Aldh1a1 (NM_022407) Rat Tagged ORF Clone

CAT#: RR215183

Aldh1a1 (Myc-DDK-tagged ORF) - Rat aldehyde dehydrogenase 1 family, member A1 (Aldh1a1), (10 ug)

Product Images

Circular map for RR215183

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aldh1a1
• Synonyms: Ahd2; Aldh1; Aldh2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215183 representing NM_022407
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCCCCTGCACAGCCTGCAGTTCCTGCCCCACTGGCCAACTTGAAGATTCAACACACCAAGATCT
  TCATAAACAATGAATGGCATGATTCAGTGAGTGGCAAGAAATTTCCTGTCCTTAACCCTGCAACTGAGGA
  GGTCATCTGCCATGTGGAAGAAGGGGACAAGGCAGATGTTGACAAAGCTGTGAAGGCCGCAAGACAGGCT
  TTCCAGATTGGCTCCCCATGGCGCACCATGGATGCTTCAGAGAGAGGACGCTTGCTGAACAAGCTGGCTG
  ACTTAATGGAGAGAGATCGTCTGCTGCTGGCTACAATAGAGGCCATCAATGGTGGGAAAGTCTTTGCCAA
  TGCGTACTTGTCGGATTTAGGAGGCAGCATAAAAGCATTAAAGTACTGTGCAGGCTGGGCTGACAAGATC
  CATGGTCAAACAATACCGAGTGATGGAGACATTTTCACTTTTACGAGACGTGAACCTATTGGGGTGTGTG
  GCCAAATCATCCCTTGGAATTTTCCACTGCTTATGTTCATTTGGAAGATAGGCCCTGCCCTCAGCTGTGG
  GAACACGGTGGTCGTCAAGCCAGCAGAGCAAACTCCTCTCACTGCTCTTCACATGGCATCTTTAATAAAA
  GAGGCAGGGTTTCCTCCTGGCGTGGTGAACATTGTCCCTGGTTATGGGCCAACTGCGGGGGCAGCGATCT
  CCTCTCACATGGATGTGGACAAGGTGGCCTTCACGGGATCAACCCAGGTTGGCAAATTAATCAAGGAAGC
  TGCAGGAAAAAGCAATCTGAAGAGGGTCACGCTGGAGCTTGGGGGAAAGAGCCCTTGCATTGTGTTTGCA
  GATGCCGACTTGGACATTGCTGTCGAGTTTGCACACCATGGAGTCTTCTACCATCAAGGCCAATGCTGCG
  TCGCGGCATCCCGGATTTTCGTTGAGGAGTCAGTTTATGATGAGTTTGTGAGAAAGAGTGTTGAGCGAGC
  CAAGAAATATGTCCTTGGAAACCCTCTGACCCAAGGAATAAATCAGGGCCCTCAGATTGACAAGGAGCAA
  CATGATAAAATCCTTGATCTCATTGAGAGTGGGAAGAAAGAAGGAGCCAAACTGGAGTGTGGTGGAGGAC
  GCTGGGGGAACAAAGGCTTCTTTGTCCAGCCCACAGTCTTCTCCAATGTGACCGATGAGATGCGCATTGC
  CAAAGAGGAGATATTTGGACCAGTGCAACAAATCATGAAGTTTAAGTCCATAGATGATGTGATCAAGAGA
  GCAAACAATACTACCTATGGTCTAGCAGCAGGAGTCTTCACAAAAGACCTGGACAGGGCCATCACTGTGT
  CTTCTGCTCTGCAGGCCGGGGTAGTGTGGGTTAACTGCTATATGATCTTGTCAGCCCAGTGCCCCTTCGG
  TGGATTCAAGATGTCTGGAAATGGACGAGAACTGGGTGAACATGGTCTTTATGAGTACACTGAGCTCAAG
  ACAGTTGCAATGAAAATATCTCAGAAGAACTCCACAGTTGCAATGAAAATATCTCAGAAGAACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215183 representing NM_022407
  Red=Cloning site Green=Tags(s)
  
  MSSPAQPAVPAPLANLKIQHTKIFINNEWHDSVSGKKFPVLNPATEEVICHVEEGDKADVDKAVKAARQA
  FQIGSPWRTMDASERGRLLNKLADLMERDRLLLATIEAINGGKVFANAYLSDLGGSIKALKYCAGWADKI
  HGQTIPSDGDIFTFTRREPIGVCGQIIPWNFPLLMFIWKIGPALSCGNTVVVKPAEQTPLTALHMASLIK
  EAGFPPGVVNIVPGYGPTAGAAISSHMDVDKVAFTGSTQVGKLIKEAAGKSNLKRVTLELGGKSPCIVFA
  DADLDIAVEFAHHGVFYHQGQCCVAASRIFVEESVYDEFVRKSVERAKKYVLGNPLTQGINQGPQIDKEQ
  HDKILDLIESGKKEGAKLECGGGRWGNKGFFVQPTVFSNVTDEMRIAKEEIFGPVQQIMKFKSIDDVIKR
  ANNTTYGLAAGVFTKDLDRAITVSSALQAGVVWVNCYMILSAQCPFGGFKMSGNGRELGEHGLYEYTELK
  TVAMKISQKNSTVAMKISQKNS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022407
• ORF Size: 1503 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2095 bp
• RefSeq ORF: 1506 bp
• Locus ID: 24188
• UniProt ID: P51647
• Cytogenetics: 1q51
• MW: 54.5 kDa
• Gene Summary: ubiquitous enzyme located in virtually all mammalian tissues; catalyzes oxidation of aldehyde substrates to carboxylic acids; detoxifies ethanol-derived acetaldehyde [RGD, Feb 2006]