RNF34 (NM_025126) Human Untagged Clone

CAT#: SC108787

RNF34 (untagged)-Human ring finger protein 34 (RNF34), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RNF34
• Synonyms: CARP-1; CARP1; hRFI; RFI; RIF; RIFF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_025126 edited
  GAATTCGGCACGAGGCGGCAGTGTGAGGAGCTGCTATGGTGCTGAGTTTCCTGGTAGAGC
  CGGCCGAGCTGAGGCGGTCGCGGCCATGAAGGCGGGTGCCACGTCTATGTGGGCTTCGTG
  CTGTGGGCTGCTGAATGAAGTCATGGGAACTGGAGCTGTCAGGGGCCAGCAGTCAGCATT
  TGCAGGAGCCACCGGTCCATTCAGATTTACACCAAACCCTGAGTTTTCCACCTACCCACC
  AGCAGCTACGGAAGGGCCCAACATAGTTTGTAAAGCCTGTGGGCTTTCATTTTCAGTCTT
  TAGAAAGAAGCATGTTTGCTGTGACTGCAAGAAGGATTTTTGCTCCGTTTGTTCAGTCTT
  ACAAGAAAATCTCCGTAGATGTTCTACTTGTCACTTATTACAAGAGACAGCATTTCAGCG
  CCCTCAGTTAATGCGACTGAAGGTGAAGGACCTGCGGCAGTATCTCATTCTGAGAAATAT
  ACCCATAGATACTTGTCGTGAGAAAGAAGACTTGGTGGATCTAGTACTGTGCCATCATGG
  ACTAGGCTCTGAGGACGACATGGACACAAGCAGTCTGAATTCTTCAAGGTCCCAGACTTC
  TAGCTTTTTTACACGTTCGTTTTTTTCAAACTATACAGCCCCCTCTGCTACTATGTCTTC
  GTTTCAGGGAGAGCTTATGGATGGAGACCAAACATCCAGATCTGGAGTGCCGGCACAGGT
  ACAAAGTGAAATCACTTCAGCAAACACAGAAGATGATGATGACGACGATGATGAGGATGA
  TGATGATGAAGAAGAAAACGCAGAGGATCGGAACCCCGGGCTCTCCAAGGAGAGAGTGAG
  AGCTTCACTGTCTGACTTGTCAAGCCTTGATGATGTGGAAGGAATGAGCGTGCGCCAGCT
  GAAGGAAATTCTGGCTCGGAATTTTGTCAACTATTCTGGCTGTTGTGAAAAATGGGAACT
  GGTAGAGAAAGTAAACCGGTTATACAAAGAGAATGAAGAAAACCAAAAGTCCTATGGCGA
  GCGGCTGCAGCTGCAGGATGAGGAAGACGACAGCCTGTGTCGCATCTGCATGGATGCCGT
  CATCGACTGTGTCCTACTGGAGTGTGGGCACATGGTTACCTGCACCAAGTGCGGCAAGCG
  CATGAGTGAGTGTCCCATCTGCCGGCAGTATGTGGTGCGAGCCGTGCACGTGTTCAAGTC
  CTGAAACAGGCTCCCCTCACCAGGACAGTCACCCCCAAACTTGACCCCCAACATTTCAAT
  GCACAGAAGGGACTGGAAAGTTATGTTCAAAGGCTGAAGCTATTTTAAAACATTATTTTG
  ACTACTAAGTGGGGACAGAAAGATCCATCCTGAGTTGTGGAAACATTGGTCCATGCCGTG
  AGCCTGTCTGCCTGTGGACACGTGAGCTTCCCGGGCTCAGCTGGGCTTTATCACACATCC
  CGTGAACACTCATTGAAGTCAGCCTGTTTGCGCCATGTGGGCATCAGCCACTGCTGTCTT
  GGGAGGACACTTATCCTGTTCTCTTATTTCCCCTTCATCCTATTTTTAACTTAAACTGCT
  CAGATGTTTGAAACTTCTGTCCTCTTTGGATGAGATCAGTGTCCACAAGTGGCCGACATG
  GAACATGCTGAGCAGTGGCTCCTCTGAATGTTCACTTTATTAGTCATGTATATTTTAAAT
  GCTAACATTTGATGAATXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACT
  CGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_025126 unedited
  AACGTACAAAATTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCGGCAGT
  GTGAGGAGCTGCTATGGTGCTGAGTTTCCTGGTAGAGCCGGCCGAGCTGAGGCGGTCGCG
  GCCATGAAGGCGGGTGCCACGTCTATGTGGGCTTCGTGCTGTGGGCTGCTGAATGAAGTC
  ATGGGAACTGGAGCTGTCAGGGGCCAGCAGTCAGCATTTGCAGGAGCCACCGGTCCATTC
  AGATTTACACCAAACCCTGAGTTTTCCACCTACCCACCAGCAGCTACGGAAGGGCCCAAC
  ATAGTTTGTAAAGCCTGTGGGCTTTCATTTTCAGTCTTTAGAAAGAAGCATGTTTGCTGT
  GACTGCAAGAAGGATTTTTGCTCCGTTTGTTCAGTCTTACAAGAAAATCTCCGTAGATGT
  TCTACTTGTCACTTATTACAAGAGACAGCATTTCAGCGCCCTCAGTTAATGCGACTGAAG
  GTGAAGGACCTGCGGCAGTATCTCATTCTGAGAAATATACCCATAGATACTTGTCGTGAG
  AAAGAAGACTTGGTGGATCTAGTACTGTGCCATCATGGACTAGGCTCTGAGGACGACATG
  GACACAAGCAGTCTGAATTCTTCAAGGTCCCAGACTTCTAGCTTTTTTACACGTTCGTTT
  TTTTCAAACTATACAGCCCCCTCTGCTACTATGTCTTCGTTTCAGGGAGAGCTTATGGAT
  GGAGACCAAACATCCAGATCTGGAGTGCCGGCACAGGTACAAAGTGAAAATCACTTCAGC
  AACACAGAAGATGATGATGACGACGATGATGAGGATGATGATGATGAAGAAGAAACGCAG
  AGGATCGGAACCCCGGNCTCTCCAGGAGAGAGTGAGAGCTTCACTGTCTGACTTTGTCAG
  CCCTTGATGATGTGAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_025126 unedited
  CTGTTTGTCCCCCCCCCGATTACAAATCGTCTACGCGGCCCACCACTGCAAAAACACCTC
  CACTATACACAACAATGTACACTGATCACACATTAATAAGGCAATTTACCACACACACTT
  CTAATAAAAGAAGCTACATGCAGCCCTTGCTATAAAAAGCTATACCTGAACAAAAATGGA
  CATGTGGAGTCTAAGGCCCTGGCAGTCGACTAGAGAATGTCAGTTGTTCCCAATTATGTT
  CAAATGCAGAAACAGCAACAATGTGGAAACTTACATTCATCAAATGTTACCATCTAAAAT
  ATACATGACTACTAAAGCGAACATTTACAGGCGCCACTGCTCATCATGTTCCACGTTGGC
  CACTTGTGGACACTGATCTCATCCAAAGAGGACAGAAGTTTCAAACATCTGAGCAGCTTA
  AGTTAAAAATAGGACGAAGGGGAAATAAGAGAACAGGATAAGTGTCCTCCCAAGACAGCA
  CTGGCTGATGCCCACATGGTGCAAACAGGCTGACTTCCATGAGTGTTCACCGCATGCGTG
  ACAAAGCCTATCTGAGCCCGGGAAGCTCACGTGCTCACAGGCAGACAGGCTGACGGCATG
  GACCAACGCTTCCACCACTTACGCATGGATCCTTCTGTCCCCACTTATTACGTAAAATAA
  TGTCTTACCACATCCTAATCCCCTGCCCCTAACTCTCCANTCCCTTCTGCGCATCGAAAT
  GCTGGGGGCCATCGCCGCGTCGCCTCTTCTGTTGAGCCAGCCCGCCCCTGACTTCCCTCT
  TCCCGGCCCGCACACACATTGCGGCCAACGGGAACTTACTCACGCGCCTGCGCATTGGCG
  TACCAACTTTGTCCCATTCTCCCATGGATCAGTCCCTTCCGTATTCTGCCTAGTGGACAC
  GGCTGCCCTCTTCTACTCCGTCCCTGCAACGTCGCCCCAAACTTCGGTTCTTCTCCCCTC
  CGACAGCCTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_025126
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025126.2, NP_079402.2
• RefSeq Size: 2039 bp
• RefSeq ORF: 1119 bp
• Locus ID: 80196
• UniProt ID: Q969K3
• Cytogenetics: 12q24.31
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]
  Transcript Variant: This variant (2) lacks an exon in the 5' coding region which results in the use of an alternate translational start codon, compared to variant 1. The resulting isoform (2) lacks one amino acid in the N-terminus, compared isoform 1.