Ext1 (NM_001130540) Rat Tagged ORF Clone

CAT#: RR217161

Ext1 (myc-DDK-tagged) - Rat exostosin glycosyltransferase 1 (Ext1)

Product Images

Circular map for RR217161

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ext1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217161 representing NM_001130540
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGCCAAAAAACGCTATTTCATCCTGCTCTCAGCTGGCTCTTGTCTCGCCCTTTTGTTTTATTTTG
  GAGGCGTGCAGTTTAGGGCATCGAGGAGCCACAGCCGGAGAGAAGAGCACAGTGGTCGGAATGGCTTGCA
  CCAACCCAGTCCGGATCATTTCTGGCCCCGCTTCCCGGACGCTCTGCGCCCCTTCTTTCCTTGGGATCAA
  TTGGAAAACGAGGATTCCAGCGTGCACATTTCCCCCCGGCAGAAGCGGGACGCCAACTCGAGCATCTACA
  AAGGCAAGAAGTGCCGCATGGAGTCCTGCTTCGATTTCACCCTTTGCAAGAAAAACGGCTTCAAAGTCTA
  CGTCTACCCGCAGCAGAAAGGGGAGAAAATTGCCGAAAGTTACCAAAACATTCTAGCGGCCATCGAGGGC
  TCCAGGTTCTACACCTCGGACCCCAGCCAGGCGTGCCTCTTTGTCCTGAGTCTGGATACTTTAGACAGAG
  ACCAGTTATCACCTCAGTATGTGCACAATTTGAGATCCAAAGTGCAGAGTCTCCACTTGTGGAACAATGG
  TAGGAATCATTTAATTTTTAATTTATATTCTGGCACTTGGCCTGACTACACCGAGGACGTGGGGTTTGAC
  ATCGGCCAGGCGATGCTGGCCAAAGCCAGCATCAGTACTGAAAACTTCCGACCAAACTTTGATGTTTCTA
  TTCCCCTCTTTTCTAAGGATCATCCCAGGACAGGAGGGGAGAGGGGGTTTTTGAAATTTAACACCATCCC
  TCCCCTCAGGAAGTACATGCTGGTATTCAAGGGGAAGAGGTACCTGACAGGGATAGGGTCAGACACCAGG
  AATGCCTTATATCACGTCCATAACGGGGAGGACGTCTTGCTCCTCACCACCTGCAAGCATGGCAAAGACT
  GGCAAAAGCACAAGGATTCTCGCTGTGACAGAGACAACACCGAGTATGAGAAATATGATTATCGGGAAAT
  GCTGCACAATGCCACTTTCTGTCTGGTTCCTCGTGGTCGCAGGCTTGGGTCCTTCAGATTCCTGGAGGCT
  TTGCAGGCTGCCTGTGTCCCTGTAATGCTCAGCAATGGATGGGAGTTGCCATTCTCCGAAGTGATAAATT
  GGAACCAAGCTGCCGTCATAGGCGATGAGAGATTGCTATTACAGATTCCTTCTACAATCAGGTCTATTCA
  TCAGGATAAAATCCTAGCACTTAGACAGCAGACACAGTTCTTGTGGGAGGCTTATTTTTCTTCAGTTGAG
  AAGATTGTATTAACTACACTAGAGATTATTCAGGACAGAATATTCAAGCACATATCACGTAACAGTTTAA
  TATGGAACAAACATCCTGGAGGATTGTTCGTCCTACCACAGTATTCATCCTACCTGGGAGATTTTCCTTA
  CTACTATGCTAATTTAGGTCTAAAGCCCCCCTCCAAATTCACTGCAGTCATCCATGCTGTGACCCCCCTG
  GTCTCTCAGTCCCAGCCAGTGTTGAAGCTTCTTGTGGCAGCAGCCAAATCCCAGTACTGTGCACAGATTA
  TAGTTCTGTGGAATTGTGACAAGCCTCTACCAGCCAAACACCGCTGGCCTGCCACTGCCGTGCCTGTCAT
  CGTCATTGAAGGAGAAAGCAAGGTTATGAGCAGCCGGTTTCTGCCCTATGACAACATCATCACTGATGCT
  GTGCTCAGCCTTGATGAGGACACTGTGCTTTCAACCACGGAAGTGGATTTTGCCTTCACTGTATGGCAGA
  GCTTCCCGGAGAGGATTGTGGGATATCCTGCACGCAGCCATTTCTGGGATAACTCAAAGGAGCGATGGGG
  ATATACATCCAAGTGGACGAATGACTACTCCATGGTGTTGACAGGAGCTGCTATCTACCACAAATATTAT
  CACTACCTGTATTCCCATTACCTGCCAGCCAGCCTGAAGAACATGGTAGACCAACTGGCCAACTGTGAGG
  ACATTCTCATGAATTTCCTGGTGTCTGCTGTGACGAAATTGCCTCCAATCAAAGTGACCCAGAAGAAACA
  GTATAAGGAGACAATGATGGGACAGACTTCCCGAGCATCTCGCTGGGCTGACCCTGACCACTTTGCCCAG
  CGACAGAGCTGCATGAATACGTTTGCCAGCTGGTTTGGCTACATGCCGCTGATCCACTCTCAGATGAGGC
  TCGACCCGGTCCTCTTTAAAGACCAAGTCTCAATCCTGAGGAAGAAATACAGAGACATTGAACGACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217161 representing NM_001130540
  Red=Cloning site Green=Tags(s)
  
  MQAKKRYFILLSAGSCLALLFYFGGVQFRASRSHSRREEHSGRNGLHQPSPDHFWPRFPDALRPFFPWDQ
  LENEDSSVHISPRQKRDANSSIYKGKKCRMESCFDFTLCKKNGFKVYVYPQQKGEKIAESYQNILAAIEG
  SRFYTSDPSQACLFVLSLDTLDRDQLSPQYVHNLRSKVQSLHLWNNGRNHLIFNLYSGTWPDYTEDVGFD
  IGQAMLAKASISTENFRPNFDVSIPLFSKDHPRTGGERGFLKFNTIPPLRKYMLVFKGKRYLTGIGSDTR
  NALYHVHNGEDVLLLTTCKHGKDWQKHKDSRCDRDNTEYEKYDYREMLHNATFCLVPRGRRLGSFRFLEA
  LQAACVPVMLSNGWELPFSEVINWNQAAVIGDERLLLQIPSTIRSIHQDKILALRQQTQFLWEAYFSSVE
  KIVLTTLEIIQDRIFKHISRNSLIWNKHPGGLFVLPQYSSYLGDFPYYYANLGLKPPSKFTAVIHAVTPL
  VSQSQPVLKLLVAAAKSQYCAQIIVLWNCDKPLPAKHRWPATAVPVIVIEGESKVMSSRFLPYDNIITDA
  VLSLDEDTVLSTTEVDFAFTVWQSFPERIVGYPARSHFWDNSKERWGYTSKWTNDYSMVLTGAAIYHKYY
  HYLYSHYLPASLKNMVDQLANCEDILMNFLVSAVTKLPPIKVTQKKQYKETMMGQTSRASRWADPDHFAQ
  RQSCMNTFASWFGYMPLIHSQMRLDPVLFKDQVSILRKKYRDIERL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130540
• ORF Size: 2238 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130540.1, NP_001124012.1
• RefSeq Size: 3474 bp
• RefSeq ORF: 2241 bp
• Locus ID: 299907
• Cytogenetics: 7q31
• MW: 86.3 kDa