Profilin 2 (PFN2) (NM_053024) Human Untagged Clone

CAT#: SC107927

PFN2 (untagged)-Human profilin 2 (PFN2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Profilin 2
• Synonyms: D3S1319E; PFL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107927 sequence for NM_053024 edited (data generated by NextGen Sequencing)
  ATGGCCGGTTGGCAGAGCTACGTGGATAACCTGATGTGCGATGGCTGCTGCCAGGAGGCC
  GCCATTGTCGGCTACTGCGACGCCAAATACGTCTGGGCAGCCACGGCCGGGGGCGTCTTT
  CAGAGCATTACGCCAATAGAAATAGATATGATTGTAGGAAAAGACCGGGAAGGTTTCTTT
  ACCAACGGTTTGACTCTTGGCGCGAAGAAATGCTCAGTGATCAGAGATAGTCTATACGTC
  GATGGTGACTGCACAATGGACATCCGGACAAAGAGTCAAGGTGGGGAGCCAACATACAAT
  GTGGCTGTCGGCAGAGCTGGTAGAGTCTTGGTCTTTGTAATGGGAAAAGAAGGGGTCCAT
  GGAGGCGGATTGAATAAGAAGGCATACTCAATGGCAAAATACTTGAGAGACTCTGGGTTC
  TAG
  
  Clone variation with respect to NM_053024.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_053024 unedited
  CACGAATTTGTNAATACGCACTTCACTTATANNGGCGGCCGCGNAATTCGGCACGAGGGC
  GCCGCCGCCGCCCGCTACCGCCGCCGCCGCCGCTGCGCCTGCTGCTCCTCGCCGTCCGCG
  CTGCAGTGCGAAGGGCTCGAAGATGGCCGGTTGGCAGAGCTACGTGGATAACCTGATGTG
  CGATGGCTGCTGCCAGGAGGCCGCCATTGTCGGCTCGTGCGACGCCAAATACGTCTGGGC
  AGCCACGGCCGGGGGCGTCTTTCAGAGCATTACGCCAATAGAAATAGATATGATTGTAGG
  AAAAGACCGGGAAGGTTTCTTTACCAACGGTTTGACTCTTGGCGCGAAGAAATGCTCAGC
  GATCAGAGATAGTCTATACGTCGATGGTGACTGCACAATGGACATCCGGACAAAGAGTCA
  AGGTGGGGAGCCAACATACAATGTGGCTGTCGGCAGAGCTGGTAGAGCCTTGGTCTTTGT
  CCTGGGAAAAGAAGGGGTCCATGGAGGCGGATTGAATAAGAAGGCATACTCAATGGCAAA
  ATACTTGAGAGACTCTGGGTTCTAGCTGCTAGGCCGACTGCCCCGTATTAGGGGAAAACT
  GCTCTTAAACTTTCCTAGCTATAAACTCAAGTCTTAATTCTGGAAATCTATTAACCACTG
  CCGGCTGATGGGGTATGAACCTGTGTCTCCTTTGTTCCCTCTGCTGGCGGGGAAAGGTGT
  CCTTCTTTCTGCCCTCCCCCCCCAAATAACTCTGTTAACTTTGTTTGGCTCCCCTGGGAC
  CCCACCATTGGTTTAGTCATGGGAAACGAAGGGGAGTCCGGCGGCCCACTTACCACAAAA
  CATAGCACCCCCTTTTTCCCGCAGAGGTCTATTGTACACAACCTTCCCCATTTACCTAAA
  CTGATTTCCTCCCACCCTATTATGCGCCACGCTCCCCCTGTCGCGCCGCGTATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_053024
• Insert Size: 2290 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053024.2, NP_444252.1
• RefSeq Size: 2272 bp
• RefSeq ORF: 423 bp
• Locus ID: 5217
• UniProt ID: P35080
• Cytogenetics: 3q25.1
• Domains: PROF
• Protein Families: Druggable Genome
• Protein Pathways: Regulation of actin cytoskeleton
• Gene Summary: The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes isoform a.