Rnf146 (NM_001012060) Rat Tagged ORF Clone

CAT#: RR214781

Rnf146 (Myc-DDK-tagged ORF) - Rat ring finger protein 146 (Rnf146), (10 ug)

Product Images

Circular map for RR214781

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rnf146
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214781 representing NM_001012060
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAATGATGGCCGGCTGTGGTGAAATTGATCACTCACTAAATATGCTTCCTACCAATAAGAAGGCGA
  GTGAGACCTGTTCTAACACTGCACCTTCTCTAACAGTTCCCGAGTGTGCCATTTGTCTACAAACATGTGT
  TCATCCAGTCAGTCTGCCCTGTAAGCATGTTTTCTGTTATCTGTGTGTAAAGGGCGCTTCATGGCTCGGG
  AAGCGATGTGCTCTTTGTCGGCAAGAGATTCCTGAGGATTTTCTTGACAAGCCAACCTTGTTGTCACCAG
  AAGAACTTAAGGCTGCAAGCAGAGGAAATGGTGAATATGTGTGGTATTATGAAGGAAGAAATGGATGGTG
  GCAGTATGATGAGCGCACCAGTCGGGAGCTAGAAGATGCTTTTTCCAAAGGTAAAAAGAACACGGAAATG
  TTAATTGCTGGATTTCTGTACGTTGCTGATCTTGAAAACATGGTTCAATATAGGAGAAATGAACATGGAC
  GTCGCAGAAAGATTAAAAGAGATATAATAGATATACCAAAGAAGGGAGTGGCTGGACTTCGGCTGGACTG
  TGACAGCAACACTGTAAATCTAGCCAGAGAGAGTTCTGCCGATGGTGCGGACAGTGGGTCAGCACACACT
  GGAGCTTCTGTGCAGCTTCCAGTGCCATCTTCTACAAGGCCTCTAACATCAGTCGATGGTCAGTTAACCA
  GCCCTGTAACACCGTCCCCTGATGCAGGCGCTTCTTTGGAAGACTCTTTTGCTCATTTACAACTCAGTGG
  AGACAGCATAGCTGAAAGGAGTCATAGAGGTGAAGGAGAAGAAGATCACGAATCACCATCTTCTGGTAGA
  GTACCAGATACGTCCACTGAAGAAACAGAGTCGGATGCCAGTAGTGATATTGAGGATGCCCCTGTGGTGG
  TTGCACAGCACTCCTTGACCCAACAGAGACTTTTGGTTTCAAGTGCAAATCAGACAGTAGCTGAACGGTC
  TGACCGACCAGTTGCAGGGGGTGGGACCATGAGTGTCAATGTCAGATCCAGAAGGCCTGACGGACAGTGC
  ACAGTGACAGAGGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214781 representing NM_001012060
  Red=Cloning site Green=Tags(s)
  
  MEMMAGCGEIDHSLNMLPTNKKASETCSNTAPSLTVPECAICLQTCVHPVSLPCKHVFCYLCVKGASWLG
  KRCALCRQEIPEDFLDKPTLLSPEELKAASRGNGEYVWYYEGRNGWWQYDERTSRELEDAFSKGKKNTEM
  LIAGFLYVADLENMVQYRRNEHGRRRKIKRDIIDIPKKGVAGLRLDCDSNTVNLARESSADGADSGSAHT
  GASVQLPVPSSTRPLTSVDGQLTSPVTPSPDAGASLEDSFAHLQLSGDSIAERSHRGEGEEDHESPSSGR
  VPDTSTEETESDASSDIEDAPVVVAQHSLTQQRLLVSSANQTVAERSDRPVAGGGTMSVNVRSRRPDGQC
  TVTEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012060
• ORF Size: 1065 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012060.2, NP_001012060.2
• RefSeq Size: 1889 bp
• RefSeq ORF: 1068 bp
• Locus ID: 308051
• UniProt ID: Q5XIK5
• Cytogenetics: 1p11
• MW: 38.6 kDa
• Gene Summary: E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation. Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation (By similarity).[UniProtKB/Swiss-Prot Function]