Wnt3a (NM_001107005) Rat Tagged ORF Clone

CAT#: RR216470

Wnt3a (myc-DDK-tagged) - Rat wingless-type MMTV integration site family, member 3A (Wnt3a)

Product Images

Circular map for RR216470

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wnt3a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216470 representing NM_001107005
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACAACCTCTGTGTGGTGGTGGTGGTGGTGGCAGAGGTGCTAACACAGCAGCTTAATGACAGGGCAC
  TAACAAGTCGGGTTCTTCTCTGGTCCTTGGCTGTGGGACCCCAGTACTCCTCACTGAGCACTCAGCCCAT
  TCTCTGTGCCAGCATCCCGGGTCTGGTGCCCAAGCAGCTGCGCTTCTGCAGGAACTACGTGGAGATCATG
  CCCAGTGTGGCCGAGGGTGTCAAGGCGGGCATCCAAGAGTGCCAGCACCAGTTCCGAGGCCGGCGTTGGA
  ACTGCACCACTGTCAGCAACAGCCTGGCCATCTTTGGCCCCGTTCTGGACAAAGCCACCCGGGAGTCAGC
  CTTTGTCCATGCCATCGCTTCCGCTGGAGTGGCCTTCGCAGTGACCCGGTCCTGTGCAGAGGGATCAGCT
  GCCATCTGTGGGTGCAGCAGCCGCTTGCAGGGCTCCCCAGGCGAGGGCTGGAAGTGGGGTGGCTGTAGTG
  AGGACATTGAATTTGGAGGAATGGTCTCTCGGGAGTTTGCCGATGCCAGGGAGAACCGGCCGGATGCCCG
  CTCTGCCATGAACCGTCACAACAATGAGGCTGGGCGACAGGCCATCGCCAGTCACATGCACCTCAAGTGC
  AAATGCCACGGACTATCCGGCAGTTGCGAAGTGAAGACCTGCTGGTGGTCGCAGCCTGACTTCCGCACCA
  TCGGGGATTTCCTCAAGGACAAGTATGACAGCGCCTCAGAGATGGTGGTAGAGAAACACCGAGAGTCTCG
  TGGCTGGGTGGAGACCTTGAGGCCACGTTACACATACTTCAAGGTGCCCACAGAGCGCGACCTGGTCTAC
  TACGAGGCCTCACCTAACTTCTGCGAGCCCAACCCTGAAACCGGCTCCTTCGGGACGCGTGACCGCACCT
  GCAATGTGAGCTCGCATGGCATAGACGGGTGCGACCTGTTGTGCTGCGGGCGTGGGCATAACGCGCGCAC
  TGAGCGACGGAGGGAGAAATGCCACTGTGTTTTCCACTGGTGCTGTTATGTCAGCTGCCAGGAGTGCACA
  CGTGTCTATGACGTGCACACCTGCAAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216470 representing NM_001107005
  Red=Cloning site Green=Tags(s)
  
  MYNLCVVVVVVAEVLTQQLNDRALTSRVLLWSLAVGPQYSSLSTQPILCASIPGLVPKQLRFCRNYVEIM
  PSVAEGVKAGIQECQHQFRGRRWNCTTVSNSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGSA
  AICGCSSRLQGSPGEGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKC
  KCHGLSGSCEVKTCWWSQPDFRTIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVY
  YEASPNFCEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARTERRREKCHCVFHWCCYVSCQECT
  RVYDVHTCK
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107005
• ORF Size: 1077 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107005.2, NP_001100475.2
• RefSeq Size: 2642 bp
• RefSeq ORF: 1080 bp
• Locus ID: 303181
• Cytogenetics: 10q22
• MW: 40.1 kDa