTCTCAATGAG
  GTGATGGTGGAGCACTTCTTTCGACAAGGAATGCTGGATGTGGCTGAGGAGCTCTGTCAG
  GAATCTGGTCTTTCTGTAGACCCAAGTCAGAAGGAACCATTTGTGGAGTTAAATAGAATA
  TTAGAGGCATTAAAGGTCAGAGTTCTGAGACCTGCTCTGGAGTGGGCAGTGTCAAACCGG
  GAAATGCTTATAGCTCAAAACAGCTCCTTGGAATTTAAGCTACACAGACTGTATTTTATT
  AGCTTGTTAATGGGTGGAACCACAAATCAGCGAGAGGCATTACAATATGCTAAAAATTTT
  CAGCCATTTGCCCTAAATCATCAAAAAGACATTCAGGTTTTGATGGGAAGCCTTGTGTAC
  CTGAGACAAGGGATTGAGAACTCACCATATGTTCACCTACTTGATGCAAACCAGTGGGCT
  GATATCTGTGACATCTTTACACGGGATGCTTGTGCCCTCCTGGGGCTCTCCGTGGAGTCC
  CCTCTCAGTGTCAGTTTCTCAGCAGGTTGTGTGGCGCTGCCAGCTTTAATTAACATCAAA
  GCCGTGATTGAACAGAGGCAGTGTACTGGAGTTTGGAACCAGAAAGATGAATTACCTATT
  GAAGTGGACCTTGGTAAAAAGTGCTGGTATCACTCTATATTTGCCTGCCCCATTCTTCGT
  CAGCAAACAACAGATAACAATCCACCCATGAAATTGGTCTGTGGTCATATTATATCAAGA
  GATGCCCTGAATAAAATGTTTAATGGTAGCAAATTAAAATGTCCCTACTGTCCAATGGAA
  CAAAGTCCAGGAGATGCCAAACAGATATTTTTCTGA
  
  Clone variation with respect to NM_022780.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022780 unedited
  TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCGGTTCCCGTG
  AAAACAAAAACAATCGGCCGCGCCGTCGCAGGCACCCGAACGTCGCGAGCGGGGCCTGGG
  GACGCGGAGCCGAGTGCAGCGAGCGAACGGGAGCAGCGGCGACTCGCCGGGGGGCTAGGG
  CGCCATGGGGCAGGCGGGCTCCGGCTGCGCGGGGCTCCCCCGGCGCCGCGGCTAGTGCGC
  CCGCCGCCTCGGCCGCCTCAGCCTCCCGCGCCGCCCGCTTGGGGAACGAGGAGCAGGACG
  CGGCCTCGGTGGGGCCCGGGCCGAACGGCTGCGGACACCTGGGCGCCGAGGAGCCGAGCG
  CCGCCGTCTCCGGCATGGATCAGTGCGTGACGGTGGAGCGCGAGCTGGAGAAGGTGCTGC
  ACAAGTTCTCAGGCTACGGGCAGCTGTGCGAGCGCGGCCTGGAGGAGCTCATCGACTACA
  CCGGCGGCCTCAAGCACGAGATCCTGCAGAGCCACGGCCAAGATGCTGAATTATCAGGGA
  CACTTTCACTTGTTTTGACACAGTGCTGTAAAAGAATAAAGGATACTGTTCAAAAATTGG
  CCTCCGACCACAAAGACATCCACAGCAGTGTTTCTCGGGTTGGAAAAGCCATTGATAAGA
  ATTTTGATTCTGACATTAGCAGTGTGGGAATAGATGGCTGCTGGCANGCAGACAGCCAAA
  GGCTTCTCAATGAGGTGATGGTGGGAGCACTTCTTTCGACAAGGAATGCTGGATGTGGCT
  GAGGAGCTCTGCAGGAATCTGGTCTTTCTGTAGACCCAGTCAGAAGACCANTTGTGGGAG
  TAAAAGATATTAAGCCATAGGGGGGNNAAATTCTGAGACTGCTTTGGGAGGGGGCATGTC
  AACCGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022780 unedited
  NTTTAGCTCTGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTAACTTTAAAATTTCATTTTATTAAACATACTTTTAAAACAAGCACCTCTTG
  GCAATAAAAGCAAAAACAAAAAACAAACAAACAAAAACCCAACCAAACAATAGTACTCCT
  TCCACTCTATGCTAACGGAAGACTTCTCACACCAGCCAGTTAAACAATGAAATTCTTAAA
  CACGCAGCCTGCTGGGGCTGCATGCAAAGCTAAAATGCAGGTGTGCTGACTTCTTGGAGC
  TGGAGCAGAGGAAAACATCAAAAAGCATATCTGGAATCTATCACAGCTTTCTTTCTTAAG
  CAAATAAAAATGCAAATTAGTTTCATAACCACAATTCAATTTATCAAACTTTTTCTGAAG
  AATTTTCATTTAATTATGTATACATAACAGGAAATAAAACTTTTTCACAAACACTCTCAA
  GGCTTACGATTATCAAGAAAATGACAAAAGTAAAAGCAGGAGGAATATTAAACACATGGC
  GGGGAGGTCAATTTCCGTTTCTGCTCTTAAAAAGTAAAAAAATCACCATATAATTAAGTA
  CAAAAACCTGCATTGAATGACAACTGCTGGTGTATTTACTTGATCAGAAATGAAGACCAA
  GAATATAATAAAGCCCTATGATAATATTCACCGGGAGCACTCCCCTCAGTGTGTGTACAC
  TAACAGGNTGGGCCAAGTTCTGGAGCATTAGCTTATAGAAACACAGGAGTCCTTGGNTAG
  CTGCATTATATGGGACGTTTCTCTCCTGAATGCACCCACGATTCAGTTTCACTTACAAAT
  TGCAAACTAAAGTTATCTCTTCAGAAAAATATCTGTTGGCATCTCCTGAACTTTGTCCAT
  TGGAAGNAGGGACATTTAANTTGCTACCATTAACCATTTATCAAGGCATCTCTGAAATAT
  ATGACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022780
• Insert Size: 2400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_022780.2, NP_073617.1
• RefSeq Size: 3239 bp
• RefSeq ORF: 1176 bp
• Locus ID: 64795
• UniProt ID: Q9H871
• Cytogenetics: 2p11.2
• Domains: LisH, CTLH
• Gene Summary: Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. MAEA and RMND5A are both required for catalytic activity of the CTLH E3 ubiquitin-protein ligase complex (PubMed:29911972). Catalytic activity of the complex is required for normal cell proliferation (PubMed:29911972). The CTLH E3 ubiquitin-protein ligase complex is not required for the degradation of enzymes involved in gluconeogenesis, such as FBP1 (PubMed:29911972).[UniProtKB/Swiss-Prot Function]