PCDHA4 (NM_031500) Human Untagged Clone

CAT#: SC110571

PCDHA4 (untagged)-Human protocadherin alpha 4 (PCDHA4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCDHA4
• Synonyms: CNR1; CNRN1; CRNR1; PCDH-ALPHA4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_031500, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGTTTTCCTGGGGAAGCGGCCAGGAATCCCGGCGTCTGCTGCTCTTACTTCTTCTCCTCGCAGCCT
  GGGAGGCAGGGAACGGTCAGCTCCACTACTCGGTCTCCGAGGAGGCCAAACACGGCACCTTCGTGGGCCG
  CATCGCGCAGGACCTGGGACTGGAGCTGGCGGAGCTGGTGCCGCGCCTGTTCCGGGTGGCGTCCAAGGGC
  CGCGGAGGCCTTCTGGAGGTAAATCTGCAGAATGGCATTTTGTTTGTGAATTCTCGGATCGACCGGGAGG
  AGCTGTGCCGGCGGAGCGCGGAGTGCAGCATCCACCTGGAGGTGATCGTAGACAGGCCGCTGCAGGTTTT
  CCATGTGGACGTGGAGGTGAGGGACATTAACGATAACCCGCCGGTGTTCCCAGCAACACAAAAGAACCTG
  TCCATCGCGGAATCCAGGCCGCTTGACTCTCGGTTTCCACTAGAGGGCGCCTCGGATGCAGATATCGGGG
  AGAACGCCCTGCTCACTTACAGACTGAGCCCAAATGAATACTTTTCTCTGGAAAAACCACCTGATGACGA
  GCTGGTAAAAGGTCTTGGGCTTATATTACGGAAATCTTTAGACAGAGAAGAAGCTCCGGAGATTTTTTTA
  GTGCTCACAGCCACTGATGGAGGCAAACCCGAGTTGACTGGCACCGTTCAGTTACTCATCACAGTACTGG
  ATGCCAATGACAATGCCCCAGCTTTTGACAGAACCATTTATAAGGTGAGATTACTAGAAAATGTTCCTAA
  TGGAACATTGGTAATTAAACTTAACGCCTCAGATTTAGACGAAGGATTGAATGGGGACATTGTTTATTCA
  TTCTCAAATGATATTTCGCCAAATGTGAAATCCAAGTTTCACATAGATCCAATTACTGGACAAATTATTG
  TAAAGGGATATATTGACTTTGAAGAAAGCAAATCCTATGAAATTATTGTAGAGGGCATTGATAAGGGACA
  GCTCCCACTTTCTGGCCATTGTAGAGTTATTGTGGAAGTAGAAGACAACAACGATAATGTCCCAGATTTG
  GAATTCAAGTCTTTATCACTTCCAATTAGAGAGGACGCTCCACTGGGTACAGTCATCGCCCTGATCAGCG
  TGTCCGACAAAGACATGGGTGTCAATGGGCTGGTCACCTGCTCCTTGACGTCCCACGTCCCCTTCAAGCT
  GGTGTCCACCTTCAAGAATTACTACTCGTTGGTGCTGGACAGTGCCCTGGACCGCGAGAGCGTGTCAGCC
  TATGAGCTGGTGGTGACCGCGCGAGACGGGGGCTCGCCTTCGCTGTGGGCCACGGCCAGTGTTTCTGTGG
  AGGTGGCTGATGTGAACGACAACGCTCCGGCGTTCGCGCAGCCCGAGTACACAGTGTTCGTGAAGGAGAA
  CAACCCGCCGGGCTGCCACATCTTCACTGTGTCTGCGTGGGACGCGGACGCGCAGGAGAACGCGCTGGTG
  TCCTACTCGCTGGTAGAGCGGCGGGTAGGGGAGCGCGCGCTGTCGAGCTACGTTTCGGTGCATGCGGAGA
  GCGGCAAGGTGTACGCGCTGCAGCCGCTGGACCACGAGGAGCTAGAGCTGCTGCAGTTTCAGGTGACCGC
  TCGCGATGCCGGCGTGCCACCTCTGGGCAGCAACGTGACGCTGCAGGTGTTCGTGCTGGACGAAAACGAC
  AACGCGCCAGCACTGCTAGCGCCTCGGGCGGGTGGCACTGGTGGCGCAGTGAGCGAGCTGGTGCCATGGT
  CGGTGGGTGTGGGCCACGTGGTGGCAAAGGTGCGCGCGGTGGATGCTGACTCGGGCTACAACGCGTGGCT
  TTCGTACGAGCTGCAGCCGGGGACTGGTGGCGCGCGCATCCCGTTCCGCGTGGGGCTGTACACTGGCGAG
  ATCAGCACAACGCGTGCCCTGGACGAAACGGACGCTCCGCGCCACCGCCTACTGGTACTGGTGAAGGACC
  ACGGCGAGCCCGCGCTGACGGCCACGGCCACTGTGCTGGTGTCACTTGTGGAGAGTGGACAGGCGCCAAA
  GGCCTCCTCACGGGCGTTGGTGGGCGCTGTGGGTCCCGATGCTGCGCTGGTGGATGTCAACGTATACCTG
  ATCATTGCCATCTGCGCGGTGTCCAGCCTTTTGGTGCTCACGCTGCTGCTGTACACCGCGCTGCGGTGCT
  CTGCGCTGCCCACCGAGGGCGCGTGCGCTCCGGGCAAGCCCACGCTGGTGTGCTCCAGTGCGGTGGGGAG
  CTGGTCATACTCGCAGCAGAGGAGGCCGAGGGTGTGCTCTGGTGAGGGCCCACCCAAGACCGACCTCATG
  GCCTTCAGCCCCAGTTTACCTGACTCTAGGGACAGAGAAGATCAGCTGCAGACAACTGAGGAATCCTTTG
  CAAAGGTTAGTGTATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031500 unedited
  GTTGTTCAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGAGACT
  GAATATGGAGGATGCAGCTGCACTTGACTGACCGATTAAAAGATTTCCCTTGACTTTGAG
  AAACGATATTTAATCAGAACAAAATACTGTGCACTAAAGATGGAGTTTTCCTGGGGAAGC
  GGCCAGGAATCCCGGCGTCTGCTGCTCTTACTTCTTCTCCTCGCAGCCTGGGAGGCAGGG
  AACGGTCAGCTCCACTACTCGGTCTCCGAGGAGGCCAAACACGGCACCTTCGTGGGCCGC
  ATCGCGCAGGACCTGGGACTGGATCTGGCGGAGCTGGTGCCGCGCCTGTTCCGGGTGGCG
  TCCAAAAGACACCGGGACCTTCTGGAGGTAAATCTGCAGAATGGCATTTTGTTTGTGAAT
  TCTCGGATCGACCGCGAGGAGCTGTGCCGGCGGAGCGCGGAGTGCAGCATCCACCTGGAG
  GTGATCGTGGACAGGCCGCTGCAGGTTTTCCATGTGGACGTGGAGGTGAGGGACATTAAC
  GATAACCCGCCGGTGTTCCCAGCAACACAAAAGAACCTGTCCATCGCGGAATCCAGGCCG
  CTTGACTCTCGGTTTCCACTAGAGGGCGCCTCGGATGCAGATATCGGNGAGAACGCCCTG
  CTCACTTACAGACTGAGCCCAAATGAATACTTTTCTCTGGGAAAACCATCTGATGACGAG
  CTGGTAAAAGGTCTTGGGCTTATATTACGGAAATCTTTAGACAGAGAAGAAGCTCCGGAG
  ATTTTTTTAGTGCTCACAGCCACTGATGGAGGCAAACCCGAGTTGACTGGCCCCGTTCAG
  TTACTCATCACAGAACTGGATGCCAATGACAATGCCCCACTTTTGACAGAACATTTTTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031500 unedited
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTTGGTCTCCTCCTTTTT
  GCCGAAGGTTATGAAGTCACTTTTGTCAATTTGGCTGTTAGTAGGCTCCTGCCGGATGGA
  GATGATTGCAGGAGATCCTGGGATAATGAATTTGTCGGGCAACTCACCGGGACCGGATTG
  TTTGGGGTTGCCTGGTCCGTATTTAAAGGTCCAGCTGTTGCTGTTGACACCCGCACCGAC
  TGGAGGGGACACTTCTCCTGCCTCTGGTTCTGGTGTTGCACTGGATACTGTTGGCCACTG
  CTGATCAGGCCCTCCTGGACCAGCCCGTAGAATGCCAGCCTCCTCTAGGTGCACAGAGCT
  GTGCATGCCTGCTCTCAGGGAGGCAGAGTAACGCCAGTCAGGGTTGGGCTGTCGTGGCTT
  TGCAAAGGATTCCTCAGTTGTCTGCAGCTGATCTTCTCTGTCCCTAGAGTCAGGTAAACT
  GGGGCTGAAGGCCATGAGGTCGGTCTTGGGTGGGCCCTCACCAGAGCACACCCTCGGCCT
  CCTCTGCTGCGAGTATGACCAGCTCCCCACCGCACTGGAGCACACCAGCGTGGGCTTGCC
  CGGAGCGCACGCGCCCTCGGTGGGCAGCGCAGAGCACCGCAGCGCGGTGTACAGCAGCAG
  CGTGAGCACCAAAAGGCTGGACACCGCGCAGATGGCAATGATCAGGTATACGTTGACATC
  CACCAGCGCAGCATCGGGACCCACAGCGCCCACCAACGCCCGTGAGGAGGCCTTTGGCGC
  CTGTCCACTCTTCACAGTGACACCAGCACAGTGGCCGTGCCCGTCATCGCGGCCTCGCCG
  TGGTCCTTCACAGTACCATTAGCCGTGGCGCCGAACGTCCGTTTCGTCCAGGCAGCGTTG
  TGCTGATCTCGCAGTGTACACCCCAGCGTACGGGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_031500
• Insert Size: 2870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031500.1, NP_113688.1
• RefSeq Size: 2498 bp
• RefSeq ORF: 2397 bp
• Locus ID: 56144
• UniProt ID: Q9UN74
• Cytogenetics: 5q31.3
• Domains: CA
• Protein Families: Transmembrane
• Gene Summary: This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) utilizes the large, first exon then continues into the downstream intron 1 sequence before terminating. This one-exon transcript encodes a shorter isoform (2), compared to isoform 1. This short variant is represented based on data in PMID:10380929.