FKRP (NM_024301) Human Untagged Clone

CAT#: SC112266

FKRP (untagged)-Human fukutin related protein (FKRP), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FKRP
• Synonyms: FKTR; LGMD2I; LGMDR9; MDC1C; MDDGA5; MDDGB5; MDDGC5
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024301, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGGCTCACCCGCTGCCAGGCTGCCCTGGCGGCCGCCATCACCCTCAACCTTCTGGTCCTCTTCTATG
  TCTCGTGGCTGCAGCACCAGCCTAGGAATTCCCGGGCCCGGGGGCCCCGTCGTGCCTCTGCTGCCGGCCC
  CCGTGTCACCGTCCTGGTGCGGGAGTTCGAGGCATTTGACAACGCGGTGCCCGAGCTGGTAGACTCCTTC
  CTGCAGCAAGACCCAGCCCAGCCCGTGGTGGTGGCAGCCGACACGCTCCCCTACCCGCCCCTGGCCCTGC
  CCCGCATCCCCAACGTGCGTCTGGCGCTGCTCCAGCCCGCCCTGGACCGGCCAGCCGCAGCCTCGCGCCC
  GGAGACCTACGTGGCCACCGAGTTTGTGGCCCTAGTACCTGATGGGGCGCGGGCTGAGGCACCTGGCCTG
  CTGGAGCGCATGGTGGAGGCGCTCCGCGCAGGAAGCGCACGTCTGGTGGCCGCCCCGGTTGCCACGGCCA
  ACCCTGCCAGGTGCCTGGCCCTGAACGTCAGCCTGCGAGAGTGGACCGCCCGCTATGGCGCAGCCCCCGC
  CGCGCCCCGCTGCGACGCCCTGGACGGAGATGCTGTGGTGCTCCTGCGCGCCCGCGACCTCTTCAACCTC
  TCGGCGCCCCTGGCCCGGCCGGTGGGCACCAGCCTCTTTCTGCAGACCGCCCTTCGCGGCTGGGCGGTGC
  AGCTGCTGGACTTGACCTTCGCCGCGGCGCGCCAGCCCCCGCTGGCCACGGCCCACGCGCGCTGGAAGGC
  TGAGCGCGAGGGACGCGCTCGGCGGGCGGCGCTGCTCCGCGCGCTGGGCATCCGCCTAGTGAGCTGGGAA
  GGCGGGCGGCTGGAGTGGTTCGGCTGCAACAAGGAGACCACGCGCTGCTTCGGAACCGTGGTGGGCGACA
  CGCCCGCCTACCTCTACGAGGAGCGCTGGACGCCCCCCTGCTGCCTGCGCGCGCTGCGCGAGACCGCCCG
  CTATGTGGTGGGCGTGCTGGAGGCTGCGGGCGTGCGCTACTGGCTCGAGGGCGGCTCACTGCTGGGGGCC
  GCCCGCCACGGGGACATCATCCCATGGGACTACGACGTGGACCTGGGCATCTACTTGGAGGACGTGGGCA
  ACTGCGAGCAGCTGCGGGGGGCAGAGGCCGGCTCGGTGGTGGATGAGCGCGGCTTCGTATGGGAGAAGGC
  GGTCGAGGGCGACTTTTTCCGCGTGCAGTACAGCGAAAGCAACCACTTGCACGTGGACCTGTGGCCCTTC
  TACCCCCGCAATGGCGTCATGACCAAGGACACGTGGCTGGACCACCGGCAGGATGTGGAGTTTCCCGAGC
  ACTTCCTGCAGCCGCTGGTGCCCCTGCCCTTTGCCGGCTTCGTGGCGCAGGCGCCTAACAACTACCGCCG
  CTTCCTGGAGCTCAAGTTCGGGCCCGGGGTCATCGAGAACCCCCAGTACCCCAACCCGGCACTGCTGAGT
  CTGACGGGAAGCGGCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024301 unedited
  NNGGTCCTTACCCCCGCCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCT
  ATATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAG
  CGGCCGCGAATTCGGCACGAGGCTTCGGCCCCATGCGGCTCACCCGCTGCCAGGCTGCCC
  TGGCGGCCGCCATCACCCTCAACCTTCTGGTCCTCTTCTATGTCTCGTGGCTGCAGCACC
  AGCCTAGGAATTCCCGGGCCCGGGGGCCCCGTCGTGCCTCTGCTGCCGGCCCCCGTGTCA
  CCGTCCTGGTGCGGGAGTTCGAGGCATTTGACAACGCGGTGCCCGAGCTGGTAGACTCCT
  TCCTGCAGCAAGACCCAGCCCAGCCCGTGGTGGTGGCAGCCGACACGCTCCCCTACCCGC
  CCCTGGCCCTGCCCCGCATCCCCAACGTGCGTCTGGCGCTGCTCCAGCCCGCCCTGGACC
  GGCCAGCCGCAGCCTCGCGCCCGGAGACCTACGTGGCCACCGAGTTTGTGGCCCTAGTAC
  CTGATGGGGCGCGGGCTGAGGCACCTGGCCTGCTGGAGCGCATGGTGGAGGCGCTCCGCG
  CAGGAAGCGCACGTCTGGTGGCCGCCCCGGTTGCCACGGCCAACCCTGCCAGGTGCCTGG
  CCCTGAACGTCAGCCTGCGAGAGTGGACCGCCCGCTATGGCGCAGCCCCCGCCGNGCCCC
  GCTGCGACGCCCTGGACGGAGATGCTGTGGTGCTCCTGCGCGCCCGCGACCTCTTAACCT
  CTCGGCGCCCCTGCCCGCCGGGGGCACCAGCCTCTTCTGCAGACGCCCTCGCGCTGGCCG
  TGCACCTGCTGACTTGACTTCCCCGGGNGCGCAGCCCCGTGGCCCGCCCACCCGCTGAAG
  GCTGACGCAGACCGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024301 unedited
  NCCGTATACTATGNACCGCGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTTAAAA
  TGGAATAATTTAAGCTTTATTACTAACATACAGATAATAGTATCTGTATATATTTATAAA
  TAAATATACACATATTGGGAATACATGCCCAAATACCATTTATTCTTAGGTGCATAATCA
  AATAAGGAAGGCTGCCATTGCTTTCACCTGCTAAGTGGGTCTCCAAGCCCCTCTTGGGGA
  CCATGACTAGAGAGGGGCATAGTGGGTAAGACAATTGTGGGCTCCAGAGCCTGACCACCT
  GGGTTCAAGCCCCCGATCTGCCCCTTCCTAAGCTGCCTGACCTTGAGCAAGTGACTTCAC
  GATTCTGGCCTCAGCTTTTTCATCTGTGTAATGAAAATAATAGCAGCTGTCACTTCAAAG
  GGCTGAGGTGAGGGACGCATTAATTGATCCAGGTGAAACACTGAGAATGGTGCCTGGTAC
  CCAGTAAGTGCTCAGTGAGTGTGAGCTATGGTTATTGTCTACACTGCCGTGCAAGTGGGG
  AATTAGGTGCAGACAGCTGAAATAATGAGGTATTAGTCATGAGTGGAGCTGCGATTCAAA
  AATCGGGTCTTTTTCTTTTTTAAAAATAGAGACAGGGTCTTGGCATGTTGCCGAAGCTGG
  TCTCAGACTCCTGGGCTCAAACGATCCTCCCACCTCGGCCTCTCAAAATGCTGGGAGTAC
  ACGCGTGAGACCCCGCGCCCGGGCCAAAACCCGGTTTTTGACCACCAACCCCCCTCGGAG
  CTCTGAGCATTTTCTTCTCTGGAAGACGAAAAAAATTCTTTTCAATCTTGATATAACAAT
  TAAGAAAACATGCCGGGCACAGGGCTCTTCCAAGAAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_024301
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024301.2, NP_077277.1
• RefSeq Size: 3422 bp
• RefSeq ORF: 1488 bp
• Locus ID: 79147
• UniProt ID: Q9H9S5
• Cytogenetics: 19q13.32
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a protein which is targeted to the medial Golgi apparatus and is necessary for posttranslational modification of dystroglycan. Mutations in this gene have been associated with congenital muscular dystrophy, cognitive disability, and cerebellar cysts. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Oct 2008]
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.