GAPT (NM_152687) Human Untagged Clone

CAT#: SC100631

GAPT (untagged)-Human GRB2-binding adaptor protein, transmembrane (GAPT)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GAPT
• Synonyms: C5orf29
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100631 sequence for NM_152687 edited (data generated by NextGen Sequencing)
  ATGTCGAAAAGCTGTGGAAATAATTTAGCGGCCATTTCTGTAGGAATTTCGCTTCTTTTA
  CTCTTAGTGGTTTGTGGAATTGGGTGTGTTTGGCACTGGAAACACCGTGTTGCCACACGA
  TTTACCTTACCGAGGTTTTTACAAAGGAGAAGCAGCAGGAGAAAAGTCTGTACTAAAACA
  TTCTTGGGCCCCCGCATCATTGGCTTAAGGCATGAAATCTCAGTTGAAACCCAAGACCAC
  AAATCTGCTGTCAGGGGAAATAACACACACGACAACTATGAAAATGTGGAAGCAGGTCCT
  CCCAAAGCTAAAGGAAAAACCGATAAGGAACTATATGAAAACACAGGGCAGTCTAATTTC
  GAGGAGCATATCTATGGAAATGAGACATCTTCTGACTATTATAACTTCCAGAAGCCTCGT
  CCTTCTGAAGTTCCTCAAGATGAAGATATATACATTCTTCCAGATTCATATTAG
  
  Clone variation with respect to NM_152687.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152687 unedited
  GTCACCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTTGACACAC
  CAATCTTGAGGTGTTGTGCCATTCTCTGGGAACATACAGATGAAATGACCTGAACCCTGC
  CCACCATTTAACCAACAGGTTTCTGGATGGCAGATCATTCAACCTTCTTTTTCTTGAGTT
  TTCTACTCTGCTGTCATCTTCCACTCATTTCTGCTTCATTTTGTGATTCGTTAACAGAAA
  AGGAAGTGGAATGGAATAAAATCCCCCAATACAGTACAATTATACATTAATGGCTGTAAT
  GTGAAGAGCATCACACACGAAGAGAGCCATCTTCCAGAAATAAGTTTATACACTCTCTCC
  TCTAATTGCATCAGGACTTTACCAGATAATGTTCTTCCAGATCTGAAAAGGAAAATGCCT
  AAAAGAGCTTCCAAACTCATTTTTGAATAATACTAGGCTACAAAGAATTACACTGTGAAT
  TCATTAAGGGTAACACCAAATCACTAAACAGCACTGTTTGTACAGAAATGTCGAAAAGCT
  GTGGAAATAATTTAGCGGCCATTTCTGTAGGAATTTCGCTTCTTTTACTCTTAGTGGTTT
  GTGGAATTGGGTGTGTTTGGCACTGGAAACACCGTGTTGCCACACGATTTACCTTACCGA
  GGTTTTTACAAAGGAGAAGCAGCAGGAGAAAAGTCTGTACTAAAACATTCTTGGGCCCCC
  GCATCATTGGCTTAAGGCATGAAATCTTCAGTGAAACCCAAGACCCCAATCTGCTGTCAG
  GGGAAATAACACCCACGACAACTATGGAAATGTGGAAGCAGGTCCTTCCCAAGCTAAAGG
  AAAAACCGTTAGGGACTCTTTGAAAACACAGGNCAGTCTAATTTCGAGAGCATTTTATGG
  GAATGAGACACTNCTGACTTTATAACTTCCAAAACCTCGTTCTTCTGGAGTTNCTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_152687 unedited
  GATCGGTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTAGGGGGGAATAACAAATGAATTTATTAAATTTTTTTCTCAAAACTACCCAGCAAAATG
  TACAATTCAATTCCCGTCAGTAAAACATGAGGCATTTAGGGCCTTATGGGATGTTCTAGG
  CATTTGGTCTTTTTCTTTTCATTTAGGGGGGAATTTAAAACATTTTCATATGAAGCTAGG
  GAAAAAAATGCTGGGGCAACATTCATATGTTTAAATGTCCCAAAAATTGAAAAGGCCTCA
  AAGCTTATTTCAAAAAATAAAAAGGGAAATGCCGGTTTGGAGTGATTCAGGAAATGTGGG
  GAAAAACTTATTATCCGAACCCCGCTTGAGAAAAAAGGCCAAAAAACAAAGCTTTGAAGG
  AAGGAAAAATTTGGCAGGGTTTAAAAAGATTCTGCCGAAGGCCAAAAAATGGGAGATAGT
  TAAAAAAGTCTCTGGAAAAATGAATTGGACCCCCTTGTGGAAAACCCTAAATTTTGGAGT
  TCAAAATATGGGGATTATGTCTTTTTCAAAGAGGGGGGCTTAAAACCTTTTAAGTGAAAA
  AAAAAAGGTGGGCGGGTAGAAAATTTTTTGAAAAAAAAACTGGCGCCACCCCCCGGGAAA
  TGGATGGGGGGGGGGAGAAAACCTAACCCCGGGGGGAACAGTTTTTTGGCCCCTTGACAA
  TATTTTTTGGGCCCAAAAAATAAAATGGGGAAAAAAAAAGGAAAGAAATACGCGGGTATT
  TTTAGAGGGGAAAAACCTTTCCCCCAATCCCGAATTTATCTGGGGGCGGGGGGGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_152687
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152687.1, NP_689900.1
• RefSeq Size: 2218 bp
• RefSeq ORF: 474 bp
• Locus ID: 202309
• UniProt ID: Q8N292
• Cytogenetics: 5q11.2
• Protein Families: Transmembrane
• Gene Summary: Negatively regulates B-cell proliferation following stimulation through the B-cell receptor. May play an important role in maintenance of marginal zone (MZ) B-cells (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) has three alternate splice sites in the 5' UTR and encodes the same protein, compared to variant 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.