MRPL4 (NM_146388) Human Untagged Clone

CAT#: SC101186

MRPL4 (untagged)-Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRPL4
• Synonyms: CGI-28; L4mt
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_146388, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGCAGTTCGTCCGGGCCGGGGCGCGGGCCTGGCTTCGGCCTACCGGCAGCCAGGGCCTGAGTTCCC
  TGGCGGAAGAGGCAGCGCGTGCGACCGAGAACCCGGAGCAGGTGGCGAGCGAGGGTCTCCCGGAGCCCGT
  GCTGCGCAAAGTCGAGCTCCCGGTACCCACTCATCGACGCCCAGTGCAGGCCTGGGTCGAGTCCTTGCGG
  GGCTTCGAGCAGGAGCGCGTGGGCCTGGCCGACCTGCACCCCGATGTTTTCGCCACCGCGCCCAGGCTGG
  ACATACTGCACCAGGTTGCTATGTGGCAGAAGAACTTCAAGAGAATTAGCTATGCCAAGACCAAGACGAG
  AGCCGAGGTGCGGGGCGGTGGCCGGAAGCCTTGGCCGCAGAAAGGCACTGGGCGGGCCCGGCATGGCAGC
  ATCCGCTCTCCGCTCTGGCGAGGAGGAGGTGTTGCCCATGGCCCCCGGGGCCCCACAAGTTACTACTACA
  TGCTGCCCATGAAGGTGCGGGCGCTGGGTCTCAAAGTGGCACTGACCGTCAAGCTGGCCCAGGACGACCT
  GCACATCATGGACTCCCTAGAGCTGCCCACCGGAGACCCACAGTACCTGACAGAGCTGGCGCACTACCGC
  CGCTGGGGGGACTCCGTACTCCTCGTGGACTTAACACACGAGGAGATGCCACAGAGCATCGTGGAGGCCA
  CCTCTAGGCTTAAGACCTTCAACTTGATCCCGGCTGTTGGTGAGCAAAGAGCCCAGGCCCCTAGAGTGCG
  CATGTGCAGGCTCCGCTGTTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_146388 unedited
  NNCCTGTTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACCAGCCAAAG
  TAGCTAGGCCTACGGAAGCTGGGTCTTCTTGCTGTGAGGTCGCGTTCCCCAGTGTTACGG
  AGGGTCCTTGAGGCAGGAGTGAAAATTGGGTCTGGGGGTTAGTCCTGGGGTGGAGGTCTG
  GGCACGCCGGGTCGGACCCCCTCCATCTTCGGTTTTGCACACCCCGCTTTCCAGCGCGGA
  GTCGCGGCGGGTAGGGCGGCGTCGCGTGCGTGACGTCATCCAGCGGCGCCTCGCGAGGCT
  CCAGTGGCCTTGACCTCCCGCGGCGTGGGAGGCTGCGCGGCGATGCTGCAGTTCGTCCGG
  GCCGGGGCGCGGGCCTGGCTTCGGCCTACCGGCAGCCAGGGCCTGAGTTCCCTGGCGGAA
  GAGGCAGCGCGTGCGACCGAGAACCCGGAGCAGGTGGCGAGCGAGGGTCTCCCGGAGCCC
  GTGCTGCGCAAAGTCGAGCTCCCGGTACCCACTCATCGACGCCCAGTGCAGGCCTGGGTC
  GAGTCCTTGCGGGGCTTCGAGCAGGAGCGCGTGGGCCTGGCCGACCTGCACCCCGATGTT
  TTCGCCACCGCGCCCAGGCTGGACATACTGCACCAGGTTGCTATGTGGCAGAAGAACTTC
  AAGAGAATTAGCTATGCCAAGACCAAGACGAGAGCCGAGGTGCGGNNGCGGTGGCCGAAA
  GCCTTGGCCGCAGAAAGCACTGGGCGGGCCCGGCATGGCAGCATCCGCTCTTCGCTCTGG
  CGAGGANGNANGTGTTGCCCATGGGGCCCCCGGGCCCCACAAGTTACTACTACATGCTGC
  CNNATGAAGTGCGGNCGCTGGNTCTCAAAGTGCACTGACCGTCAAGCTGGCCAGGACGAC
  TGCCATCATGGACTCCCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_146388
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146388.1, NP_666500.1
• RefSeq Size: 2404 bp
• RefSeq ORF: 792 bp
• Locus ID: 51073
• UniProt ID: Q9BYD3
• Cytogenetics: 19p13.2
• Domains: Ribosomal_L4
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, compared to variant 1, that results in a frameshift. It encodes an isoform (b) which has a shorter and distinct C-terminus compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.