QTRT1 (NM_031209) Human Untagged Clone

CAT#: SC111450

QTRT1 (untagged)-Human queuine tRNA-ribosyltransferase 1 (QTRT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: QTRT1
• Synonyms: FP3235; TGT; TGUT
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_031209, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGGAGCAGCTACCCAGGCTTCCCTGGAGTCGGCCCCACGGATCATGCGGCTGGTGGCCGAATGCA
  GCCGCTCCAGGGCCCGGGCAGGCGAGCTGTGGCTGCCGCATGGGACAGTGGCCACTCCTGTGTTCATGCC
  AGTGGGCACGCAGGCCACCATGAAGGGCATCACGACCGAACAGCTGGACGCTCTGGGTTGCCGCATCTGC
  CTGGGCAATACCTACCATCTGGGTCTAAGGCCGGGACCCGAGCTGATCCAGAAAGCCAACGGTCTCCACG
  GCTTCATGAATTGGCCTCATAATCTGCTAACGGACAGCGGCGGTTTCCAGATGGTGTCGCTGGTGTCTCT
  GTCCGAGGTGACGGAGGAGGGCGTCCGCTTCCGCTCCCCCTACGACGGCAATGAGACCCTGCTGAGCCCG
  GAGAAATCCGTGCAGATCCAGAATGCGCTGGGCTCGGACATCATCATGCAGCTGGACGACGTGGTTAGCA
  GTACTGTGACTGGGCCACGTGTGGAGGAGGCCATGTACAGGTCAATCCGCTGGCTGGACCGGTGCATTGC
  AGCCCATCAGCGGCCGGACAAGCAGAACCTCTTCGCCATTATCCAGGGTGGGCTGGACGCAGATCTCCGG
  GCCACCTGCCTTGAAGAGATGACCAAGCGAGACGTGCCTGGCTTCGCCATCGGGGGCCTGAGCGGGGGTG
  AGAGCAAGTCGCAGTTCTGGCGGATGGTGGCGCTGAGCACCTCTCGGCTGCCGAAGGACAAGCCCCGATA
  TCTGATGGGGGTTGGCTATGCCACTGATCTGGTAGTCTGCGTGGCTCTTGGATGTGACATGTTCGACTGC
  GTCTTCCCCACACGGACAGCGCGCTTTGGCTCTGCCCTGGTGCCCACTGGGAACCTGCAGTTGAGGAAGA
  AGGTGTTTGAGAAGGACTTCGGCCCCATAGACCCGGAGTGCACCTGCCCCACGTGCCAAAAGCACAGCCG
  CGCCTTCCTGCACGCACTGCTGCACAGTGACAACACGGCCGCGCTGCACCACCTCACGGTCCACAACATC
  GCCTACCAGCTGCAGCTCATGAGCGCCGTCCGCACCAGCATCGTGGAGAAGCGCTTCCCGGACTTCGTGC
  GGGACTTCATGGGCGCCATGTACGGGGATCCCACCCTCTGTCCCACCTGGGCCACTGACGCTCTGGCCTC
  TGTGGGAATCACACTGGGCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031209 unedited
  NGGTGCACATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGAGTCAAG
  ATGGCGGGAGCAGCTACCCAGGCTTCCCTGGAGTCGGCCCCACGGATCATGCGGCTGGTG
  GCCGAATGCAGCCGCTCCAGGGCCCGGGCAGGCGAGCTGTGGCTGCCGCATGGGACAGTG
  GCCACTCCTGTGTTCATGCCAGTGGGCACGCAGGCCACCATGAAGGGCATCACGACCGAA
  CAGCTGGACGCTCTGGGTTGCCGCATCTGCCTGGGCAATACCTACCATCTGGGTCTAAGG
  CCGGGACCCGAGCTGATCCAGAAAGCCAACGGTCTCCACGGCTTCATGAATTGGCCTCAT
  AATCTGCTAACGGACAGCGGCGGTTTCCAGATGGTGTCGCTGGTGTCTCTGTCCGAGGTG
  ACGGAGGAGGGCGTCCGCTTCCGCTCCCCCTACGACGGCAATGAGACCCTGCTGAGCCCG
  GAGAAATCCGTGCAGATCCAGAATGCGCTGGGCTCGGACATCATCATGCAGCTGGACGAC
  GTGGTTAGCAGTACTGTGACTGGGCCACGTGTGGAGGAGGCCATGTACAGGTCAATCCGC
  TGGCTGGACCGGTGCATTGCAGCCCATCAGCGGCCGGACAAGCAGAACCTCTTCGCCATT
  ATCCAGGGTGGGCTGGACGCAGATCTCCGGGCCACCTGCCTTGAAGAGATGACCAAGCGA
  GACGTGCCTGGCTTCGCCATCGGGGGCCTGAGCGGNGGTGAGAGCAAGTCGCAGTTCTGG
  CGGATGGTGGCGCTGAGCACCTCTCGGCTGCCGAAGGACAAGCCCGATATCTGAGGGGGT
  TGGGTATGTTGTGGATAGGGAAGCCGNACCCTCCTGTGGGAAGTGGTATCCTGGGGACCC
  CCTACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031209 unedited
  AAAAGAGCTACGTGNANAAAAAACCCNCTCCTTAGGTAATGCCTTGCACTATNCTTACCA
  ACACCCTACCGCTCCCTTGCCTTTTCCTCCCTCNCCTTCTCCCAATGGCCGAGGTCAGCT
  CCAGTGTGTATTCCCACAGAGGCCAGGGCGTTATTGGCCCATGTGGGACAGAGGGTGGGA
  TCCTCGTTCATGGTGCCCATGAAGTCCCCGTACGAAGTCCGGGTAGCGCTTCTCCACCAT
  GCTGTTTTCTACGGCGCTCATTACCTGCAGGGCGGGGGCGATGTTTTGCACCTTGAGGTG
  GTGCATCGCCGTTCGTGTTTTCACTGTTTCATCATTTCCTTTCAGGAAGGCCCGGCTTGT
  GCTTTTCGGCCCTCTGTTGCCAGTGTCCTTCCTGGTTTTATGGGGCTCTAAATCCTTTTT
  TAAACCCCTTCTTTTTTCACCTTCTGTTCCCTTGTGGCCCCACGGCACTATCCAATTCGC
  CCTTTCCCTGTGGGAAAACGCCCTCCACCATGTACATCCCAGACCTCTGCTTACTTCTCT
  ATTATTTGCTTATCTTGTTGTTGTTTCTTGACCGCAGTGGCCGATAACCTTATGTGCTTC
  CTTTGGCCCCTCCCAACCCTTCACATTGCCCCACTATAGTTCCCTTTTCCCCTCACATTG
  CCCCCTTCTGGATAACCATCGTTTTCCTATTCTCCTTAGATCATCCCTGTCTTCCCTTCC
  TCCATATGCTCCTTCCCCACTCTGTGTAGGGTGTCTCCATGCATTCACTCCCACAGGTCG
  GGCCCTAGCTTTCCTATCACACATACCCAATCTTCTAACAACTCCCCCCTCGCCTTTCCC
  TTCCCTTAGTCCTTTGCCCCCTTCGCTTACCTTGTATTTTCTTCCCCCCCTTCTGTTCTT
  CACGCAAAATCCCCCTTCTTTCTTCGCATATTTTATGAGTGAGCTCTGCCTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_031209
• Insert Size: 1580 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031209.1, NP_112486.1
• RefSeq Size: 1313 bp
• RefSeq ORF: 1212 bp
• Locus ID: 81890
• UniProt ID: Q9BXR0
• Cytogenetics: 19p13.2
• Domains: TGT
• Gene Summary: This gene encodes the catalytic subunit of tRNA-guanine transglycosylase. tRNA-guanine transglycosylase is a heterodimeric enzyme complex that plays a critical role in tRNA modification by synthesizing the 7-deazaguanosine queuosine, which is found in tRNAs that code for asparagine, aspartic acid, histidine and tyrosine. A pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq, Feb 2012]