LIMS2 (AF484961) Human Untagged Clone

CAT#: SC105043

(untagged)-Human PINCH-2 mRNA, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LIMS2
• Synonyms: LGMD2W; PINCH-2; PINCH2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AF484961, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACGGGAAGCAATATGTCGGACGCCTTGGCCAACGCCGTGTGCCAGCGCTGCCAGGCCCGCTTCTCCC
  CCGCCGAGCGCATTGTCAACAGCAATGGGGAGCTGTACCATGAGCACTGCTTCGTGTGTGCCCAGTGCTT
  CCGGCCCTTCCCCGAGGGGCTCTTCTATGAGTTTGAAGGCCGGAAGTACTGCGAACACGACTTCCAAATG
  CTGTTTGCTCCGTGCTGTGGATCCTGCGGTGAGTTCATCATTGGCCGCGTCATCAAGGCCATGAACAACA
  ACTGGCACCCGGGCTGCTTCCGCTGCGAGCTGTGTGATGTGGAGCTGGCTGACCTGGGCTTTGTGAAGAA
  TGCCGGCAGGCATCTCTGCCGGCCTTGCCACAACCGTGAGAAGGCCAAAGGCCTGGGCAAGTACATCTGC
  CAGCGGTGCCACCTGGTCATCGACGAGCAGCCCCTCATGTTCAGGAGCGACGCCTACCACCCTGACCACT
  TCAACTGCACCCACTGTGGGAAGGAGCTGACAGCCGAGGCCCGCGAGCTGAAGGGTGAGCTCTACTGCCT
  GCCCTGCCATGACAAGATGGGCGTCCCCATCTGCGGGGCCTGCCGCCGGCCCATCGAGGGCCGAGTGGTC
  AACGCGCTGGGCAAGCAGTGGCACGTGGAGCACTTTGTCTGTGCCAAGTGTGAGAAGCCATTCCTGGGGC
  ACCGGCACTATGAGAAGAAGGGCCTGGCCTACTGCGAGACTCACTACAACCAGCTCTTCGGGGACGTCTG
  CTACAACTGCAGCCATGTGATTGAAGGCGATGTGGTGTCGGCCCTCAACAAGGCCTGGTGTGTGAGCTGC
  TTCTCCTGCTCCACCTGCAACAGCAAGCTCACCCTGAAGGACAAGTTTGTGGAGTTCGACATGAAGCCCG
  TGTGTAAGAGGTGCTACGAGAAGTTCCCGCTGGAGCTGAAGAAGCGGCTGAAGAAGCTGTCGGAGCTGAC
  CTCCCGCAAGGCCCAGCCCAAGGCCACAGACCTCAACTCTGCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AF484961 unedited
  NNGGTTCACAATTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCACCCC
  CGCCCACCCCTCTCGCGGGCGGAGCGGCTCTTGGCCTTTTTTGGGCGTCTCCCTGCTCCG
  CGGCCCGGGCTGGCGGGCGGGCGCTCGGCTGGCGGCTGCAGCAGCAGAGGGAGACCCGCG
  GCAACCCCGGCAACCCAGGGCTCGGCGTCGCTGCCACCATGACGGGAAGCAATATGTCGG
  ACGCCTTGGCCAACGCCGTGTGCCAGCGCTGCCAGGCCCGCTTCTCCCCCGCCGAGCGCA
  TTGTCAACAGCAATGGGGAGCTGTACCATGAGCACTGCTTCGTGTGTGCCCAGTGCTTCC
  GGCCCTTCCCCGAGGGGCTCTTCTATGAGTTTGAAGGCCGGAAGTACTGCGAACACGACT
  TCCAAATGCTGTTTGCTCCGTGCTGTGGATCCTGCGGTGAGTTCATCATTGGCCGCGTCA
  TCAAGGCCATGAACAACAACTGGCACCCGGGCTGCTTCCGCTGCGAGCTGTGTGATGTGG
  AGCTGGCTGACCTGNGCTTTGTGAAGAATGCCGGCAGGCATCTCTGCCGGCCTTGCCACA
  ACCGTGAGAAGGCCAAGGGCCTGGGCAAGTACATCTGCCAGCGGTGCCACCTGGTCATCG
  ACGAGCAGCCCCTCATGTTCAGGAGCGACGCCTACCACCCTGACCACTTCAACTGCACCC
  ACTGTGGGAAGGAGCTGACAGCCGAGGGCCGCGAGCTGAAGGGTGAGCTCTACTGCCTGC
  CCTGCCATGANCAGATGGGCGTCCNCATCTGCNGNGCCCTGCCGCCCGGCCCATCGAGGC
  CCCGAGTGGNTCAACGCGCTGGGCAAGCAGTGGCACGTGGAGCACTTTGTCTGTGCCAGT
  GTGAGAAGCCANCCTGGGCACCGCACTTGAAAAGAAGGGCCTGGCTACTGCGAGATCACT
  
• Restriction Sites: NotI-NotI
• ACCN: AF484961
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AF484961.1, AAM97589.1
• RefSeq Size: 1026 bp
• RefSeq ORF: 1026 bp
• Locus ID: 55679
• Cytogenetics: 2q14.3
• Domains: LIM
• Gene Summary: This gene encodes a member of a small family of focal adhesion proteins which interacts with ILK (integrin-linked kinase), a protein which effects protein-protein interactions with the extraceullar matrix. The encoded protein has five LIM domains, each domain forming two zinc fingers, which permit interactions which regulate cell shape and migration. A pseudogene of this gene is located on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]