Bicra (NM_001106226) Rat Tagged ORF Clone

CAT#: RR215535

Gltscr1 (Myc-DDK-tagged ORF) - Rat glioma tumor suppressor candidate region gene 1 (Gltscr1), (10 ug)

Product Images

Circular map for RR215535

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bicra
• Synonyms: Gltscr1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215535 representing NM_001106226
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGATGAGGATGGGAGATGCTTACTAGACGTGATTTGTGACCCTCAGGCCCTCAATGACTTCTTGC
  ATGGATCCGAAAAGCTGGACAGTGATGACCTCCTGGATGCCCCTGTGGAGGCCCAAAGTGCCTTCTATGA
  AGGTCCTGGGCTCCATGTGCAGGAAGCTGCCGGCAACCACCTGAACCCTGAGCCCAGCCAACCTGCCCCC
  AGCGTGGACCTGGACTTCCTAGAAGATGATATCCTGGGCTCCCCTGCAGCAGGAGGAGGTGGAGGGGGCG
  GCGGGGGCCCAGACCAGCCCTGTGACATTCTTCAGCAGAGTCTTCAGGAGGCCAACATCACAGAGCAGAC
  CCTGGAGGCTGAGGCTGAACTGGACCTGGGCCCCTTCCAGCTGCCCACCCTACAGCCCGCTGACGGCGGG
  GCAGGTGCTACTGGAGCTGCAGGAGCTGCTGCAGTGACCGCAGGGCCCCAGGCTCTCTTCCCAGGCAGCG
  CAGATCTGCTGGGGCTGCAAGCCCCGCCCACTGTACTTACCCACCAGGCCTTGGTGCCACCCCAGGATGT
  GGTCAACAAGGCCCTGAGCGTCCAGCCCTTCCTACAGCCCGTGGGCCTGGGCAATGTGACCCTTCAGCCC
  ATTCAAGGCCTCCAGGGCCTTCCCAATGGCAGTCCTGGGGGCGCTACAGCAGCCACTTTGGGTCTGGCAC
  CTATTCAAGTGGTGGGCCAGCCCGTCATGGCTCTCAATCCACCCACCTCCCAGCTCTTGGCAAAGCAGGT
  GCCTGTCAGTGGCTACCTGGCCTCCGCAGCTGGTCCTTCAGAGCCAGTGACACTGGCATCTGCCGGCGTG
  TCCCCCCAGGGAGCCGGCCTAGTCATCCAGAAAAATCTTCCAGCCGCAGTGACCACCACACTCAACGGGA
  ACTCAGTCTTTGCCGGGACAGGGTCTGCCACTGCAGCAGCCAGTGGGGCACCCTCGGGACAGCCGCTGGC
  GGTGGCCCCGGGCCTTGGCACGTCACCACTGGTACAAGCACCCAATGTGATTTTACACAGAACCCCTACG
  CCCATCCAGCCCAAGCCTGCAGGGGTACTGCCCCCCAAACTCTACCAGCTGACCCCCAAGCCCTTCACCC
  CCACGGGAGCCACCCTTACCATCCAGGGTGAGCCAGGCACCTTGCCCCAGCAGCCTAAGGCCCCCCAGAA
  CCTGACTTTTATGGCCGCAGGCAAAGCTGGTCAGAACGTGGTGCTGTCTAGCTTCCCAGCACCGGCTTTG
  CAGGCGAATGTATTCAAGCAGCCACCAGTCACCACCACGGGGACAGCCCCGCCACAGCCACCCGGGGCCC
  TCAGCAAACCCATGAGCGTCCACCTCCTCAATCAAGGCAGTAGCATCGTGATCCCAGCCCAGCACATGCT
  GCCTGGCCAGAACCAGTTCTTGCTGCCAGGCACCTCGGCTGTACAACTACCCCAGTCACTTTCTGCCCTG
  CCTGCCAACGTGGGAGGCCAGATCCTCACAGCCGCAGCACCACATGCAGGTGGACAGCTCATTGCCAACC
  CAATCCTCACCAACCAGAACCTGGCAGGCCCACTAAGTCTGGGCCCAGTGCTGGCACCCCACTCTGGGGC
  ACACAGCGCTGCGCACATCCTCTCTGCAGCTCCCATCCAGGTGGGCCAGCCTGCCCTCTTCCAGATGCCT
  GTGTCGCTGGCCACTGGCAGCCTGCCCACTCAGAGCCAGCCAGCTCCCACTGGCCCCACAGCCACCACCG
  TCCTCCAGGGTGTCACCCTGCCTCCCAGTGCTGTGGCCATGCTTAACACACCTGATGGGCTAGTGCAGCC
  CTCAACCCCAGCTGCCACCACTGGGGAAGCCACACCTGTTCTGGCCGTTCAACCTGCCACCCAGGTACCC
  CCTGCTGTCACCACACCACTGCCTATGGGTCTCCAACAGTCACCGGCACAGCAGCCTCCACAGGCCCCTA
  CTCCACAGGCGGCCACCCAGCCTCAGGCCACCCCTCCTCAGGCCAGTCCAGGCCTGGCATCCAGCCCAGA
  GAAGATAGTCCTGGGGCAGGCGCCCCCTGCGGCCACAACGGCCATCCTCACTCAGGATTCCCTCCAGATG
  TTCCTGCCCCAGGAGAGGAGCCAGCAGCCCCTCTCTACAGAGGGTCCCCACCTCTCGGTGCCTGCCTCCG
  TCATAGTCAGCGCCCCGCCTCCTGCCCAAGACCCAGCCCTGGCCACGCCCGTCACCAAAGGAGCTGGCCT
  TGGCGCTCAGACCCCCGACAACCGGGCTTCCCCAGCTCCAGCTCCCCAGATCCCAGCAGCTGCTCCACTG
  AAAGCCCCTGGCCCTGCCTCCTCCCCATCACTACCTCACCAGGCCCCCCTGGGAGACAGTCCCCACATGC
  CCTCCCCACACCCTGCCAGGCCCCCTTCCCGCCCACCCTCGAGACCCCACTCACGCCCTCCATCCCAGCC
  CCAGAGCCTGACCTGCCCACCCTCCGAGCCCACCCTGCACCCTTGCCCTCCACCCCAGGGTCCCCCAACT
  CTACCTGGCATCTTTGTCATCCAGAATCAATTGGGTGCCCCACCACCAGCCAGCACCCCAGCCCCCACAG
  CCCCAGGCCCACCCCAGCCCCCTCTGCGACCCCCATCCCAGCCTCCTGAGGGCCCATTGCCCCCAGCCTC
  CCACCTCCCTCCTGCCTCCACCCCTTCCGCTGTGGCCTCCTCCTCTGAGCCTTCTACCAGGTTGCCAGTC
  CCCACACCCCCTGACTTCCAGTTCCCACCAGGCCAGGGACCCCATAAGTCCCCCACTCCACCACCAGCCC
  TCCATTTGGTCCCTGAGCCCACGGCACCCCCTCCTCCACCACCTCGGACCTTCCAGATGGTAACTGCCCC
  CTTCCCAGCGCTGCCCCAGCCAAAAGCACTTCTGGAACGATTCCACCAGGTGCCATCTGGGATTATTCTC
  CAGAATAAGGCTGGGGGTACTCCCACCACCCCACAGACATCCACCACCCTGGGGACCCTCACCAGTCCTA
  CTGCCTCTGTGCTAGTCAGTGGACAGGCACCACCTGGGACTCCTGCTGCCTCTAGCCATGCCCCAGCCTC
  CACACCTATGGCCACCACAGGTCTCCCTCCTCTACTTCCTGCCGAAAACAAAGCTTTTGCCAGCAACCTT
  CCAACCCTGAGTGTGGCCAAAGCTACTGTGTCTGGGCCAGGGAAGTCCCCAGCGATGCAGTATGACAGCA
  AGTTGGGTAGCCTGAAGAAACAGCCCCTTCTGCAACCCAGCAAAGAAGCCTGCTTCCTGGAGCATCTGCA
  CAAACATCAGGGTTCTGTCCTGCACCCTGATTACAAGACAGCTTTCCCCTCCTTTGAGGACGCCCTCCAT
  CGCCTCCTGCCCTACCATGTCTACCAAGGCGCCCTCCCCTCCCCCAATGACTACCATAAAGTGGATGAAG
  AATTTGAGACTGTCTCTACGCAGCTGCTCAAACGTACCCAGGCCATGCTCAATAAATATCGGCTTTTGCT
  TCTGGAAGAGTCCAGGAGAGTCAGTCCTTCCGCAGAGATGGTTATGATCGACCGAATGTTCATTCAGGAG
  GAGAAGACCACCCTTGCCTTGGATAAGCAGCTCGCCAAGGAGAAACCTGATGAGTATGTGTCTTCCTCCC
  GCTCCCTTGGCTTCCCTGTCCCAGTGTCTTCCGAGGGTCACCGGCTCCCCAGCCACGGCCCATCTTCTTC
  ATCCTCCACATCTGGAACATCTGCCCAGCCCCCTCCTCACCCGCCCACCAAGCTAGTGATCCGGCATGGC
  GGAGCTGGCGGCTCCCCCTCAGTGACCTGGGCCCGGGCATCATCCTCCTTGTCATCAACGTCCTCATCCT
  CATCCTCATCCTCTGTCGCCTCATCCCTGGACGCAGATGAGGACGGCCCCATGCCCTCCCGTAGCCGGCC
  ACCCATTAAGACCTATGAGGCCCGGAGCCGCATTGGCCTCAAACTCAAGATCAAGCAGGAGGCGGGGCTC
  AGCAAGGTGGTGCACAACACTGCATTGGATCCTGTACACCAGCCCTTGCCTGCTCCAACCCCTGCAAAAG
  GGGCTGAACCTCCGCCACACCCAGCTCCTCCCCCACTCCCACCTGCCACCCAGGCACAGATGAATGGCAC
  TCTGGACCATCCCCCACCCATAGTACGCAAACCCACGGTGCCTGCGTCCTGCCCACGTCTACCACTGCGC
  AAGACCTACCGAGAAAACATGGGCGATCCTAGTGCCACTGAGGGTGCACAGGGACGGCCGCGGGGTGCGG
  GCAGCCCCACCCCACTGCCCACCAAGGTAGACGAAGCCACCAGTGGGCTGATCCGGGAGCTGGCAGCGGT
  GGAGGACGAATTGTATCAGCGGGTTCTGAAGGGCGGCCCACCACCCCCGGAGACCCCAGCCTCTGCTACT
  AGCCAAGGCCCCACAGAATCTGGTTGGGAAGCACCCATGCTACCCCCAACCAAACGACGCAAGTCTGAGT
  CCCCGGACGTGGACCAGGCCAGCTTCTCTAGTGACAGCCCGCAGGATGATACACTCACTGAGCATTTGCA
  GAGTGCCATCGACAGCATCCTTAACCTGCAGCAGGCCCCCGGCCGGACACCTGCAGGCCCATACCCCCAT
  ACGGGCCCCACGCCTGGCACCCCCACATCCCCAGCTCCTCTGCACAGGCCTGACGCCTTCCCACCCTCTA
  GTCACAATGGTGGCCTCGGCGCCAGGACGTTGAACAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215535 representing NM_001106226
  Red=Cloning site Green=Tags(s)
  
  MDDEDGRCLLDVICDPQALNDFLHGSEKLDSDDLLDAPVEAQSAFYEGPGLHVQEAAGNHLNPEPSQPAP
  SVDLDFLEDDILGSPAAGGGGGGGGGPDQPCDILQQSLQEANITEQTLEAEAELDLGPFQLPTLQPADGG
  AGATGAAGAAAVTAGPQALFPGSADLLGLQAPPTVLTHQALVPPQDVVNKALSVQPFLQPVGLGNVTLQP
  IQGLQGLPNGSPGGATAATLGLAPIQVVGQPVMALNPPTSQLLAKQVPVSGYLASAAGPSEPVTLASAGV
  SPQGAGLVIQKNLPAAVTTTLNGNSVFAGTGSATAAASGAPSGQPLAVAPGLGTSPLVQAPNVILHRTPT
  PIQPKPAGVLPPKLYQLTPKPFTPTGATLTIQGEPGTLPQQPKAPQNLTFMAAGKAGQNVVLSSFPAPAL
  QANVFKQPPVTTTGTAPPQPPGALSKPMSVHLLNQGSSIVIPAQHMLPGQNQFLLPGTSAVQLPQSLSAL
  PANVGGQILTAAAPHAGGQLIANPILTNQNLAGPLSLGPVLAPHSGAHSAAHILSAAPIQVGQPALFQMP
  VSLATGSLPTQSQPAPTGPTATTVLQGVTLPPSAVAMLNTPDGLVQPSTPAATTGEATPVLAVQPATQVP
  PAVTTPLPMGLQQSPAQQPPQAPTPQAATQPQATPPQASPGLASSPEKIVLGQAPPAATTAILTQDSLQM
  FLPQERSQQPLSTEGPHLSVPASVIVSAPPPAQDPALATPVTKGAGLGAQTPDNRASPAPAPQIPAAAPL
  KAPGPASSPSLPHQAPLGDSPHMPSPHPARPPSRPPSRPHSRPPSQPQSLTCPPSEPTLHPCPPPQGPPT
  LPGIFVIQNQLGAPPPASTPAPTAPGPPQPPLRPPSQPPEGPLPPASHLPPASTPSAVASSSEPSTRLPV
  PTPPDFQFPPGQGPHKSPTPPPALHLVPEPTAPPPPPPRTFQMVTAPFPALPQPKALLERFHQVPSGIIL
  QNKAGGTPTTPQTSTTLGTLTSPTASVLVSGQAPPGTPAASSHAPASTPMATTGLPPLLPAENKAFASNL
  PTLSVAKATVSGPGKSPAMQYDSKLGSLKKQPLLQPSKEACFLEHLHKHQGSVLHPDYKTAFPSFEDALH
  RLLPYHVYQGALPSPNDYHKVDEEFETVSTQLLKRTQAMLNKYRLLLLEESRRVSPSAEMVMIDRMFIQE
  EKTTLALDKQLAKEKPDEYVSSSRSLGFPVPVSSEGHRLPSHGPSSSSSTSGTSAQPPPHPPTKLVIRHG
  GAGGSPSVTWARASSSLSSTSSSSSSSSVASSLDADEDGPMPSRSRPPIKTYEARSRIGLKLKIKQEAGL
  SKVVHNTALDPVHQPLPAPTPAKGAEPPPHPAPPPLPPATQAQMNGTLDHPPPIVRKPTVPASCPRLPLR
  KTYRENMGDPSATEGAQGRPRGAGSPTPLPTKVDEATSGLIRELAAVEDELYQRVLKGGPPPPETPASAT
  SQGPTESGWEAPMLPPTKRRKSESPDVDQASFSSDSPQDDTLTEHLQSAIDSILNLQQAPGRTPAGPYPH
  TGPTPGTPTSPAPLHRPDAFPPSSHNGGLGARTLNR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106226
• ORF Size: 4728 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001106226.2, NP_001099696.2
• RefSeq Size: 5516 bp
• RefSeq ORF: 4731 bp
• Locus ID: 292622
• Cytogenetics: 1q21
• MW: 160.9 kDa