IFT43 (AK056735) Human Untagged Clone

CAT#: SC103104

(untagged)-Human cDNA FLJ32173 fis, clone PLACE6000953

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IFT43
• Synonyms: C14orf179; CED3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK056735, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGATTTGCTCGACTTGGACGAGGAGCTTCGCTACAGCTTGGCTACCTCCAGGGCCAAGATGGGTC
  GCCGAGCTCAACAGGAGTCAGCGCAGGCCGAGAATCACCTCAATGGCAAGAATTCCTCTTTGACTCTGAC
  TGGAGAGACTTCCTCTGCTAAATTACCTCGCTGCCGACAGGGAGGCTGGGCAGGTGATTCCGTGAAGGCT
  TCGAAGTTTAGGAGGAAGGCTTCTGAAGAAATAGAAGATTTCCGCCTCAGACCACAGAGCCTGAATGGAT
  CAGATTATGGAGGAGCGGTACCCAAACAGGCAAACAACAGCTGGATCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK056735 unedited
  GGGGTTCACATTTGTATACGACTCACTTAGGCGGCCGCGAAATTCGCACGAGGGGAGAAT
  GGAGATTTGCTCGACTTGGACGAGGAGCTTCGCTACAGCTTGGCTACCTCCAGGGCCAAG
  ATGGGTCGCCGAGCTCAACAGGAGTCAGCGCAGGCCGAGAATCACCTCAATGGCAAGAAT
  TCCTCTTTGACTCTGACTGGAGAGACTTCCTCTGCTAAATTACCTCGCTGCCGACAGGGA
  GGCTGGGCAGGTGATTCCGTGAAGGCTTCGAAGTTTAGGAGGAAGGCTTCTGAAGAAATA
  GAAGATTTCCGCCTCAGACCACAGAGCCTGAATGGATCAGATTATGGAGGAGATATTCCT
  ATCATTCCGGATCTGGAGGAAGTACAGGAAGAAGACTTTGTTTTGCAGGTGGCAGCCCCT
  CCCAGCATCCAGATAAAGCGGGTGATGACCTACCGTGACCTGGACAATGACCTCATGAAG
  TACTCAGCCATTCAGACACTGGATGGGGAGATCGACCTGAAACTCCTCACCAAAGTGCTC
  GCGCCGGAGCACGAAGTCCGGGAGGATGATGTCGGCTGGGACTGGGACCATCTGTTCACT
  GAGGTGTCCTCAGAGGTCCTCACTGAGTGGGACCCACTGCAGACGGAGAATGANGACCCT
  GCGGGGCATGCCAGGCACACCTGAGCCCGTCACCCATGCTCTAGACATGAAGAAAATGCA
  TGAGCTTAAAGCTAAAGAAGCTTGTAAGCAGCTCCGAATTTTTACCTGNAATATTTTGTA
  ATAAAAATATTTATATTCAGTCAACCACATTGGATAATTCAATTGCAATAAATTGCTTAT
  TCTGTGCCATCTNCAAAAAAAAAAAANAAAACTCGACTCTAGATTGCGGCCGCGGGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK056735 unedited
  ACCGCGGCCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGGGAGAGGCACAGAAT
  AAGCAATTTATTGCAATTGAATTATCCAATGTGGTTGACTGAATATAAATATTTTTATTA
  CAAAATATTCCAGGTAAAAATTCGGAGCTGCTTACAAGCTTCTTTAGCTTTAAGCTCATT
  GCATTTCTTCATGTCTAGAGCATGGGTGACGGGCTCAGGTGTGCCTGGCCTGCCCCGCAG
  GGTCCTCCTTCTCCGTCTGCAGTGGGTCCCACTCAGTGAGGACCTCTGAGGACACCTCAG
  TGAACAGATGGTCCCAGTCCCAGCCGACATCATCCTCCCGGACTTCGTGCTCCGGCGCGA
  GCACTTTGGTGAGGAGTTTCAGGTCGATCTCCCCATCCAGTGTCTGAATGGCTGAGTACT
  TCATGAGGTCATTGTCCAGGTCACGGTAGGTCATCACCCGCTTTATCTGGATGCTGGGAG
  GGGCTGCCACCTGCAAAACAAAGTCTTCTTCCTGTACTTCCTCCAGATCCGGAATGATAG
  GAATATCTCCTCCATAATCTGATCCATTCANGCTCTGTGGTCTGAGGCGGAAATCTTCTA
  TTTCTTCAGAAGCCTTCCTCCTAAACTTCGAAGCCTTCACGGGATCACCTGCCCAGCCTC
  CCTGTCNGCAGCGAGGTAATTTAGCAGAAGAAGTCTCTCCAGTCAGAGTCAAAGAGGAAT
  TCCTGCCATTGGAGTGATTCTCGGCCTGCGCTGACTCCTGGTGAGCTCNGCGACCCATCT
  GGCCCTGGAGTAGCCAAGCTGTAGCGAAGCTCCTCCTCCCAGTCGAGCAAATTCTCCATC
  TTCCCTCGTGCCGAAATTGCGGGCGCCCTATAGTGGAGTCTATTACAAAAATCTGACGGG
  TCACTAAACGAGCTCTGGCTAATTAGACCTCCCACCGGACACGCCTACGCGCCATTTGGG
  TAAACGGGCGGGGGTATAACCACACTTTGGAAAATCCCGTGGATTTGGGGCCCAAACAAA
  CTTCCTTGCGGCATGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: AK056735
• Insert Size: 900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK056735.1, BAG51798.1
• RefSeq Size: 1096 bp
• RefSeq ORF: 330 bp
• Locus ID: 112752
• Cytogenetics: 14q24.3
• Gene Summary: This gene encodes a subunit of the intraflagellar transport complex A (IFT-A). IFT-A is a multiprotein complex that plays an important role in cilia assembly and maintenance by mediating retrograde ciliary transport. Mutations in this gene are a cause of cranioectodermal dysplasia-3 (CED3), also known as Sensenbrenner syndrome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]