COPG (COPG1) (NM_016128) Human Untagged Clone

CAT#: SC108411

COPG1 (untagged)-Human coatomer protein complex, subunit gamma (COPG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COPG
• Synonyms: COPG
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108411 sequence for NM_016128 edited (data generated by NextGen Sequencing)
  ATGTTGAAGAAATTCGACAAGAAGGATGAGGAGTCAGGTGGAGGCTCCAACCCATTCCAG
  CACCTTGAGAAGAGTGCGGTACTCCAGGAGGCCCGTGTATTTAATGAAACTCCCATCAAC
  CCTCGGAAATGTGCCCACATCCTCACCAAGATTCTTTATCTCATAAACCAGGGGGAGCAC
  CTGGGGACCACGGAAGCGACCGAGGCCTTCTTTGCCATGACCAAGCTCTTTCAGTCCAAT
  GATCCCACACTCCGTCGGATGTGCTACTTGACCATCAAGGAGATGTCTTGCATTGCAGAG
  GATGTCATCATTGTCACCAGCAGCCTAACAAAAGACATGACTGGGAAAGAAGACAACTAC
  CGGGGCCCGGCCGTGCGAGCCCTCTGCCAGATCACTGATAGCACCATGCTGCAGGCTATT
  GAGCGCTACATGAAACAAGCCATTGTGGACAAGGTGCCCAGTGTCTCCAGCTCTGCCCTC
  GTGTCTTCCTTGCACCTGCTGAAGTGCAGCTTTGACGTGGTCAAGCGCTGGGTGAATGAG
  GCTCAGGAGGCAGCATCCAGTGATAACATCATGGTCCAGTACCACGCACTAGGGCTCCTG
  TACCATGTGCGTAAGAATGACCGCCTAGCCGTCAATAAGATGATCAGCAAGGTCACACGG
  CATGGCCTTAAGTCTCCCTTTGCCTACTGCATGATGATCCGGGTGGCCAGCAAGCAGCTG
  GAAGAGGAGGATGGCAGCCGTGACAGCCCACTGTTTGACTTCATCGAGAGCTGCTTGCGC
  AACAAGCACGAGATGGTGGTGTATGAAGCCGCCTCGGCCATCGTCAATCTGCCAGGCTGC
  AGTGCCAAAGAGCTGGCCCCGGCTGTGTCAGTGCTCCAGCTTTTCTGCAGCTCACCCAAG
  GCTGCTCTCCGCTATGCTGCTGTTCGTACCCTCAATAAGGTTGCCATGAAGCATCCGTCA
  GCTGTGACAGCTTGTAATCTGGATCTGGAGAACCTGGTCACAGATTCAAACCGCAGCATT
  GCCACGCTGGCCATCACCACCCTCCTTAAGACGGGCAGCGAGAGCAGCATCGACCGCCTC
  ATGAAGCAGATCTCCTCCTTCATGTCAGAAATCTCGGATGAATTCAAGGTGGTGGTTGTC
  CAGGCCATCAGTGCCCTGTGTCAGAAATATCCTCGCAAACACGCCGTCCTTATGAACTTC
  CTGTTCACCATGCTGCGGGAAGAGGGTGGCTTTGAGTATAAGCGCGCTATCGTGGACTGC
  ATCATCAGCATCATTGAAGAGAACTCAGAGAGCAAGGAGACAGGGCTGTCACATCTGTGC
  GAGTTCATCGAGGACTGCGAGTTCACAGTGCTGGCCACCCGTATTCTACATCTCCTGGGC
  CAGGAGGGGCCCAAGACCACCAATCCCTCAAAGTACATCCGCTTCATCTATAACCGAGTG
  GTCTTGGAGCATGAGGAGGTCCGGGCAGGTGCTGTGAGTGCTCTGGCGAAGTTTGGAGCC
  CAGAATGAAGAGATGTTACCCAGTATCTTGGTGTTGCTGAAGAGGTGTGTGATGGATGAT
  GACAATGAAGTAAGGGACCGAGCCACCTTCTACCTAAATGTCCTGGAGCAGAAGCAGAAG
  GCCCTTAATGCAGGCTATATCCTAAATGGTCTGACTGTGTCCATCCCTGGTCTGGAGAGG
  GCTCTGCAGCAGTACACTCTAGAACCATCAGAAAAACCTTTTGACCTCAAGTCTGTGCCC
  CTGGCCACGGCGCCCATGGCAGAGCAGAGAACAGAAAGTACCCCCATCACAGCAGTCAAA
  CAGCCTGAGAAAGTGGCAGCTACCAGGCAGGAGATCTTCCAGGAGCAGTTGGCAGCAGTG
  CCAGAGTTCCGCGGTCTTGGGCCCCTCTTCAAGTCCTCGCCTGAGCCCGTGGCCCTCACC
  GAGTCAGAGACGGAGTATGTCATCCGCTGCACCAAACACACCTTCACCAACCACATGGTT
  TTTCAGTTTGACTGCACAAACACACTCAATGACCAGACCTTGGAGAATGTCACAGTGCAG
  ATGGAGCCCACTGAGGCCTATGAGGTGCTCTGTTACGTGCCTGCCCGGAGCCTGCCCTAC
  AACCAGCCCGGGACCTGCTACACACTGGTGGCACTGCCCAAAGAAGACCCCACAGCTGTG
  GCCTGCACATTCAGCTGCATGATGAAGTTCACTGTCAAGGACTGTGATCCCACCACTGGG
  GAGACTGATGACGAAGGCTATGAGGATGAGTATGTGCTGGAAGATCTGGAAGTTACTGTA
  GCTGATCACATTCAAAAGGTCATGAAACTGAACTTCGAAGCAGCCTGGGATGAGGTAGGG
  GATGAATTTGAGAAGGAGGAAACGTTCACCTTGTCTACCATCAAGACACTTGAAGAGGCT
  GTGGGTAATATTGTGAAGTTCTTGGGAATGCACCCTTGTGAGAGGTCAGACAAAGTGCCG
  GATAACAAGAACACCCACACGTTGCTCCTGGCTGGTGTGTTCCGGGGTGGTCATGACATC
  CTGGTGCGCTCCCGGCTGCTGCTTTTGGACACAGTGACAATGCAGGTGACAGCCAGAAGT
  TTGGAGGAGCTGCCAGTAGACATCATCTTGGCATCTGTGGGATAA
  
  Clone variation with respect to NM_016128.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016128 unedited
  NTGTTCGAATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCGTT
  GCTGCATTGCGCCCCACCGACTCCACTATGTTGAAGAAATTCGACAAGAAGGATGAGGAG
  TCAGGTGGAGGCTCCAACCCATTCCAGCACCTTGAGAAGAGTGCGGTACTCCAGGAGGCC
  CGTGTATTTAATGAAACTCCCATCAACCCTCGGAAATGTGCCCACATCCTCACCAAGATT
  CTTTATCTCATAAACCAGGGGGAGCACCTGGGGACCACGGAAGCGACCGAGGCCTTCTTT
  GCCATGACCAAGCTCTTTCAGTCCAATGATCCCACACTCCGTCGGATGTGCTACTTGACC
  ATCAAGGAGATGTCTTGCATTGCAGAGGATGTCATCATTGTCACCAGCAGCCTAACAAAA
  GACATGACTGGGAAAGAAGACAACTACCGGGGCCCGGCCGTGCGAGCCCTCTGCCAGATC
  ACTGATAGCACCATGCTGCAGGCTATTGAGCGCTACATGAAACAAGCCATTGTGGACAAG
  GTGCCCAGTGTCTCCAGCTCTGCCCTCGTGTCTTCCTTGCACCTGCTGAAGTGCAGCTTT
  GACGTGGTCAAGCGCTGGGTGAATGAGGCTCAGGAGGCAGCATCCAGTGATAACATCATG
  GTCCAGTACCACGCACTAGGGCTCCTGTACCATGTGCGTAAGAATGACCGCCTAGCCGTC
  AATAAGATGATCAGCAAGGTCACACGGCATGGCCTTAAGTCTCCCTTTGCCTACTGCATG
  ATGATCCGGGTGGCCAGCAAGCAGCTGGAAGAGGAGGATGGCAGCCGTGACAGCCCACTG
  TTTGACTTCATCGAGAGCTGCTTGCGCAACAGCACGAGATGGTGGTGTATGAAGCCGNNC
  TCGCCATCGTCAATCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016128 unedited
  TTGGACCGGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATAAATACATG
  CTGATTTATTACAGGGATAAGATGGTTTCTTGGGGGATAGATTCAAGAGGAGTTGAGAAT
  GTTTTATTCATTTACAATGTCCCTTTCCTGGAAGGGTGGACAGCAAGATTTAGGACAAGC
  TAAAATCATCCCCTATTTAAAAAAAAAAAAAAAAAAAGTCACCAGCAAGTATTCCCGGGT
  GGGAGGTGGGAGCAGAATAAAAAAAAATCTGCAATGATTCCTAATTGTTTTTCAATACAG
  AAGCTTGGGAAGGGGTTTCTGCCAGTTTCATGAGGAAGGCACAACTTCCAGGTAGTGTTG
  GGGAAGGGTATGAGGTCCTATGCAGGCTGGCCTCTTATCCCACAGATGCCAAGATGATGT
  CTACTGGCAGCTCCTCCAAACTTCTGGCTGTCACCTGCATTGTCACTGTGTCCAAAAGCA
  GCAGCCGGGAGCGCACCAGGATGTCATGACCACCCCGGAACACACCAGCCAGGAGCAACG
  TGTGGGTGTTCTTGTTATCCGGCACTTTGTCTGACCTCTACAAGGGTGCATTCCCAAGAA
  CTTCACAATATTACCCACAGCCTCTTCAAGTGTCTTGATGGTAGACAAGGTGAACGTTTC
  CTCCTTCTCAAATTCATCCCCTACCTCATCCCAGGCTGCTTCGAAGTTCAGTTTCATGAC
  CTTTTGAATGTGATCAGCTACAGTAACTTNCAGATCTTCCAGCACATACTCATCCTCATA
  GCCTTCGTCATCAGTCTCCCCAGTGGTGGGATCACAGTCCTTGACAGTGAACTTCATCAT
  GCAGCTGAATGTGCAGGCCACAGCTGTGGGGGTCTTCTTTGGGCANTGCCACAGTGTGTA
  NCAGGTCCCGGNNCTGGTGTAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016128
• Insert Size: 3180 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016128.3, NP_057212.1
• RefSeq Size: 3114 bp
• RefSeq ORF: 2625 bp
• Locus ID: 22820
• UniProt ID: Q9Y678
• Cytogenetics: 3q21.3
• Domains: Adaptin_N
• Protein Families: Druggable Genome
• Gene Summary: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis (By similarity).[UniProtKB/Swiss-Prot Function]