KCTD6 (NM_153331) Human Untagged Clone

CAT#: SC100338

KCTD6 (untagged)-Human potassium channel tetramerisation domain containing 6 (KCTD6), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCTD6
• Synonyms: KCASH3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100338 sequence for NM_153331 edited (data generated by NextGen Sequencing)
  ATGGATAATGGAGACTGGGGCTATATGATGACTGACCCAGTCACATTAAATGTAGGTGGA
  CACTTGTATACAACGTCTCTCACCACATTGACGCGTTACCCGGATTCCATGCTTGGAGCT
  ATGTTTGGGGGGGACTTCCCCACAGCTCGAGACCCTCAAGGCAATTACTTTATTGATCGA
  GATGGACCTCTTTTCCGATATGTCCTCAACTTCTTAAGAACTTCAGAATTGACCTTACCG
  TTGGATTTTAAGGAATTTGATCTGCTTCGGAAAGAAGCAGATTTTTACCAGATTGAGCCC
  TTGATTCAGTGTCTCAATGATCCTAAGCCTTTGTATCCCATGGATACTTTTGAAGAAGTT
  GTGGAGCTGTCTAGTACTCGGAAGCTTTCTAAGTACTCCAACCCAGTGGCTGTCATCATA
  ACGCAACTAACCATCACCACTAAGGTCCATTCCTTACTAGAAGGCATCTCAAATTATTTT
  ACCAAGTGGAATAAGCACATGATGGACACCAGAGACTGCCAGGTTTCCTTTACTTTTGGA
  CCCTGTGATTATCACCAGGAAGTTTCTCTTAGGGTCCACCTGATGGAATACATTACAAAA
  CAAGGTTTCACGATCCGCAACACCCGGGTGCATCACATGAGTGAGCGGGCCAATGAAAAC
  ACAGTGGAGCACAACTGGACTTTCTGTAGGCTAGCCCGGAAGACAGACGACTGA
  
  Clone variation with respect to NM_153331.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_153331 unedited
  TTTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGCTCCGGCCGCGGGC
  ATCTCCCGGCCCGGCCGCAGCAGCCGCCGCCGCCGCGCATTTCCCTGAAACCTGGGCTCT
  TGAAGACGCATCACTGGAGCAGATGGATAATGGAGACTGGGGCTATATGATGACTGACCC
  AGTCACATTAAATGTAGGTGGACACTTGTATACAACGTCTCTCACCACATTGACGCGTTA
  CCCGGATTCCATGCTTGGAGCTATGTTTGGGGGGGACTTCCCCACAGCTCGAGACCCTCA
  AGGCAATTACTTTATTGATCGAGATGGACCTCTTTTCCGATATGTCCTCAACTTCTTAAG
  AACTTCAGAATTGACCTTACCGTTGGATTTTAAGGAATTTGATCTGCTTCGGAAAGAAGC
  AGATTTTTACCAGATTGAGCCCTTGATTCAGTGTCTCAATGATCCTAAGCCTTTGTATCC
  CATGGATACTTTTGAAGAAGTTGTGGAGCTGTCTAGTACTCGGAAGCTTTCTAAGTACTC
  CAACCCAGTGGCTGTCATCATAACGCAACTAACCATCACCACTAAGGTCCATTCCTTACT
  AGAAGGCATCTCANATTATTTTACCAAGTGGAATAAGCACATGATGGACACCAGAGACTG
  CCNAGTTTCCTTTACTTTTGGACCCTGTGATTATCACCAGGAAGTTTCNTCTTAGGGTCC
  ACCTGATGGAATACATTACAAAACAAGGNTTCACGATCCGCAACACCCGGGTGCATCACA
  TGAGTGAGCGGGCCAATGAAACACAGTGGAGCACAACTGGACTTTTCTGTAGCTAGCCCG
  NAAGACGACGACTGATCTNCGACCCTGCCACAGTTCCTGGAAAGACTCTCAGAATGGAAG
  AACTGATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_153331 unedited
  NNAAATTACTTGNACCGCGCCGCATTCTANNGATCGGTTTTTTTTTTTTTTTTTTAATTT
  TCATATTTCATTTTTTATTAGTTTTGTTTTTTTTTTCTGGTAGACAGTACACTTGGGAAT
  TATACTGCACCAGGCATAAAGAGTAAGTTTTCTACCACAGGGACATTTTGTATTCAGAAT
  TCAATATAAATATTTCTAGTCAGACATTTCCATGGCTACAGATATTTGGTTCCTTGATTT
  ATATGCATAGAAAGAAACAGTTGTCATAACTGTAAAAAGCAGTACTTAATAAGTACTTTT
  AAATGATTGGAACAGTTTTCCTTTAATATTACAATACTACTTATTTATTGGTTTGGAAAC
  TAGGTCACCCTACATGATGTTTTGATTTTTCTCAAAGGCATCATGAGTACTCTGCTTATT
  CTTTCATTCTTGTATTTTTAGCCTTCTAGTTGAGTTAGGGACCATTTTATCAGAAATCAT
  TTTAGCACTGTAATAAGAAAAGCTCTGTTAAGGGTAGATTATATACCATAATGATTTTCA
  GACTTCTTTTTATAAAAAAGGTATCTGGAGACTCTGAACAGGCACATGCATACTCAGGGG
  GAAGGGAACAAAACATGGAGGAGTCTGCCAAAGCACAAAACTTCCTTCTGGTCCTCACTG
  CCTTCAAATAAATACAACTATTTAAAAGAAAAAAAATATCTCACACTGTGATTTAAAAAA
  AAAAATCAGTATCTTCCATTTCCTGGAGAGTCTTTCCAGGAACCTGTGGCAGGGTCGGAG
  ATCAGTCGTCTGTCTTCCGGGCTAGCCTACAGAAAGTCCAGTTGTGCTCCACTGTGTTTT
  CATTGGCCCGCTCACTCATGTGATGCACCCGGGTGTTGCGGATCGTGAAACCTTGTTTTT
  GTATGTATTCCATCAGGTGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_153331
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153331.2, NP_699162.2
• RefSeq Size: 1823 bp
• RefSeq ORF: 714 bp
• Locus ID: 200845
• UniProt ID: Q8NC69
• Cytogenetics: 3p14.3
• Domains: BTB, K_tetra
• Protein Families: Ion Channels: Other
• Gene Summary: Probable substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex mediating the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes the ubiquitination of HDAC1; the function seems to depend on KCTD11:KCTD6 oligomerization. Can function as antagonist of the Hedgehog pathway by affecting the nuclear transfer of transcription factor GLI1; the function probably occurs via HDAC1 down-regulation, keeping GLI1 acetylated and inactive. Inhibits cell growth and tumorigenicity of medulloblastoma (MDB) (PubMed:21472142). Involved in regulating protein levels of ANK1 isoform Mu17 probably implicating CUL3-dependent proteasomal degradation (PubMed:22573887).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.