Supt16h (NM_001107261) Rat Tagged ORF Clone

CAT#: RR215258

Supt16h (Myc-DDK-tagged ORF) - Rat suppressor of Ty 16 homolog (S. cerevisiae) (Supt16h), (10 ug)

Product Images

Circular map for RR215258

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Supt16h
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215258 representing NM_001107261
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTGACTCTGGACAAAGACGCGTACTACCGGCGAGTTAAGAGACTGTACAGTAACTGGCGGAAAG
  GAGAAGATGAGTATGCCAGTATTGATGCCATTGTTGTATCGGTGGGTGTTGATGAAGAAATTGTATATGC
  CAAGTCAACTGCCTTACAGACGTGGCTCTTTGGTTATGAACTAACTGATACAATCATGGTCTTCTGTGAT
  GACAAAATCATCTTCATGGCCAGCAAAAAGAAGGTGGAGTTTCTGAAACAGATTGCCAATACTAAAGGCA
  ATGAGAATGCTAATGGAGCTCCTGCCATCACACTGCTTGTCAGAGAAAAGAATGAAAGTAATAAGAGCAG
  CTTTGACAAAATGATTGAAGCCATTAAAGAAAGCAAGAACGGCAAGAAAATTGGAGTGTTCAGCAAAGAC
  AAGTTCCCTGGAGAATTCATGAAGAGCTGGAGTGACTGTCTTAACAAGGAGGGCTTTGACAAAGTAGACA
  TCAGCGCTGTTGTGGCATACACTATTGCTGTGAAAGAGGATGGTGAGCTCAACCTGATGAAGAAAGCAGC
  CAGCATTACCTCTGAGGTCTTCAACAAGTTCTTCAAGGAAAGAGTCATGGAAATAGTGGATGCAGATGAG
  AAAGTTCGGCATAGCAAATTGGCTGAGTCTGTGGAAAAGGCCATCGAAGAGAAAAAATACCTAGCCGGGG
  CAGACCCTTCTACTGTGGAAATGTGTTACCCTCCTATCATTCAGAGTGGTGGCAACTACAATCTCAAGTT
  CAGTGTGGTGAGTGATAAAAATCATATGCATTTTGGAGCCATTACTTGTGCCATGGGCATTCGCTTTAAA
  TCATACTGCTCCAACCTTGTTCGCACTCTGATGGTTGACCCTACTCAGGAAGTTCAAGAAAATTACAACT
  TTTTACTTCAACTTCAAGAGGAGTTGCTAAAGGAATTAAGACATGGTGTGAAGATATGTGATGTGTATAA
  CTCTGTCATGGATGTGGTTAAGAAGCAGAAGCCAGAACTGCTGAACAAAATTACAAAAAACCTAGGATTT
  GGGATGGGAATTGAATTCCGTGAAGGCTCTCTAGTGATCAATAGTAAAAATCAGTACAAGCTAAAGAAAG
  GGATGGTTTTCAGCATCAACCTAGGATTTTCAGACCTGACTAACAAAGAAGGGAAAAAACCAGAAGAGAA
  AACCTATGCCCTGTTTATTGGTGACACTGTGCTTGTAGATGAGGATGGTCCAGCCACTGTCCTTACATCT
  GTGAAGAAGAAGGTAAAGAACGTGGGCATTTTCCTCAAGAATGAGGACGATGAGGAGGAGGAGGAGGAGA
  AAGATGAGGCGGAGGACCTTTTGGGAAGAGGTTCTAGAGCAGCGTTACTTACTGAAAGAACACGGAATGA
  GATGACTGCAGAAGAGAAGCGAAGAGCCCATCAGAAGGAACTGGCAGCACAGCTCAACGAGGAAGCGAAG
  AGGAGGCTGACAGAGCAGAAAGGGGAACAGCAGATTCAGAAGGCTCGAAAATCTAATGTGTCCTATAAAA
  ACCCATCTCTGATGCCTAAGGAACCACATATTCGGGAAATGAAGATCTATATTGATAAGAAATACGAGAC
  TGTGATAATGCCTGTGTTTGGCATTGCCACACCCTTCCATATTGCCACAATCAAGAACATAAGTATGTCT
  GTCGAAGGAGACTATACTTACTTGCGAATCAACTTCTATTGTCCAGGAAGTGCTCTGGGCAGGAACGAGG
  GCAACATCTTTCCTAACCCTGAAGCTACTTTTGTCAAGGAAATTACATACCGAGCTTCAAATATGAAAGC
  ACCTGGGGAGCAGACTGTACCCGCCTTAAATCTTCAGAATGCTTTTCGAATTATAAAAGAAGTGCAGAAA
  CGTTACAAGACCAGAGAAGCTGAAGAGAAAGAAAAAGAGGGCATTGTAAAACAAGACTCACTGGTGATCA
  ACCTAAACAGAAGTAATCCAAAGCTGAAAGATCTATACATTCGCCCAAACATTGCCCAAAAGAGAATGCA
  AGGCTCCCTGGAGGCTCACGTCAATGGTTTCCGCTTCACATCTGTTCGAGGAGACAAGGTGGATATTTTG
  TACAATAATATCAAGCACGCACTCTTCCAGCCCTGTGATGGCGAGATGATTATTGTTTTGCACTTTCACC
  TCAAGAATGCTATCATGTTCGGGAAGAAGCGGCACACAGACGTACAGTTCTACACAGAAGTTGGAGAGAT
  CACTACAGATTTGGGGAAACATCAACACATGCATGACCGAGATGACCTATATGCTGAGCAGATGGAAAGA
  GAGATGAGACACAAACTGAAAACAGCCTTTAAGAACTTCATTGAAAAAGTAGAGGCTCTAACAAAGGAGG
  AACTGGAGTTTGAAGTACCATTCAGGGATCTGGGGTTTAATGGGGCTCCCTACAGGAGTACCTGCCTCCT
  TCAGCCCACTAGTAGTGCACTGGTAAATGCTACAGAGTGGCCACCCTTTGTGGTGACACTGGATGAAGTG
  GAGCTGATCCACTTTGAGAGGGTCCAATTTCACCTGAAGAACTTTGACATGGTGATTGTCTACAAGGATT
  ACAGCAAGAAAGTCACGATGATCAACGCCATTCCTGTTGCCTCTCTAGACCCCATCAAGGAGTGGCTAAA
  TTCCTGTGACCTAAAGTACACAGAAGGTGTTCAGTCCCTCAACTGGACTAAAATCATGAAGACCATCGTT
  GACGACCCTGAAGGCTTCTTTGAACAAGGTGGCTGGTCTTTCCTGGAACCTGAGGGTGAGGGCAGTGATG
  CTGAGGACGGGGACTCGGAGTCTGAAATTGAAGATGAGACCTTCAATCCTTCCGAGGATGACTATGAAGA
  GGAAGAAGAGGACAGTGATGAAGACTATTCATCAGAAGCTGAAGAATCAGATTATTCTAAGGAATCTCTG
  GGAAGTGAAGAAGAAAGCGGGAAAGACTGGGATGAGCTAGAAGAAGAAGCACGAAAAGCGGACCGAGAAA
  GCCGTTATGAGGAGGAGGAAGAACAGAGCCGAAGTATGAGCCGGAAGAGGAAGGCGTCTGTACATAGTTC
  AGGCCGTGGCTCTAACCGCGGTTCCAGACACAGCTCTGCACCCCCGAAGAAAAAGAGAAAG
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215258 representing NM_001107261
  Red=Cloning site Green=Tags(s)
  
  MAVTLDKDAYYRRVKRLYSNWRKGEDEYASIDAIVVSVGVDEEIVYAKSTALQTWLFGYELTDTIMVFCD
  DKIIFMASKKKVEFLKQIANTKGNENANGAPAITLLVREKNESNKSSFDKMIEAIKESKNGKKIGVFSKD
  KFPGEFMKSWSDCLNKEGFDKVDISAVVAYTIAVKEDGELNLMKKAASITSEVFNKFFKERVMEIVDADE
  KVRHSKLAESVEKAIEEKKYLAGADPSTVEMCYPPIIQSGGNYNLKFSVVSDKNHMHFGAITCAMGIRFK
  SYCSNLVRTLMVDPTQEVQENYNFLLQLQEELLKELRHGVKICDVYNSVMDVVKKQKPELLNKITKNLGF
  GMGIEFREGSLVINSKNQYKLKKGMVFSINLGFSDLTNKEGKKPEEKTYALFIGDTVLVDEDGPATVLTS
  VKKKVKNVGIFLKNEDDEEEEEEKDEAEDLLGRGSRAALLTERTRNEMTAEEKRRAHQKELAAQLNEEAK
  RRLTEQKGEQQIQKARKSNVSYKNPSLMPKEPHIREMKIYIDKKYETVIMPVFGIATPFHIATIKNISMS
  VEGDYTYLRINFYCPGSALGRNEGNIFPNPEATFVKEITYRASNMKAPGEQTVPALNLQNAFRIIKEVQK
  RYKTREAEEKEKEGIVKQDSLVINLNRSNPKLKDLYIRPNIAQKRMQGSLEAHVNGFRFTSVRGDKVDIL
  YNNIKHALFQPCDGEMIIVLHFHLKNAIMFGKKRHTDVQFYTEVGEITTDLGKHQHMHDRDDLYAEQMER
  EMRHKLKTAFKNFIEKVEALTKEELEFEVPFRDLGFNGAPYRSTCLLQPTSSALVNATEWPPFVVTLDEV
  ELIHFERVQFHLKNFDMVIVYKDYSKKVTMINAIPVASLDPIKEWLNSCDLKYTEGVQSLNWTKIMKTIV
  DDPEGFFEQGGWSFLEPEGEGSDAEDGDSESEIEDETFNPSEDDYEEEEEDSDEDYSSEAEESDYSKESL
  GSEEESGKDWDELEEEARKADRESRYEEEEEQSRSMSRKRKASVHSSGRGSNRGSRHSSAPPKKKRK
  
  TRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107261
• ORF Size: 3141 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107261.1, NP_001100731.1
• RefSeq Size: 4455 bp
• RefSeq ORF: 3144 bp
• Locus ID: 305851
• Cytogenetics: 15p14
• MW: 119.9 kDa