Sgk1 (NM_001193568) Rat Tagged ORF Clone

CAT#: RR216691

Sgk1 (myc-DDK-tagged) - Rat serum/glucocorticoid regulated kinase 1 (Sgk1), transcript variant 1

Product Images

Circular map for RR216691

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sgk1
• Synonyms: Sgk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216691 representing NM_001193568
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGAGATGCAGGGCGCGCTGGCTCGGGCTCGGCTCGAGTCCCTGCTCCGGCCCCGCCACAAAAAGC
  GAGTGGAGGCGCAGAAAAGGAGCGAGTCCGTCCTGCTAAGCGGACTGGCTTTCATGAAACAGAGAAGGAT
  GGGCCTGAACGATTTTATTCAGAAGCTTGCCAACAACTCCTATGCATGCAAACACCCTGAAGTTCAATCC
  TATTTGAAAATCTCCCAACCTCAGGAGCCCGAACTTATGAACGCCAACCCCTCACCTCCTCCAAGTCCCT
  CTCAACAAATCAACCTGGGTCCATCCTCAAATCCCCACGCCAAACCCTCTGACTTCCACTTCTTGAAAGT
  GATCGGAAAAGGCAGTTTTGGAAAGGTTCTTCTAGCAAGGCACAAGGCAGAAGAAGCATTCTATGCCGTC
  AAAGTTTTGCAGAAGAAAGCCATCTTGAAGAAGAAGGAGGAGAAGCATATTATGTCAGAGCGCAATGTTC
  TGTTGAAGAATGTGAAGCACCCTTTCCTGGTGGGCCTTCACTTCTCTTTCCAGACTGCTGACAAACTCTA
  CTTCGTCCTAGACTACATTAATGGCGGAGAGCTGTTCTACCATCTCCAGAGGGAGCGCTGCTTCCTGGAA
  CCCCGTGCTCGCTTCTACGCAGCTGAAATAGCCAGTGCCTTGGGTTATCTGCACTCCCTAAACATCGTTT
  ATCGAGACTTAAAACCAGAGAATATTCTCCTAGACTCACAGGGACACATCGTCCTCACTGACTTTGGGCT
  CTGCAAGGAGAACATCGAGCACAATGGGACAACGTCCACCTTCTGTGGCACGCCTGAGTATCTCGCTCCT
  GAGGTTCTCCATAAGCAGCCGTACGACCGGACAGTGGACTGGTGGTGCCTCGGGGCTGTCTTGTATGAGA
  TGCTCTATGGCCTGCCTCCGTTCTACAGCCGGAACACAGCCGAGATGTATGACAATATTCTGAACAAGCC
  TCTCCAGCTGAAACCAAATATCACCAACTCAGCAAGGCACCTGCTGGAGGGCCTCCTGCAGAAGGACCGG
  ACCAAGAGGCTGGGTGCCAAGGATGACTTTATGGAGATTAAGAGTCATATTTTCTTCTCTTTGATTAACT
  GGGATGATCTCATTAATAAGAAGATCACGCCCCCATTTAACCCAAATGTGAGCGGGCCCAGTGACCTTCG
  GCACTTTGATCCCGAGTTTACTGAGGAGCCGGTCCCCAGCTCCATCGGGCGATCCCCTGACAGCATCCTT
  GTCACAGCCAGTGTGAAAGAAGCCGCGGAAGCCTTCCTTGGCTTCTCCTATGCCCCTCCTATGGACTCCT
  TCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216691 representing NM_001193568
  Red=Cloning site Green=Tags(s)
  
  MGEMQGALARARLESLLRPRHKKRVEAQKRSESVLLSGLAFMKQRRMGLNDFIQKLANNSYACKHPEVQS
  YLKISQPQEPELMNANPSPPPSPSQQINLGPSSNPHAKPSDFHFLKVIGKGSFGKVLLARHKAEEAFYAV
  KVLQKKAILKKKEEKHIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRERCFLE
  PRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQGHIVLTDFGLCKENIEHNGTTSTFCGTPEYLAP
  EVLHKQPYDRTVDWWCLGAVLYEMLYGLPPFYSRNTAEMYDNILNKPLQLKPNITNSARHLLEGLLQKDR
  TKRLGAKDDFMEIKSHIFFSLINWDDLINKKITPPFNPNVSGPSDLRHFDPEFTEEPVPSSIGRSPDSIL
  VTASVKEAAEAFLGFSYAPPMDSFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001193568
• ORF Size: 1335 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001193568.1, NP_001180497.1
• RefSeq Size: 2555 bp
• RefSeq ORF: 1338 bp
• Locus ID: 29517
• Cytogenetics: 1p12
• MW: 50.6 kDa
• Gene Summary: may play a role in menory consolidation and spatial learning; may mediate aldosterone-induced signaling [RGD, Feb 2006]