TBC1D2 (AY026527) Human Untagged Clone

CAT#: SC105232

(untagged)-Human prostate antigen PARIS-1 mRNA, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBC1D2
• Synonyms: PARIS-1; PARIS1; TBC1D2A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC105232 sequence for AY026527 edited (data generated by NextGen Sequencing)
  ATGGAGGGCGCTGGGGAGAACGCCCCGGAGTCCAGCTCCTCTGCCCCTGGGTCCGAAGAG
  TCTGCCAGGGATCCACAGGTGCCGCCTCCGGAGGAAGAATCGGGGGACTGCGCCCGGTCC
  CTGGAGGCGGTCCCCAAGAAACTCTGTGGGTATTTAAGTAAGTTCGGCGGCAAAGGGCCC
  ATCCGGGGCTGGAAATCCCGCTGGTTCTTCTACGACGAAAGGAAATGTCAGCTGTATTAC
  TCGCGGACCGCTCAGGATGCCAATCCCTTGGACAGCATCGACCTCTCCAGTGCAGTGTTT
  GACTGTAAGGCGGACGCTGAGGAGGGGATCTTCGAAATCAAGACTCCCAGCCGGGTTATT
  ACCCTGAAGGCCGCCACCAAGCAAGCGATGCTGTACTGGCTGCAGCAGCTGCAGATGAAG
  CGCTGGGAATTCCACAACAGCCCGCCGGCACCTCCTGCCACCCCTGATGCCGCCCTGGCT
  GGGAATGGGCCCGTCCTGCACCTCGAGCTAGGGCAAGAAGAGGCAGAGCTGGAGGAGTTC
  CTGTGCCCTGTGAAAACACCCCCTGGGCTAGTGGGCGTGGCAGCTGCCTTGCAGCCCTTC
  CCTGCCCTTCAGAATATTTCCCTCAAGCACCTGGGGACTGAAATACAGAACACAATGCAC
  AACATCCGTGGCAACAAGCAGGCCCAGGGAACAGGCCATGAACCTCCAGGGGAAGATTCT
  ACACAGAGTGGGGAGCCTCAGAGGGAGGAGCAGCCCTCGGCCTCTGACGCCAGCACCCCA
  GTGAGAGAGCCAGAGGATTCTCCAAAGCCTGCACCCAAGCCTTCTCTGACCATCAGTTTC
  GCTCAGAAAGCCAAGCGCCAGAACAACACCTTCCCATTCTTTTCTGAAGGAATCACACGG
  AACCGAACTGCCCAGGAGAAAGTGGCAGCCTTGGAGCAACAGGTTCTGATGCTCACCAAG
  GAGTTAAAGTCTCAGAAGGAGCTAGTGAAGATCCTGCACAAGGCACTGGAGGCCGCCCAG
  CAGGAGAAGCGGGCGTCCAGCGCATACCTGGCGGCGGCTGAGGACAAGGACCGGCTGGAG
  CTGGTGCGGCACAAAGTGCGGCAGATCGCGGAGCTGGGCCGGCGGGTGGAGGCCCTGGAG
  CAGGAGCGGGAGAGCCTGGCGCACACAGCGAGCCTGCGGGAGCAGCAGGTGCAGGAGCTA
  CAGCAGCACGTGCAGCTGCTTATGGACAAGAACCACGCCAAGCAGCAGGTCATCTGCAAG
  CTCTCTGAGAAGGTCACCCAGGACTTCACGCACCCCCCTGACCAGTCTCCTTTGCGCCCC
  GACGCTGCCAACAGGGACTTCCTGAGCCAGCAGGGGAAGATAGAGCACCTGAAGGATGAC
  ATGGAAGCTTACCGGACCCAGAACTGCTTCCTCAACTCCGAGATCCACCAGGTCACAAAG
  ATCTGGAGAAAGGTGGCTGAGAAGGAGAAGGCCCTTCTGACGAAGTGCGCCTACCTCCAA
  GCCAGAAACTGCCAGGTGGAAAGCAAGTACCTGGCCGGTCTGAGAAGGCTGCAGGAGGCC
  CTGGGGGACGAAGCCAGCGAGTGCTCAGAGCTGCTGAGGCAGCTTGTCCAGGAGGCACTG
  CAGTGGGAAGCTGGGGAGGCCTCATCTGACAGCATCGAGCTGAGCCCCATCAGTAAGTAT
  GATGAGTACGGCTTCCTGACGGTGCCCGACTATGAGGTGGAAGACCTGAAGCTGCTGGCC
  AAGATCCAGGCGTTGGAGTCACGATCCCACCACCTGCTGGGCCTCGAGGCTGTGGATCGG
  CCGCTGAGGGAGCGCTGGGCTGCCCTGGGCGATCTTGTGCCCTCAGCCGAGCTCAAGCAG
  CTACTGCGGGCAGGAGTACCCCGTGAACACCGGCCTCGTGTCTGGAGGTGGCTGGTCCAC
  CTCCGTGTCCAGCACCTGCACACTCCAGGCTGCTACCAGGAACTGCTGAGCCGGGGCCAG
  GCCCGCGAGCACCCTGCTGCCCGCCAGATTGAGCTGGACCTGAACCGGACCTTCCCCAAC
  AACAAACACTTCACCTGCCCCACCTCCAGCTTCCCCGACAAGCTCCGCCGGGTGCTGCTG
  GCCTTCTCCTGGCAGAACCCCACCATCGGCTACTGCCAGGGCCTGAACAGGCTGGCGGCC
  ATTGCCCTGCTGGTCCTAGAGGAGGAGGAGAGCGCCTTCTGGTGCCTGGTGGCCATTGTG
  GAGACCATCATGCCCGCTGATTACTACTGCAACACGCTGACGGCATCCCAGGTGGACCAG
  CGGGTGCTCCAGGACCTGCTCTCGGAGAAGCTGCCCAGGCTGATGGCCCATCTGGGGCAG
  CACCACGTGGATCTCTCCCTCGTCACCTTCAACTGGTTCCTCGTGGTCTTTGCGGACAGT
  CTCATTAGCAACATCCTCCTTCGGGTCTGGGATGCCTTCCTGTACGAGGGGACGAAGTAC
  AACGAGAAGGAGATCTTGAGGCTACAGAATGGCCTGGAAATCTACCAGTACCTGCGCTTC
  TTCACCAAGACCATCTCCAACAGCCGGAAGCTGATGAACATCGCCTTCAATGACATGAAC
  CCCTTCCGCATGAAACAGCTGCGGCAGCTGCGCATGGTCCACCGGGAGCGGCTGGAGGCT
  GAGCTGCGGGAGCTGGAGCAGCTTAAGGCAGAGTACCTGGAGAGGCGGGCATCCCGGCGC
  AGAGCTGTGTCCGAGGGCTGTGCCAGCGAGGACGAGGTGGAGGGGGAAGCCTGA
  
  Clone variation with respect to AY026527.1
  721 c=>a;758 t=>c;782 g=>t;1418 t=>c;1752 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for AY026527 unedited
  GGTCCCTCCCGGTCCCTTGCCGCTTTAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATAT
  AAGCAGTAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGC
  CGCGAATTCGGCACGAGGCCGCACCCGGTCCCGACTCTGTGGCTTCCTGGGGGCGGGTTC
  GCCGTCGGCCCCGCCCCCGCCCAGGTGTCTCCCTTTTTTATCTGCCCGCCGAGTCTCCGA
  GATTTGTCCCTGGTGGTCCCGCGGACCCCTCGTCCCTCCGCAGTCTCCGGCTGGCAGCGA
  TGGAGGGCGCTGGGGAGAACGCCCCGGAGTCCAGCTCCTCTGCCCCTGGGTCCGAAGAGT
  CTGCCAGGGATCCACAGGTGCCGCCTCCGGAGGAAGAATCGGGGGACTGCGCCCGGTCCC
  TGGAGGCGGTCCCCAAGAAACTCTGTGGGTATTTAAGTAAGTTCGGCGGCAAAGGGCCCA
  TCCGGNGCTGGAAATCCCGCTGGTTCTTCTACGACGAAAGGAAATGTCAGCTGTATTACT
  CGCGGACCGCTCAGGATGCCAATCCCTTGGACAGCATCGACCTCTCCAGTGCAGTGTTTG
  ACTGTAAGGCGGACGCTGAGGAGGGGATCTTCGAAATCAGACTCCCAGCCGGGTTATTAC
  CCTGAAAGCCCCCACCCAGCAAGCGATGCTGTACTGCCTGCACCAGCTGCAGATGAAGCC
  CTGGGGATTCCCACACAGCCCGGCGGCACCTCCTGCCACCCCTTATCCCGCCCTGCCTGG
  GAATGGCCCCCTCCCTGCCCTCAAGCTAGGGCAGAATAAGCAAAACCTGGAGGAATCCTT
  TGCCCTGTGAAAACCCCCCCGGGCCTATGGGCGTTGCAACTGCCTTTAAACCCTTCCCTG
  CCCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AY026527 unedited
  CATGGTACGTGCCGCAATCNANGATCGGTTTTTTTTTTTTTTTTTNTCCCATTTACATTG
  TTTTATATTGATATAACCTAGGGAATGTTCCAGGTTACCCTATAGAGGCAGTGCTCTTGC
  TTGTTTACATGCCAAAGTGCTCTGTGGAAGAGGTGACGAAAGGGTGGCATCCCTGGGTAA
  GTAAGTGCCTATGAAGAAGTAGCCCAACCAATGGCTTTGCAGCACACAATGTCCCAGTGG
  GGAAACTGAGGGCCAGAGAGGGGAAGGGACATGTCCAAGGTCACATGGTGATGGGACACC
  CAGGGCTGGGCCACTGGTCCGTGCCTGACCTCCAGTGGGTCTGCCAGCCAAGGGTGAGGA
  AGGCTGTGGGGAGGGGAGGTGGCCAAGTCAGGCTTCCCCCTCCACCTCGTCCTCGCTGGC
  ACAGCCCTCGGACACAGCTCTGCGCCGGGATGCCCGCCTCTCCAGGTACTCTGCCTTAAG
  CTGCTCCAGCTCCCGCAGCTCAGCCTCCAGCCGCTCCCGGTGGACCATGCGCAGCTGCCG
  CAGCTGTTTCATGCGGAAGGGGTTCATGTCATTGAAGGCGATGTTCATCAGCTTCCGGCT
  GTTGGAGATGGTCTTGGGGAAGAAGCGCAGGTACTGGTAGATTTTCAGGCCATTCTGGAG
  CCTCAAGATCTCCTTCTCGTTGTACTTGAAAATGGCCAAGGCATACCGAAACAACCCCTT
  CTTCCCCTCGTACAGGAAGGCATCCCAAACCCGAAGGAGGATGTTGCTTATGAGACTGTC
  CCCAAAGACCCCGAGGAACCCCTTTGAAGTGACGAGGGAGAGATCCACCTGGTGCTCGCC
  CCAAATGGCCCTCAACCTGGGCGGGTTTTTCCGAAGGCAGTTCTGTGACACCCCCGTGGT
  CCACCTGGGGTTCCCTCAGCGTGTTGGCATATTAATACAACGGATTGATGTGTTCCACCA
  TGCGCCCCACGTCCCGCA
  
• Restriction Sites: NotI-NotI
• ACCN: AY026527
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AY026527.1, AAK07684.1
• RefSeq Size: 3257 bp
• RefSeq ORF: 2754 bp
• Locus ID: 55357
• Cytogenetics: 9q22.33
• Domains: TBC, PH
• Gene Summary: Acts as GTPase-activating protein for RAB7A. Signal effector acting as a linker between RAC1 and RAB7A, leading to RAB7A inactivation and subsequent inhibition of cadherin degradation and reduced cell-cell adhesion.[UniProtKB/Swiss-Prot Function]