Sts (NM_012661) Rat Tagged ORF Clone

CAT#: RR215177

  • TrueORF®

Sts (Myc-DDK-tagged ORF) - Rat steroid sulfatase (Sts), (10 ug)

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_012661" in other vectors (3)

Reconstitution Protocol

USD 591.00

5 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

USD 198.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Sts
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR215177 representing NM_012661
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCTGGCCCTGTCTCCTTGCCCTGCTCCTCTCCCAGCTCAACTTTCTCTGTGCTGCCCGTCCTGGCC
CTGGACCTAACTTCCTGCTCATCATGGCTGATGACCTTGGCATTGGGGACCTAGGCTGCTATGGCAACCG
CACACTCAGGACGCCACACATCGACCGCCTGGCCCTTGAGGGGGTGAAGTTGACTCAGCACCTGGCGGCT
GCGCCCCTGTGCACCCCGAGCCGCGCTGCCTTCCTTACCGGGCGCTACCCTGTGCGGTCAGGGATGGCAT
CACATGGCCGCCTGGGTGTATTCCTGTTCTCGGCATCATCTGGGGGGCTCCCGCCCAATGAAGTCACCTT
TGCAAAGTTGCTCAAGGGTCAAGGCTACACCACCGGGCTTGTAGGGAAGTGGCATCTGGGGCTGAGCTGC
CAAGCAGCCTCTGACTTCTGCCACCACCCTGGGCGCCATGGCTTTGACCGCTTCCTGGGCACGCCCACCA
CCAACCTGAGGGACTGCAAGCCAGGGGGCGGGACCGTGTTTGGCTCTGCCCAACAAGTATTCGTGGTCCT
GCCCATGAATATTCTGGGGGCAGTGCTGCTGGCCATGGCACTTGCGCGGTGGGCAGGGCTGGCCCGGCCC
CCAGGGTGGGTGTTCGGGGTGACAGTGGCTGCCATGGCGGCGGTGGGCGGGGCCTATGTGGCCTTCCTCT
ACCACTTCCGCCCAGCCAACTGCTTCCTCATGGCCGACTTCACCATCACCCAACAGCCCACGGACTACAA
GGGGCTCACACAGCGCCTGGCAAGCGAGGCTGGGGACTTCCTGCGCAGGAACAGGGACACGCCCTTCCTG
CTATTCCTGTCTTTCATGCATGTGCACACGGCACACTTCGCCAACCCTGAGTTTGCCGGGCAGAGCCTGC
ATGGGGCCTATGGAGATGCTGTGGAGGAGATGGACTGGGCCGTAGGCCAGGTCCTTGCCACACTGGACAA
GCTGGGCCTCGCCAACAACACACTCGTGTACTTGACGTCCGACCATGGCGCACACGTGGAGGAGCTTGGC
CCCAACGGTGAACGGCATGGCGGGAGCAACGGAATTTACAGGGGCGGGAAGGCCAACACGTGGGAGGGTG
GCATCCGTGTGCCTGGACTTGTGCGCTGGCCCGGCGTGATTGTGCCCGGCCAGGAGGTCGAGGAGCCCAC
GAGCAACATGGACGTCTTCCCCACTGTGGCCAGGCTTGCAGGGGCAGAGCTGCCGACTGACAGGGTGATT
GACGGGCGTGACTTGATGCCCCTGCTGCTGGGCCATGTACAACACTCTGAGCATGAATTCCTGTTCCACT
ACTGCAATGCCTACCTCAGCGCTGTGGCATGGCGGCCCCACAACAGCTCGTCCGTGTGGAAGGCGTTTTA
CTTCACACCGAACTTCGACCCCCCGGGCTCTAATGGCTGCTTCTCCACACACGTGTGCATGTGCCACGGC
CACCATGTCACGCACCATGATCCGCCCCTGCTCTTCGACATTGCCCGTGACCCCAGGGAGCGCCACCCGC
TCACGCCTGAAACAGAGCCGCGCCATGGTGAGATCCTGCGCAACATGGATGCTGCGGCCCGTGCACACGT
TGCTACCCTAGAGGAGGCGCCCAATCAGCTTTCCATGTCAAATGTCGCATGGAAGCCATGGCTGCAGCTC
TGCCTGCCTTCCAAGCCCCACCCACTCGCATGCCGCTGTGCTGGCGACGGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR215177 representing NM_012661
Red=Cloning site Green=Tags(s)

MLWPCLLALLLSQLNFLCAARPGPGPNFLLIMADDLGIGDLGCYGNRTLRTPHIDRLALEGVKLTQHLAA
APLCTPSRAAFLTGRYPVRSGMASHGRLGVFLFSASSGGLPPNEVTFAKLLKGQGYTTGLVGKWHLGLSC
QAASDFCHHPGRHGFDRFLGTPTTNLRDCKPGGGTVFGSAQQVFVVLPMNILGAVLLAMALARWAGLARP
PGWVFGVTVAAMAAVGGAYVAFLYHFRPANCFLMADFTITQQPTDYKGLTQRLASEAGDFLRRNRDTPFL
LFLSFMHVHTAHFANPEFAGQSLHGAYGDAVEEMDWAVGQVLATLDKLGLANNTLVYLTSDHGAHVEELG
PNGERHGGSNGIYRGGKANTWEGGIRVPGLVRWPGVIVPGQEVEEPTSNMDVFPTVARLAGAELPTDRVI
DGRDLMPLLLGHVQHSEHEFLFHYCNAYLSAVAWRPHNSSSVWKAFYFTPNFDPPGSNGCFSTHVCMCHG
HHVTHHDPPLLFDIARDPRERHPLTPETEPRHGEILRNMDAAARAHVATLEEAPNQLSMSNVAWKPWLQL
CLPSKPHPLACRCAGDG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_012661
ORF Size 1731 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_012661.1, NP_036793.1
RefSeq Size 2472 bp
RefSeq ORF 1734 bp
Locus ID 24800
UniProt ID P15589
Cytogenetics Xq21
MW 62.7 kDa
Gene Summary enzyme that converts sulfated precursors to estrogen during pregnancy [RGD, Feb 2006]

Other Versions

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.