GABA A Receptor gamma 2 (GABRG2) (NM_000816) Human Untagged Clone

CAT#: SC109261

GABRG2 (untagged)-Human gamma-aminobutyric acid (GABA) A receptor, gamma 2 (GABRG2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GABA A Receptor gamma 2
• Synonyms: CAE2; DEE74; ECA2; EIEE74; FEB8; GEFSP3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000816, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGTTCGCCAAATATATGGAGCACAGGAAGCTCAGTCTACTCGACTCCTGTATTTTCACAGAAAATGA
  CGGTGTGGATTCTGCTCCTGCTGTCGCTCTACCCTGGCTTCACTAGCCAGAAATCTGATGATGACTATGA
  AGATTATGCTTCTAACAAAACATGGGTCTTGACTCCAAAAGTTCCTGAGGGTGATGTCACTGTCATCTTA
  AACAACCTGCTGGAAGGATATGACAATAAACTTCGGCCTGATATAGGAGTGAAGCCAACGTTAATTCACA
  CAGACATGTATGTGAATAGCATTGGTCCAGTGAACGCTATCAATATGGAATACACTATTGATATATTTTT
  TGCGCAAACGTGGTATGACAGACGTTTGAAATTTAACAGCACCATTAAAGTCCTCCGATTGAACAGCAAC
  ATGGTGGGGAAAATCTGGATTCCAGACACTTTCTTCAGAAATTCCAAAAAAGCTGATGCACACTGGATCA
  CCACCCCCAACAGGATGCTGAGAATTTGGAATGATGGTCGAGTGCTCTACACCCTAAGGTTGACAATTGA
  TGCTGAGTGCCAATTACAATTGCACAACTTTCCAATGGATGAACACTCCTGCCCCTTGGAGTTCTCCAGT
  TATGGCTATCCACGTGAAGAAATTGTTTATCAATGGAAGCGAAGTTCTGTTGAAGTGGGCGACACAAGAT
  CCTGGAGGCTTTATCAATTCTCATTTGTTGGTCTAAGAAATACCACCGAAGTAGTGAAGACAACTTCCGG
  AGATTATGTGGTCATGTCTGTCTACTTTGATCTGAGCAGAAGAATGGGATACTTTACCATCCAGACCTAT
  ATCCCCTGCACACTCATTGTCGTCCTATCCTGGGTGTCTTTCTGGATCAATAAGGATGCTGTTCCAGCCA
  GAACATCTTTAGGTATCACCACTGTCCTGACAATGACCACCCTCAGCACCATTGCCCGGAAATCGCTCCC
  CAAGGTCTCCTATGTCACAGCGATGGATCTCTTTGTATCTGTTTGTTTCATCTTTGTCTTCTCTGCTCTG
  GTGGAGTATGGCACCTTGCATTATTTTGTCAGCAACCGGAAACCAAGCAAGGACAAAGATAAAAAGAAGA
  AAAACCCTGCCCCTACCATTGATATCCGCCCAAGATCAGCAACCATTCAAATGAATAATGCTACACACCT
  TCAAGAGAGAGATGAAGAGTACGGCTATGAGTGTCTGGACGGCAAGGACTGTGCCAGTTTTTTCTGCTGT
  TTTGAAGATTGTCGAACAGGAGCTTGGAGACATGGGAGGATACATATCCGCATTGCCAAAATGGACTCCT
  ATGCTCGGATCTTCTTCCCCACTGCCTTCTGCCTGTTTAATCTGGTCTATTGGGTCTCCTACCTCTACCT
  GTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000816 unedited
  NNGGTCAGAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGGTATCT
  GCAAGCGTTTTTGCTGATCTTATCTCTGCCCCCTNGATATTAATTCCCTAATCTGGTAGC
  AATCCATCTCCCCAGTGAAGGACCTACTAGAGGCAGGTGGGGGGAGCCACCATCAGATCA
  TAAGCATAAGAATAATACAAAGGGGAGGGATTCTTCTGCAACCAAGAGGCAAGAGGCGAG
  AGAAGGAAAAAAAAAAAAGCGATGAGTTCGCCAAATATATGGAGCACAGGAAGCTCAGTC
  TACTCGACTCCTGTATTTTCACAGAAAATGACGGTGTGGATTCTGCTCCTGCTGTCGCTC
  TACCCTGGCTTCACTAGCCAGAAATCTGATGATGACTATGAAGATTATGCTTCTAACAAA
  ACATGGGTCTTGACTCCAAAAGTTCCTGAGGGTGATGTCACTGTCATCTTAAACAACCTG
  CTGGAAGGATATGACAATAAACTTCGGCCTGATATAGGAGTGAAGCCAACGTTAATTCAC
  ACAGACATGTATGTGAATAGCATTGGTCCAGTGAACGCTATCAATATGGAATACACTATT
  GATATATTTTTTGCGCAAACGTGGTATGACAGACGTTTGAAATTTAACAGCACCATTAAA
  GTCCTCCGATTGAACAGCAACATGGTGGGGAAAATCTGGATTCCAGACACTTTCTTCAGA
  AATTCCAAAAAAGCTGATGCACACTGGGATCACCACCCCCAACAGGATGCTNGAGATTTG
  GAATGGATGGTCGAGTGCTCTACACCCTAAGGTTGACAATTGATGCTGAGTGCCAATTAC
  AATTGCACAAACTTTCCATGGATGAACACTNNCTGCCCCTGGNANTNCTCCAGGTATGGC
  TATCCACGTGAAGAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000816 unedited
  ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGATAACTTCAGTCCATT
  TATTTTGGATCTAAAGAGATGCATGCACTAAACTTGAAGCATTCTAAGTTATTGAAATAT
  TTACATCATTGTACATGAATGTGGAGGAAATTTTCTTCCTATCTTGAGATAAAGGTGTAA
  TAGATTTCTGGACTAGGCTTTCATATTTAAAGTGAGTTATGTTTTTTCTTAAATGAGGGA
  GGTATTCTATTGGGATCAAATTTATCACAACTTGACCCGGAGTGAACATAGGGTACAGTT
  TTGGACCTTTTCTATCTAATTGCATTATGAATCTAGTAAGGATACCACTACTGTAAATAG
  TCAGGGCTTTCACTGTTTTATTTGCTGTAGCTGAAAAATATCTTATTTGGTTTGAATGTT
  CTTGCTCTAATTTTACTTTGCCTTGCAGGTTTGAAACACAGCAAACATCGAAGTTCAAAG
  TACACTAACCAAAGGAGGGCTGGGCACAAACAATATGACATTATAAAATTTACCAGGTTT
  GGACATAGAAACAGATTTCAGATCATTATCAACCACATAGAGGATTATTTAAGTGGACTT
  AGAACAGACATCTCTCCATGAGACTCAGTGAATAAGAACCCTATCAGTAAAACCCATACC
  TTCTCACAGGTAGAGGTAGGAGACCCCATTGACCAATTAAACAGCCCAAGGCAGTGGGGA
  AAAGATCCGACCTAAGAGTCCATTTTGGCAAGCCGATATGGATCCTCCATGTTTTCCAAC
  TCCTGGTCGACAATCTTAAAACACCAAAAAAAACTGCCAAGTCTTTGCGTCCAAACCTCT
  AACCGGCCTCTCAATTTTTTTTGGAGGGGGTAACATTATTTTTGAATGGTGCGAACTCTG
  GCGGTATAATGGTAGGCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000816
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000816.2, NP_000807.2
• RefSeq Size: 2370 bp
• RefSeq ORF: 1404 bp
• Locus ID: 2566
• UniProt ID: P18507
• Cytogenetics: 5q34
• Domains: Neur_chan_memb, Neur_chan_LBD
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammlian brain, where it acts at GABA-A receptors, which are ligand-gated chloride channels. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene have been associated with epilepsy and febrile seizures. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks two alternate in-frame exons, compared to variant 3. It encodes isoform 2 which is shorter than isoform 3.