Chrd (NM_057134) Rat Tagged ORF Clone

CAT#: RR217355

Chrd (myc-DDK-tagged) - Rat chordin (Chrd)

Product Images

Circular map for RR217355

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chrd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217355 representing NM_057134
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAGCCTCCCGGCCCCGCCGGCCCCTCGGCTGCTCCTCGGGCTGCTGCTGCTCGGCTCGCGGCCGG
  CCGGTGGCACTGGCCCTGAGGCCCCCGCACTGCCCATCCGTTCCGAGAAGGAGCCACTGCCTGTCCGGGG
  AGCGGCAGGCTGCTCCTTCGGCGGGAAGGTCTATGCCTTGGACGAGACGTGGCACCCGGACCTGGGGGAG
  CCTTTTGGGGTGATGCGCTGTGTGCTGTGCGCCTGCGAAGCGCCTCAGTGGGCTCGCCGTGGGAGGGGCC
  CTGGCAGGGTCAGCTGCAAGAACATCAAACCTCAGTGCCCCACCCTGGCCTGCAGGCAGCCGCGCCAGCT
  GCCAGGACACTGCTGCCAGACCTGCCCGCAGGAGCGCAGCAATCTAGATCCACAGCCGGCTGGCCTGGTC
  TTCGAGTATCCAAGGGACCCAGAGCATCGCAGTTATAGCGATCGAGGGGAACCCGGCACTGGGGAGCGGA
  CACGTGGTGATGGCCACACGGACTTTGTGGCGTTGCTGACTGGACCGAGGACGCAGGCGGTAGCTCGTGC
  TCGAGTCTCTTTGCTGCGCTCAAGTTTACGCTTCTCTATCTCCTACCAGCGGCTGGACCGTCCCAGCAGG
  GTTCGGTTCACAGACCCCACAGGCAACATCCTGTTTGAACACCCTGCTGCCCCCACCCAAGATGGCCTGG
  TCTGTGGGGTGTGGCGGGCAGTGCCTCGGCTGTCTGTGAGGCTCCTGAGGGCAGAGCAGCTGCGTGTTGC
  CCTTGTGACACCCACTCACCCTTCAGAAGAAGTCTGGGGGCCTCTCATTTGGCAGGGTGCTCTCACTGCA
  GAGACCTTCAGTGCCATCCTGACCCTGGAGGACCCACTGCAGCGGGGTGTCGGGGGCATAGCCCTGCTCA
  CTCTCAGTGACACAGAAGATGCCTTACATTTTTTGCTGCTCTTCCGGGGTCTGCTGGGAGGACTAGCTCA
  CGTCCCCTTGAAGCTTCAGATTCTCCACCAGGGACAGCTACTTCGAGAGCTCCAGGCCAATGCCTCAGCT
  CAGGAGCCAGGTTTTGCTGAGGTGCTGCCCAGCCTTACAGACCAAGAGATGGACTGGTTGGTGCTGGGGG
  AGCTGCAGATGGTCCTAGAGAAGATGGGTGGGCCAGAGCTACGCATCAGCGGGTATATCACGACCAGGCA
  GAGCTGTGATGTCCTTCAAAGCGTCCTCTGTGGGGCTGATGCCTTGATCCCAGTCCAGACGGGTGCTGCT
  GGCTCAGCCAGCTTCATACTGCTAGGAAATGGCTCCCTTATCTATCAGGTACAAGTGATAGGTACAGGTA
  GCGAGGTGGTGGCCATGACGCTGGAGACCAAGCCTCAGCGGAAGAACCAGCGCACTGTCCTGTGCCACAT
  GGCTGGACTCCAGCTGGGAGGACACATGGCTGTGGGTGTCTGCTCTGGGCTGGGTGCCCGAGGGGCTCAC
  ATGCTGCTACAGAATGAGTTGTTCCTGAATATTGGTACCAAGGACTTCCCAGACGGAGAGCTTCGGGGGC
  ACGTGACTGCCCTATGCTACAGCGGGCACAGTGCCCACTACGACAGATTGCCTGTGCCTCTGGCAGGGGC
  ACTAGTACTGCCCCCTGTGCGGAGTCAGGCAGCAGGACATGCCTGGCTGTCCTTGGATACACATTGCCAC
  TTACACTATGAGGTTCTATTGGCTGGGCTTGGTGGCTCAGAGCAAGGAACTGTCACTGCTCATCTCCTCG
  GGCCTCCTGGGATGCCAGGGCCCCAGCGACTGCTGAAGGGATTCTATGGCTCAGAGGCTCAGGGTGTGGT
  AAAAGATCTAGAGCCCGTGCTGCTGCGGCACCTGACACAGGGCACCGCCTCCCTGCTTATCACCACCAAG
  AGTAACCCTAGAGGAGAGCTACGCGGGCAGGTGCACATTGCCAGTCAGTGTGAGGTGGGAGGCCTGCGCC
  TGGCCTCAGAAGGAGTGCGGATGTCCTTGGCTCCAAATGGAGAGGCAGCTACATCACCCATGCTGCCTGC
  TGGGCCCGGCCCTGAAGCCCCCGTACCAGCCAAACACGGCAGCTCTGGGAGACCCCGAGATCCTAACACA
  TGTTTCTTCGAGGGGCAGCAGCGTCCCCACGGGGCTCGCTGGGCACCCAACTATGACCCACTCTGCTCCC
  TCTGCACCTGCCAGAGACGGACAGTGATCTGTGATCCTGTAGTATGCCCACCACCAAGCTGTCCCCACCC
  GGTGCAGGCACTGGACCAGTGCTGTCCCGTGTGTCCAGAGAAACAACGCAGTAGAGACCTTCCCAGCCTA
  CCAAATCTGGAGCCAGGAGAGGGCTGCTATTTTGATGGTGACCGGAGCTGGAGGGCAGCGGGTACCCGAT
  GGCACCCCGTTGTGCCCCCCTTTGGCCTAATTAAGTGTGCTGTCTGCACCTGCAAGGGGGCCACCGGAGA
  GGTGCACTGTGAGAAGGTGCAGTGTCCTCGCCTCGCCTGTGCTCAGCCTGTCCGTGCCAACCCCACCGAC
  TGCTGCAAACAGTGCCCAGTAGGGTCAGGGACTCATGCCAAGCTGGGAGACCCCATGCAGGCTGATGGGC
  CTCGGGGTTGTCGCTTTGCTGGGCAGTGGTTCCCAGAGAATCAGAGCTGGCACCCATCAGTGCCCCCCTT
  TGGGGAGATGAGCTGTATTACCTGCAGATGTGGGGCAGGGGTACCCCACTGTGAGCGGGACGATTGCTCG
  CCGCCACTATCCTGCGGCTCAGGGAAGGAGAGTCGATGCTGTTCCCACTGTACAGCCCAAAGGTCCTCTG
  AGACTAGAACCCTTCCCGAGCTGGAGAAAGAAGCTCAACGCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217355 representing NM_057134
  Red=Cloning site Green=Tags(s)
  
  MPSLPAPPAPRLLLGLLLLGSRPAGGTGPEAPALPIRSEKEPLPVRGAAGCSFGGKVYALDETWHPDLGE
  PFGVMRCVLCACEAPQWARRGRGPGRVSCKNIKPQCPTLACRQPRQLPGHCCQTCPQERSNLDPQPAGLV
  FEYPRDPEHRSYSDRGEPGTGERTRGDGHTDFVALLTGPRTQAVARARVSLLRSSLRFSISYQRLDRPSR
  VRFTDPTGNILFEHPAAPTQDGLVCGVWRAVPRLSVRLLRAEQLRVALVTPTHPSEEVWGPLIWQGALTA
  ETFSAILTLEDPLQRGVGGIALLTLSDTEDALHFLLLFRGLLGGLAHVPLKLQILHQGQLLRELQANASA
  QEPGFAEVLPSLTDQEMDWLVLGELQMVLEKMGGPELRISGYITTRQSCDVLQSVLCGADALIPVQTGAA
  GSASFILLGNGSLIYQVQVIGTGSEVVAMTLETKPQRKNQRTVLCHMAGLQLGGHMAVGVCSGLGARGAH
  MLLQNELFLNIGTKDFPDGELRGHVTALCYSGHSAHYDRLPVPLAGALVLPPVRSQAAGHAWLSLDTHCH
  LHYEVLLAGLGGSEQGTVTAHLLGPPGMPGPQRLLKGFYGSEAQGVVKDLEPVLLRHLTQGTASLLITTK
  SNPRGELRGQVHIASQCEVGGLRLASEGVRMSLAPNGEAATSPMLPAGPGPEAPVPAKHGSSGRPRDPNT
  CFFEGQQRPHGARWAPNYDPLCSLCTCQRRTVICDPVVCPPPSCPHPVQALDQCCPVCPEKQRSRDLPSL
  PNLEPGEGCYFDGDRSWRAAGTRWHPVVPPFGLIKCAVCTCKGATGEVHCEKVQCPRLACAQPVRANPTD
  CCKQCPVGSGTHAKLGDPMQADGPRGCRFAGQWFPENQSWHPSVPPFGEMSCITCRCGAGVPHCERDDCS
  PPLSCGSGKESRCCSHCTAQRSSETRTLPELEKEAQRS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_057134
• ORF Size: 2844 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_057134.1, NP_476475.1
• RefSeq Size: 3267 bp
• RefSeq ORF: 2847 bp
• Locus ID: 117275
• Cytogenetics: 11q23
• MW: 101.7 kDa
• Gene Summary: secreted protein; human homolog binds TGF-beta and similar morphogenetic proteins; may be important for organogenesis and dorsal/ventral pattern specification of neural crest [RGD, Feb 2006]