Timm22 (NM_032618) Rat Tagged ORF Clone

CAT#: RR216002

Timm22 (myc-DDK-tagged) - Rat translocase of inner mitochondrial membrane 22 homolog (yeast) (Timm22)

Product Images

Circular map for RR216002

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Timm22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216002 representing NM_032618
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGCCAAGGCTGGGGCTTCAGCTCCTGAGGCGGCGGGTTCCGCCGAAGCTCCGTTGCAGTACA
  GCCTTCTTCTTCAGCACCTGGTGGGCGACAAGCGTCAGCCACGGCTTCTGGAGCCTGGCAGTCTGGGCGG
  GATCCCGAGTCCTGCCAAGAGCGAGGAGCAGAAGATGATCGAGAGGGCGATGGAAAGTTGCGCCTTCAAG
  GCTGTGCTGGCCTGTGTGGGAGGATTTGTCTTGGGAGGCGCATTTGGTGTCTTTACTGCTGGCATTGATA
  CCAACGTGGGCTTTGACCCCAAGGACCCTTACCGGACACCAACTGCAAGAGAAGTCCTGAAGGACATGGG
  ACAGAGAGGAATGTCCTATGCCAAAAATTTTGCCATTGTGGGTGCCATGTTTTCCTGTACTGAGTGTCTG
  GTAGAGTCTTACCGGGGGAAGTCAGACTGGAAGAACAGCGTCATCAGCGGCTGCATCACTGGTGGAGCCA
  TCGGCTTCAGAGCTGGAGTAAAGGCCGGGGCCATAGGTTGTGGAGGTTTTGCTGCTTTCTCTGCTGCAAT
  CGATTATTACCTACGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216002 representing NM_032618
  Red=Cloning site Green=Tags(s)
  
  MAAAKAGASAPEAAGSAEAPLQYSLLLQHLVGDKRQPRLLEPGSLGGIPSPAKSEEQKMIERAMESCAFK
  AVLACVGGFVLGGAFGVFTAGIDTNVGFDPKDPYRTPTAREVLKDMGQRGMSYAKNFAIVGAMFSCTECL
  VESYRGKSDWKNSVISGCITGGAIGFRAGVKAGAIGCGGFAAFSAAIDYYLR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032618
• ORF Size: 576 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032618.1, NP_116007.1
• RefSeq Size: 1801 bp
• RefSeq ORF: 579 bp
• Locus ID: 79463
• UniProt ID: Q9JKW1
• Cytogenetics: 10q24
• MW: 19.9 kDa
• Gene Summary: controls the import of integral inner membrane proteins without a matrix targeting signal into the mitochondria [RGD, Feb 2006]