PRMT1 (NM_198319) Human Untagged Clone

CAT#: SC107794

PRMT1 (untagged)-Human protein arginine methyltransferase 1 (PRMT1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRMT1
• Synonyms: 6720434D09Rik; ANM1; arginine N-methyltransferase 1; AW214366; HCP1; heterogeneous nuclear ribonucleoproteins methyltransferase-like 2; HRMT1L2; Hrmt1l2; IR1B4; Mrmt1; OTTMUSP00000022387; protein arginine N-methyltransferase 1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_198319 edited
  ATGGAGAATTTTGTAGCCACCTTGGCTAATGGGATGAGCCTCCAGCCGCCTCTTGAAGAA
  GTAACCCCCTTTGCCCTTCCCTGTGTCTGCCCCCATTTTCCTTCCCCTCCCCTCCCCAGC
  TGTGGGCTGAGCTAGAGACGGGGTCAGAGAGACTGGAGAGATGGTAGGCGTGGCTGAGGT
  GTCCTGTGGCCAGGCGGAAAGCAGTGAGAAGCCCAACGCTGAGGACATGACATCCAAAGA
  TTACTACTTTGACTCCTACGCACACTTTGGCATCCACGAGGAGATGCTGAAGGACGAGGT
  GCGCACCCTCACTTACCGCAACTCCATGTTTCATAACCGGCACCTCTTCAAGGACAAGGT
  GGTGCTGGACGTCGGCTCGGGCACCGGCATCCTCTGCATGTTTGCTGCCAAGGCCGGGGC
  CCGCAAGGTCATCGGGATCGAGTGTTCCAGTATCTCTGATTATGCGGTGAAGATCGTCAA
  AGCCAACAAGTTAGACCACGTGGTGACCATCATCAAGGGGAAGGTGGAGGAGGTGGAGCT
  CCCAGTGGAGAAGGTGGACATCATCATCAGCGAGTGGATGGGCTACTGCCTCTTCTACGA
  GTCCATGCTCAACACCGTGCTCTATGCCCGGGACAAGTGGCTGGCGCCCGATGGCCTCAT
  CTTCCCAGACCGGGCCACGCTGTATGTGACGGCCATCGAGGACCGGCAGTACAAAGACTA
  CAAGATCCACTGGTGGGAGAACGTGTATGGCTTCGACATGTCTTGCATCAAAGATGTGGC
  CATTAAGGAGCCCCTAGTGGATGTCGTGGACCCCAAACAGCTGGTCACCAACGCCTGCCT
  CATAAAGGAGGTGGACATCTATACCGTCAAGGTGGAAGACCTGACCTTCACCTCCCCGTT
  CTGCCTGCAAGTGAAGCGGAATGACTACGTGCACGCCCTGGTGGCCTACTTCAACATCGA
  GTTCACACGCTGCCACAAGAGGACCGGCTTCTCCACCAGCCCCGAGTCCCCGTACACGCA
  CTGGAAGCAGACGGTGTTCTACATGGAGGACTACCTGACCGTGAAGACGGGCGAGGAGAT
  CTTCGGCACCATCGGCATGCGGCCCAACGCCAAGAACAACCGGGACCTGGACTTCACCAT
  CGACCTGGACTTCAAGGGCCAGCTGTGCGAGCTGTCCTGCTCCACCGACTACCGGATGCG
  CTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_198319 unedited
  GGTTANGAATAGTATACGACTCCTATAGGCGGCCGCGAAATTCGCACGAGGGCGGCCGGT
  AGAGGAGTAGGTGCGGGTGAAGATGGCGGCAGCCGAGGCCGCGAACTGCATCATGGAGAA
  TTTTGTAGCCACCTTGGCTAATGGGATGAGCCTCCAGCCGCCTCTTGAAGAAGTAACCCC
  CTTTGCCCTTCCCTGTGTCTGCCCCCATTTTCCTTCCCCTCCCCTCCCCAGCTGTGGGCT
  GAGCTAGAGACGGGGTCAGAGAGACTGGAGAGATGGTAGGCGTGGCTGAGGTGTCCTGTG
  GCCAGGCGGAAAGCAGTGAGAAGCCCAACGCTGAGGACATGACATCCAAAGATTACTACT
  TTGACTCCTACGCACACTTTGGCATCCACGAGGAGATGCTGAAGGACGAGGTGCGCACCC
  TCACTTACCGCAACTCCATGTTTCATAACCGGCACCTCTTCAAGGACAAGGTGGTGCTGG
  ACGTCGGCTCGGGCACCGGCATCCTCTGCATGTTTGCTGCCAAGGCCGGGGCCCGCAAGG
  TCATCGGGATCGAGTGTTCCAGTATCTCTGATTATGCGGTGAAGATCGTCAAAGCCAACA
  AGTTAGACCACGTGGTGACCATCATCANAGNGAANGTGGAGGANGTGGAGCTCCCAGTGG
  AGAAGGTGGACATCATCATCAGCGAGTGGATGGGCTACTGCCTCTTCTACGAGTCCATGC
  TCAACACCCGTGCTCTATGCCCCGGNACAAGTTGGCTGCGCCCGATGGCCTCATCTTCCC
  AGACCGGGCCACGCTGTATGTGACNGCCATCGAGGACCCGCAGTACAAAGACTACCAGAT
  CCACTGTTGGGAGAACGTGTATGCCTNCGACATGTCTTGCATCAAAGATGTGGCCACTAA
  AGAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_198319 unedited
  CCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTCCCAGCTGAGGATTTATTGGCGTA
  AATGCAACCATATAAAAACATAAGTTATGAAAAACACAGTCACGATGTGCCCTCCCCATC
  CCCCCAGCCCAGGCCCCTAAAACCCCCTTCTGCGGGAGGGAGGGAGAGGAAGGGGGAGCC
  CCGAAACCGCCTAGGAACGCTCAGCCCCTGGGCTCGTGCAGGGCGGGAGAGCCGGGCCTC
  AGCGCATCCGGTAGTCGGTGGAGCAGGACAGCTCGCACAGCTGGCCCTTGAAGTCCAGGT
  CGATGGTGAAGTCCAGGTCCCGGTTGTTCTTGGCGTTGGGCCGCATGCCGATGGTGCCGA
  AGATCTCCTCGCCCGTCTTCACGGTCAGGTAGTCCTCCATGTAGAACACCGTCTGCTTCC
  AGTGCGTGTACGGGGACTCGGGGCTGGTGGAGAAGCCGGTCCTCTTGTGGCAGCGTGTGA
  ACTCGATGTTGAAGTAGGCCACCAGGGCGTGCACGTAGTCATTCCGCTTCACTTGCAGGC
  AGAACGGGGAGGTGAAGGTCAGGTCTTCCACCTTGACGGTATAGATGTCCACCTCCTTTA
  TGAGGCAGGCGTTGGTGACCAGCTGTTTGGGGTCCACGACATCCACTAGGGGGCTCCTTA
  ATGGCCACATCTTTGATGCAAGACATGTCGAAGCCATACACGTTCTCCCCCAGTGGGATC
  TGTAGTCTTTGTACTGCNCGTCCTCGATGGCCGTACATACAGCGTGGCCCGGTCTGGGAA
  GAGAGGCCATCCGGCGCCAGCCACTTGTCCCGGCATAGAGCACGGTGTGAGCTGGACTCG
  TAAAAAGCAGTACCCATCCCTCCTGAGATGATGTCACCTTCTCACTGGAGCTCACCTNCT
  CCACTTCCCTGAGATGGCCCACGGGNNCTACTGTGGTTTGCGATCTACCGATATA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_198319
• Insert Size: 1550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198319.1, NP_938075.1
• RefSeq Size: 1435 bp
• RefSeq ORF: 1044 bp
• Locus ID: 3276
• Cytogenetics: 19q13.33
• Gene Summary: This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]