Rb1 (NM_017045) Rat Tagged ORF Clone

CAT#: RR217325

Rb1 (myc-DDK-tagged) - Rat retinoblastoma 1 (Rb1)

Product Images

Circular map for RR217325

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rb1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217325 representing NM_017045
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCCCAAAGCCCCGCGCAGAACCGCGGCCGCCGAGCCCCCGCCGCCGCCGCCGCCGCCTCCGGAGG
  ACGACCCCGCGCAGGACAGTGACCCCGAAGAGCTGCCCCTGATCAGGCTTGAGTTTGAAAAAATTGAAGA
  ACCTGAATTTATTGCATTATGTCAGAAGTTAAAGGTGCCCGATCATGTCAGAGAGAGAGCTTGGCTAACG
  TGGGAGAAAGTTTCATCTGTGGATGGAATCCTGGAAGGATATATTCAGAAGAAGAAGGAACTCTGGGGCA
  TCTGCATCTTCATTGCAGCAGTTGACCTGGATGAGATGCCATTCACTTTTACTGAGCTTCAGAAAAGCAT
  AGAAACCAGTGTCTATAAATTCTTTGACTTATTAAAAGAAATTGATACCAGTACCAAAGTTGATAATGCT
  GTGTCAAGGCTATTGAAGAAGTACAATGTGTTATGTGCACTCTACAGCAAATTAGAACGGACATGTGGAC
  TTATATATTTGACACAGCCCAGCAGTGGGTTATCTACTGAAATAAATTCTATGTTGGTGCTAAAAGTTTC
  TTGGATCACCTTTTTACTAGCTAAAGGAGAAGTAGTACAGATGGAAGATGATCTGGTGATCTCATTTCAG
  TTAATGTTGTGTGTACTTGACTATTTTATCAAGCTCTCGCCTCCTGCGCTGCTCAGAGAGCCGTACAAAA
  CAGCTGCAACACCCATTAATGGTTCACCTCGGACACCCAGAAGAGGTCAGAACAGGAGTGCTCGGATAGC
  AAAACAACTAGAAAGTGATACAAGAACTATTGAAGTTCTCTGTAAGGAGCACGAGTGTAATGTAGATGAG
  GTGAAAAATGTTTATTTCAAAAATTTTATCCCTTTTATAAGTTCACTTGGAATTGTATCATCTAATGGAC
  TTCCAGAGCTTGAAAGTCTTTCTAAACGCTACGAAGAAGTTTATCTTAAAAGCAAAGATTTAGATGCAAG
  ACTATTTTTGGATCATGACAAAACACTTCAGACTGATACTATAGACAGTTTTGAAACAGAGAGAACTCCA
  CGAAAAAGCAACCCTGATGAAGAGGCAAACATGGTTACTCCACACACTCCAGTTAGGACTGTTATGAATA
  CTATTCAACAGTTAATGGTGATTTTAAATTCTGCAAGTGATCAGCCATCAGAAAATCTGATTTCCTACTT
  CAGTAATTGCACAGTGAATCCAAAAGAAAATATCCTAAAAAGAGTAAAGGATGTTGGACACATCTTTAAA
  GAGAAGTTTGCTAGCGCTGTGGGGCAGGGATGTATTGACATTGGAGCACAGCGATATAAACTTGGGGTTC
  GATTGTATTATCGTGTGATGGAATCGATGCTTAAATCAGAAGAAGAACGTTTGTCCATTCAGAATTTTAG
  CAAACTCCTAAATGACAACATCTTTCATATGTCTTTATTGGCCTGTGCTCTTGAAGTTGTAATGGCTACA
  TATAGCAGGAGTATGTTACAGAATCTTGATTCTGGAACAGATTTGTCCTTCCCGTGGATTCTGAATGTAC
  TTAATTTAAAAGCCTTTGATTTTTACAAAGTGATTGAAAGTTTTATCAAAGTGGAAGCCAACTTGACAAG
  AGAAATGATAAAACATTTAGAAAGATGTGAGCATCGAATCATGGAATCCCTTGCATGGCTTTCTGATTCA
  CCTTTGTTTGATCTCATAAAGCAGTCCAAGGATGGGGAAGGACCTGATCACCTTGAGTCTGCTTGTTCTC
  TCAGCCTCCCTCTCCAGAGTAACCACACTGCAGCAGATATGTATCTTTCTCCTATAAGATCTCCAAAGAA
  AAGAACTTCTACTACACGTGTAAATTCTGCGGCAAATACAGAGACGCAAGCAGCCTCAGCCTTCCATACT
  CAGAAGCCATTGAAATCTACCTCCCTTTCCCTGTTTTACAAAAAAGTGTACCGTCTAGCATATCTCCGAC
  TAAATACACTGTGTGCGCGCCTTCTGTCTGACCACCCAGAGCCAGAACACATCATCTGGACTCTGTTTCA
  GCATACATTGGAAAATGAGTATGAGCTCATGAAAGACCGACATTTGGACCAGATTATGATGTGTTCTATG
  TATGGCATCTGCAAGGTGAAAAACATTGACCTTAAATTCAAAATCATCGTAACTGCGTATAAGGATCTTC
  CTCACGCTGCCCAGGAGACCTTTAAACGTGTTTTGATCAGAGAAGAGGAGTTTGATTCCATTATAGTGTT
  CTACAACTCAGTTTTCATGCAGAGACTAAAAACAAATATTTTACAGTATGCCTCCACCAGGCCTCCTACC
  TTGTCACCAATACCTCACATTCCTCGAAGCCCTTACAAGTTCTCGAGTTCACCCTTGCGGATTCCTGGAG
  GTAACATCTATATGTCACCCCTAAAGAGTCCTTATAAAATTTCAGAAGGTCTGCCAACACCCACAAAAAT
  GACTCCAAGATCAAGAATCTTGGTCTCAATCGGTGAATCATTTGGGACATCCGAGAAGTTCCAGAAAATA
  AACCAGATGGTGTGTAACAGTGACAGAGTGCTCAAAAGAAGTGCTGAAGGCGGCAATCCCCCCAAACCCC
  TGAAGAAGCTGCGCTTTGACATCGAAGGATCCGATGAAGCAGACGGGAGTAAACATCTCCCAGCGGAGTC
  CAAATTCCAACAGAAACTGGCAGAAATGACTTCTACTCGAACACGAATGCAAAAGCAGAAACTGAATGAT
  AGCATGGAAATCTCAAACAAGGAGGAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217325 representing NM_017045
  Red=Cloning site Green=Tags(s)
  
  MPPKAPRRTAAAEPPPPPPPPPEDDPAQDSDPEELPLIRLEFEKIEEPEFIALCQKLKVPDHVRERAWLT
  WEKVSSVDGILEGYIQKKKELWGICIFIAAVDLDEMPFTFTELQKSIETSVYKFFDLLKEIDTSTKVDNA
  VSRLLKKYNVLCALYSKLERTCGLIYLTQPSSGLSTEINSMLVLKVSWITFLLAKGEVVQMEDDLVISFQ
  LMLCVLDYFIKLSPPALLREPYKTAATPINGSPRTPRRGQNRSARIAKQLESDTRTIEVLCKEHECNVDE
  VKNVYFKNFIPFISSLGIVSSNGLPELESLSKRYEEVYLKSKDLDARLFLDHDKTLQTDTIDSFETERTP
  RKSNPDEEANMVTPHTPVRTVMNTIQQLMVILNSASDQPSENLISYFSNCTVNPKENILKRVKDVGHIFK
  EKFASAVGQGCIDIGAQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMAT
  YSRSMLQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKVEANLTREMIKHLERCEHRIMESLAWLSDS
  PLFDLIKQSKDGEGPDHLESACSLSLPLQSNHTAADMYLSPIRSPKKRTSTTRVNSAANTETQAASAFHT
  QKPLKSTSLSLFYKKVYRLAYLRLNTLCARLLSDHPEPEHIIWTLFQHTLENEYELMKDRHLDQIMMCSM
  YGICKVKNIDLKFKIIVTAYKDLPHAAQETFKRVLIREEEFDSIIVFYNSVFMQRLKTNILQYASTRPPT
  LSPIPHIPRSPYKFSSSPLRIPGGNIYMSPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKI
  NQMVCNSDRVLKRSAEGGNPPKPLKKLRFDIEGSDEADGSKHLPAESKFQQKLAEMTSTRTRMQKQKLND
  SMEISNKEEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017045
• ORF Size: 2760 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017045.1, NP_058741.1
• RefSeq Size: 4711 bp
• RefSeq ORF: 2763 bp
• Locus ID: 24708
• UniProt ID: P33568
• Cytogenetics: 15q11
• MW: 105 kDa
• Gene Summary: plays a role in negative regulation of the cell cycle and of DNA replication; acts as a tumor suppressor [RGD, Feb 2006]