MRPL52 (NM_181305) Human Untagged Clone

CAT#: SC107204

MRPL52 (untagged)-Human mitochondrial ribosomal protein L52 (MRPL52), nuclear gene encoding mitochondrial protein, transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRPL52
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_181305 edited
  ATGAAAGGCCAGCTTCGAAGAAAAGCTGAAAGGGAGACGTTTGCAAGACGAGTTGTACTG
  CTGTCACAGGAAATGGACGCTGGATTACAAGCATGGCAGCTCAGGCAGCAGAAGTTGCAG
  GAAGAACAAAGGAAGCAGGAAAATGCTCTTAAACCCAAAGGGGCTTCACTGAAGAGCCCA
  CTTCCAAGTCAATAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_181305 unedited
  TGGCTGCCAACCCCTCCGGCTACGGGCCCCTTACCGAGCTCCCAGACTGGTCATATGCGG
  ATGGCCGCCCTGCTCCCCCAATGAAAGGCCAGCTTCGAAGAAAAGCTGAAAGGGAGACGT
  TTGCAAGACGAGTTGTACTGCTGTCACAGGAAATGGACGCTGGATTACAAGCATGGCAGC
  TCAGGCAGCAGAAGTTGCAGGAAGAACAAAGGAAGCAGGAAAATGCTCTTAAACCCAAAG
  GGGCTTCACTGAAGAGCCCACTTCCAAGTCAATAAAAAGCAACTCCTGCCTCCCTTCCTC
  NNAAAAAAAAAAANNAANNNAAANNNNNNNAAANNNANANNNNANNNANANNNNNAAAAN
  ANNANNNNNNNNNNNNNNANAANNNNANNNAANNANANNNNNNANAANNAAAAAAAAAAN
  AANCNNCTCTTTTAATTGGGGGNCGGGNTTTNTTTTTTTTNTTAANAAAACCCNGGGGGG
  ATTCCTTGGACCCCNCCCCAAGGGCCTTTNTGGGCCTGGAAATTGCCCCNCCNNGGNCCC
  NCCCCTTTTGGCTAAAAAAAAAAAAGGGCCATTTTTTTGGGNGNAAAGGGGGCCNTTTTA
  AATTGGGGGGGGGGGGGGGGTTTTTTNNAAAAGGGGGCCNNTTTNAAAAAAACCCTGGGG
  GCCGNGGGGGGTTTTTGGGAACCCCTNGGGGGGGGGGGCAANTTTGGGTCCNGGAATCCC
  CCCTCNGGGGTNAANAAANTTTNCCCCNCCCCCCCNGGGGGGGGGGTTNCCCCCCCCCAC
  CCCCCCTTTTTTTTTTTTTTTTTGGAAAAAAGGGGGTTTCCATTTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_181305
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181305.1, NP_851822.1
• RefSeq Size: 1223 bp
• RefSeq ORF: 195 bp
• Locus ID: 122704
• UniProt ID: Q86TS9
• Cytogenetics: 14q11.2
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which has no bacterial homolog. Multiple transcript variants encoding different protein isoforms were identified through sequence analysis. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (5) contains an additional segment, compared to variant 1, which leads to the use of a downstream start codon. The predicted protein (isoform d) is shorter than isoform a. The predicted ORF of this transcript has not been experimentally confirmed. Variants 4, 5 and 6 encode the same isoform.