PUS7L (NM_031292) Human Untagged Clone

CAT#: SC107702

PUS7L (untagged)-Human pseudouridylate synthase 7 homolog (S. cerevisiae)-like (PUS7L), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PUS7L
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107702 sequence for NM_031292 edited (data generated by NextGen Sequencing)
  ATGGAAGAAGATACAGATTATAGAATCAGGTTTAGTTCTTTGTGTTTCTTTAATGATCAC
  GTTGGATTTCATGGCACTATAAAAAGCTCACCAAGTGACTTTATTGTTATTGAAATTGAT
  GAACAGGGACAGTTAGTTAATAAGACCATCGATGAGCCTATTTTCAAGATTAGTGAAATA
  CAACTTGAGCCAAATAATTTTCCCAAAAAACCAAAACTAGATCTTCAAAATCTGTCCTTA
  GAAGATGGAAGAAACCAAGAAGTTCATACTTTGATTAAGTACACTGATGGTGACCAAAAT
  CATCAGTCTGGTTCAGAAAAGGAAGATACTATCGTTGATGGAACTTCCAAATGTGAAGAA
  AAAGCTGATGTTTTAAGCTCCTTTTTGGATGAAAAAACTCATGAGTTACTGAATAATTTT
  GCCTGTGATGTAAGAGAGAAGTGGCTTTCTAAAACAGAGCTAATTGGACTACCTCCTGAA
  TTCTCAATAGGCAGAATCCTTGACAAAAACCAGAGGGCTAGTTTACACAGTGCCATTAGG
  CAGAAATTTCCATTTTTAGTAACTGTAGGAAAAAACAGTGAAATTGTTGTAAAACCAAAT
  CTTGAATATAAAGAACTTTGTCATTTGGTATCTGAAGAGGAAGCATTTGACTTTTTTAAA
  TATTTGGATGCAAAGAAAGAAAATTCCAAATTTACCTTTAAACCTGATACAAACAAAGAC
  CACAGAAAAGCTGTCCACCATTTTGTCAACAAAAAGTTTGGAAACCTTGTGGAAACCAAA
  TCTTTTTCTAAAATGAATTGCAGTGCTGGTAATCCGAATGTGGTGGTAACAGTAAGATTT
  CGGGAAAAAGCACACAAACGTGGGAAAAGGCCTCTTTCTGAATGCCAAGAAGGAAAAGTT
  ATATATACAGCTTTTACCCTACGAAAGGAAAACCTGGAAATGTTTGAAGCGATTGGTTTT
  TTAGCTATCAAACTTGGTGTTATTCCTTCGGATTTTAGTTATGCAGGCCTTAAAGACAAG
  AAAGCCATCACCTATCAAGCAATGGTTGTTAGAAAAGTGACTCCAGAGAGGTTGAAAAAT
  ATTGAAAAAGAAATTGAAAAGAAAAGAATGAATGTCTTTAATATTCGGTCTGTAGATGAT
  TCCCTGAGACTTGGTCAGCTCAAAGGAAATCACTTTGATATTGTCATTAGAAATTTAAAA
  AAACAAATAAATGATTCTGCAAACCTGAGGGAGAGAATTATGGAAGCAATAGAAAATGTT
  AAGAAAAAAGGCTTTGTGAATTACTATGGACCACAGAGATTTGGGAAGGGAAGGAAAGTT
  CACACAGACCAAATTGGACTAGCTTTGCTGAAGAATGAAATGATGAAAGCCATAAAATTG
  TTTCTTACACCAGAAGACTTGGATGATCCTGTAAATAGAGCAAAGAAGTATTTTCTTCAA
  ACTGAGGATGCTAAAGGCACACTTTCATTGATGCCTGAATTCAAAGTGCGTGAGAGAGCA
  TTGTTGGAGGCATTGCACCGCTTTGGCATGACCGAGGAAGGTTGTATCCAGGCATGGTTC
  TCTTTACCCCATTCCATGCGCATATTCTATGTTCACGCATATACCAGCAAAATTTGGAAT
  GAGGCAGTATCTTACAGACTTGAAACCTATGGAGCAAGAGTAGTGCAGGGTGATTTGGTC
  TGTTTGGATGAAGACATTGATGACGAGAATTTCCCAAATAGTAAAATTCACCTGGTAACT
  GAAGAGGAGGGATCAGCTAATATGTATGCAATACATCAGGTGGTTCTTCCAGTACTTGGA
  TACAATATTCAGTACCCGAAGAACAAAGTAGGGCAGTGGTACCATGACATACTTAGCAGA
  GATGGACTACAGACATGTAGGTTTAAAGTACCTACTCTGAAACTGAATATACCAGGTTGC
  TATAGACAGATTTTGAAACATCCCTGTAATCTCTCATACCAACTAATGGAAGATCATGAC
  ATTGATGTCAAAACGAAAGGTTCCCACATTGATGAAACAGCTTTGTCTCTTTTGATCTCT
  TTTGATCTTGATGCTTCATGCTATGCTACCGTTTGTCTGAAGGAAATAATGAAGCATGAC
  GTTTAA
  
  Clone variation with respect to NM_031292.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031292 unedited
  TATAGGGGCGGCCGCGATTCGGCACGAGGTAGACGGGGTCGAGTTTCTTGCGCGATGGCA
  CTGTTATAGAAGAATGGAAGAAGATACAGATTATAGAATCAGGTTTAGTTCTTTGTGTTT
  CTTTAATGATCACGTTGGATTTCATGGCACTATAAAAAGCTCACCAAGTGACTTTATTGT
  TATTGAAATTGATGAACAGGGACAGTTAGTTAATAAGACCATCGATGAGCCTATTTTCAA
  GATTAGTGAAATACAACTTGAGCCAAATAATTTTCCCAAAAAACCAAAACTAGATCTTCA
  AAATCTGTCCTTAGAAGATGGAAGAAACCAAGAAGTTCATACTTTGATTAAGTACACTGA
  TGGTGACCAAAATCATCAGTCTGGTTCAGAAAAGGAAGATACTATCGTTGATGGAACTTC
  CAAATGTGAAGAAAAAGCTGATGTTTTAAGCTCCTTTTTGGATGAAAAAACTCATGAGTT
  ACTGAATAATTTTGCCTGTGATGTAAGAGAGAAGTGGCTTTCTAAAACAGAGCTAATTGG
  ACTACCTCCTGAATTCTCAATAGGCAGAATCCTTGACAAAAACCAGAGGGCTAGTTTACA
  CAGTGCCATTAGGCAGAAATTTCCATTTTTAGTAACTGTAGGAAAAAACAGTGAAATTGT
  TGTAAAACCAAATCTTGATATAAAGAACTTTGTCATTTGGTATCTGAAGAGGAAGCTTTT
  GACTTTTTTAAATATTTGGATGCAAAGAAAGAANATTCCANATTTACCTTTTAACCCTGN
  TACAACCAAAGACCCAGAANAAGCTTGCCACCCATTTTTGTCAACAAAAGTTTGGGAAAC
  CTTGGGGAAACCAAAATCTTTTCTAAATGAAATTGCAGGGCTGGTATCCGAAATGGGGGG
  GGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031292 unedited
  NTTAGCTCTGGACCCGCGGCCGCAATCTAGGATCGAGNTTTTTTTTTTTTTTTTTTTTTT
  GGGGATTGAGAATTATTTATTGCCAAAACATATAACAAAAAAAACTTTTTAGTATTAAAA
  AAAATCCAAATACCCCATCATCTTAACAGGAAGGGATTTTCATTTATTTGCTTTTCTCTT
  CCAAACTTGCATCTGCAGACACTTACTTAACAATAACTAAGTGCCAGGCACTTTTTAAAG
  GTGCTTTACATATATTAACTTAAAAAATTCTCACAGTAATCCTATGAGGTAGGCAATGTT
  AACATGCCCATTTTACAGATGTTGAAACTGAAGCCCATGAGTTTAAATAACTTTCCCAAG
  GCTGCAGTGCTATACGTGGCAGTGCCAGGGTTCAAACTGGGTTAGTTTGTCTCCAATATC
  TGTGCTTTTAATCACTATGCTAACATGTCTCTTATTTGTGAAAGATGAATTCAGAGTATT
  ATTGCAGTTGTAACATATGTGTTCTACTTTTAGGGGGCAACATTTTCTCATGAAACTAAA
  TTGGTCATTGCTTTTTTAAATAACTACATATTTTACCATCAAGTTGATACACATCATTTT
  ACTTACTTTAGTTACGTAATGCTGGACTTTGAAAACTGTTTTTAGTGTCTGTATATGAAA
  ATATTGCAATAAGCAAATTTTGCATTGCATATAGCTTCTCTTCCTTCTTTTGGATTTTAT
  TTCCTAATGCTAACATTTCCCAAAATGTNTAAACTGGCTTAAGTATATATATCACATTAC
  TGATATATAAAGCTCCATACTGCTTTTTAATTTCATCATTATATTCTACCAACATGAAAG
  AAAGCTCAGACTTCCTAAACATACCTGTCTACTGCAATCATTCTCCCCACACACCCTATT
  TACTGATTAAAAGGTGAAGGAGATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_031292
• Insert Size: 4200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031292.2, NP_112582.2
• RefSeq Size: 3959 bp
• RefSeq ORF: 2106 bp
• Locus ID: 83448
• UniProt ID: Q9H0K6
• Cytogenetics: 12q12
• Domains: UPF0024
• Gene Summary: Pseudouridylate synthase that catalyzes pseudouridylation of RNAs.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same isoform (a).