ch TOG (CKAP5) (BC017856) Human Untagged Clone

CAT#: SC106049

CKAP5 (untagged)-Homo sapiens, clone IMAGE:4699215

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CKAP5
• Synonyms: ch-TOG; CHTOG; colonic and hepatic tumor over-expressed; colonic and hepatic tumor over-expressed protein; cytoskeleton associated protein 5; FLJ35359; KIAA0097; MSPS; TOG; TOGp
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC017856, the custom clone sequence may differ by one or more nucleotides
  
  
  GGCGGCCCAAGCGGTTTCAAACGGCTTAGAGCAGGCCGCTTGGTTCTGACCCAGCTGAGGAAATACTCTT
  AATTCTAAGGAAAACCTGGAAGCACAATGGGAGATGACAGTGAGTGGTTGAAACTGCCAGTTGATCAGAA
  ATGTGAACACAAGCTGTGGAAAGCAAGGTTAAGTGGGTATGAAGAGGCCCTGAAGATCTTCCAGAAAATA
  AAGGATGAAAAGAGCCCAGAGTGGTCCAAATTTTTAGGATTGATCAAAAAATTTGTCACTGATTCCAATG
  CAGTGGTTCAATTGAAAGGATTAGAAGCTGCACTTGTTTATGTTGAAAATGCCCATGTAGCAGGAAAAAC
  CACAGGAGAAGTTGTGTCAGGTGTTGTAAGTAAGGTGTTCAATCAACCTAAAGCTAAAGCCAAGGAGCTG
  GGCATAGAGATCTGTCTTATGTACATAGAGATTGAGAAAGGAGAGGCTGTTCAAGAAGAGCTCCTGAAAG
  GCTTGGACAATAAGAATCCCAAGATCATAGTGGCCTGTATAGAGACACTGAGGAAAGCCTTAAGTGAATT
  TGGTTCCAAAATCATCTTGCTTAAGCCAATTATCAAAGTGTTGCCAAAACTCTTTGAGTCTCGAGAGAAG
  GCTGTTCGAGATGAAGCCAAACTAATTGCTGTGGAGATTTACAGATGGATTCGGGATGCTCTGAGACCCC
  CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC017856 unedited
  NNGGGGTGCACATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGGCGGG
  GTGTGCGGCGGCCCAAGCGGTTTCAAACGGCTTAGAGCAGGCCGCTTGGTTCTGACCCAG
  CTGAGGAAATACTCTTAATTCTAAGGAAAACCTGGAAGCACAATGGGAGATGACAGTGAG
  TGGTTGAAACTGCCAGTTGATCAGAAATGTGAACACAAGCTGTGGAAAGCAAGGTTAAGT
  GGGTATGAAGAGGCCCTGAAGATCTTCCAGAAAATAAAGGATGAAAAGAGCCCAGAGTGG
  TCCAAATTTTTAGGATTGATCAAAAAATTTGTCACTGATTCCAATGCAGTGGTTCAATTG
  AAAGGATTAGAAGCTGCACTTGTTTATGTTGAAAATGCCCATGTAGCAGGAAAAACCACA
  GGAGAAGTTGTGTCAGGTGTTGTAAGTAAGGTGTTCAATCAACCTAAAGCTAAAGCCAAG
  GAGCTGGGCATAGAGATCTGTCTTATGTACATAGAGATTGAGAAAGGAGAGGCTGTTCAA
  GAAGAGCTCCTGANAGGCTTGGACAATAAGAATCCCAAGATCATAGTGGCCTGTATAGAG
  ACACTGAGGAAAGCCTTAAGTGAATTTGGTTCCAAAATCATCTTGCTTAAGCCAATTATC
  AAAGTGTTGCCAAAACTCTTTGAGTCTCGAGAGAAGGCTGTTCGAGATGAAGCCAAACTA
  ATTGCTGTGGGAGATTACAGATGGATTCNGGATGCTCTGAGACCCCCATTACAAATATAA
  ACTCTGTTCAGTTGAAAAAAACTAGAAGAAGAATGGGTCANACTGCCAACAAGTGCTCCT
  AGACCTACTCCGATTCTTCCGTTCCACAAGAACTAGAAGCTAAATTGGACCACACAGTCT
  GCTGGTGGAGATGCTGN
  
• Restriction Sites: NotI-NotI
• ACCN: BC017856
• Insert Size: 7000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC017856.1, AAH17856.1
• RefSeq Size: 731 bp
• RefSeq ORF: 731 bp
• Locus ID: 9793
• Cytogenetics: 11p11.2
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]