Wdr3 (NM_001107705) Rat Tagged ORF Clone

CAT#: RR214659

Wdr3 (Myc-DDK-tagged ORF) - Rat WD repeat domain 3 (Wdr3), (10 ug)

Product Images

Circular map for RR214659

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wdr3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214659 representing NM_001107705
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTTACCAAACAGTATCTCCGCTATGTGGCTAGTGCTGTCTTTGGCTTGATTGGCAGCCAAAAAG
  GCAACATTGTTTTCGTGACACTCCGAGGTGAAAAAGGACGCTACGTGGCAGTACCTGCCTGTGAACACGT
  TTTTATCTGGGACCTAAGGAAAGGCGAGAAGATCCTTATCCTTCAGGGGCTTAAACAAGAAGTCACTTGC
  TTGTGTCCCTCCCCGGATGGGCTGCATCTAGCTGTTGGCTATGAGGATGGAGCCATCCGAGTCTTCAGCC
  TCCTCAGTGGTGAGGGAAACATAACCTTCAATGGACACAGAGCAGCCGTCACCAGCTTAAAGTACGATCA
  GCTAGGAGGCAGACTGGCTTCCGGGTCTAAGGATACGGATGTCATTATTTGGGATGTGATCAATGAAAGT
  GGCCTCTACCGTCTAAAGGGACACAAGGATGCCGTCACACAAGCTCTGTTTCTCCGAGAAAGGAATCTGC
  TAGTTACAAGTGGGAAAGACACTATGGTGAAGTGGTGGGACCTGGATAACCAGCACTGCTTCAAAACAAT
  GGTTGGCCACCGAACTGAGGTGTGGGGCTTGGTTCTGGTTTCAGAAGAAACTCGACTAATCACTGGGGCT
  GCAGACAGTGAGCTGAGAGCCTGGGACATCGCCTATCTACAGGAGATTGATGACCCAGAAGAACCAGAGC
  CCAAGAAAATCAAAGAGTGTCCAGGGATACAGGACACGCCTGAAGCAGAGGACCGCACCCTTGAGGCGGA
  TGATGAGAAGTCTGAAGACCGCATACTCTCATGTAGAAAAGCTGGCTCCTTAATGCGAGAAGGAAGAGAC
  AGAGTTGTGAACCTTGCCGTGGACAAGACAGGCCGGATCCTTGCTTGCCACGGAACTGATTCTGTGTTAG
  AAGTATTTTGTATTCTTTCCAAAGCAGAAGTTCAGAAGAAAATGGATAAAAAGCTAAAGAAAGCTAGAAA
  GAAAGCAAGATTGAACTCTGCCAATGAGGAGGAGGACCCTGAAACAAGTGTCTCAATGACTCTGCAGGAT
  GAGATCCTTCGGGTAACCAAGATCAAGACTTCTGCCAAGATCAAATCTTTTGACCTGATTCATTCGCCTC
  AAGGAGAGTTAAAGGCAGTCTTCCTGCTGCAGAACAATCTGGTAGAACTGTATTCGCTCAGTACATCCTT
  GCCAAACCCCCAGCCTGTGAGGACGAGCAGGATCACCATCGGAGGCCACCGCAGCGATGTGCGCACGCTG
  TCTTTCAGCTCGGATAACATAGCTGTGCTTTCAGCCGCAGCCGAGTCCGTTAAGATATGGAACAGGTCTA
  CCCTGCAGTGCATCCGCACCATGCCTTGTGAATATGCTCTCTGCTCCTTCTTTGTTCCTGGTGACAGGCA
  GGTGGTCATCGGGACAAAGACAGGAAATATGCAACTTTATGACCTGGCCTCGGGCAATCTCTTGGAGACA
  ATAGCTGCCCATGATGGAGCTCTGTGGTCCATGTCTCTTTCTCCAGATCAGCGTGGCTTTGTGACAGGTG
  GTGCTGATAAAGCTGTCAAGTTCTGGGATTTTGAGTTGGTGACAGATAAAAATAGTACCCAGAAGAGACT
  CTCTGTGAAACAAACTAGAATCTTGCAGCTAGATGAGGATGTCCTATATGTCTGCTACTCTCCTGATCAA
  AGGCTGCTGGCTGTATCTTTGCTGGACTGCACTGTGAAGATTTTCTATGTTGACACTTTAAAGTTTTTTC
  TCTCACTGTATGGACACAAGCTGCCTGTTATATGCATGGACATCTCCCATGATGGAGCACTAATAGCAAC
  TGGTTCTGCTGATAGAAATGTGAAAATCTGGGGTCTGGACTTTGGGGACTGCCACAGGTCTCTCTTTGCA
  CATGATGACAGTGTGATGCATCTCCGATTCGTGCCCAAGTCCCACCTTTTCTTCACCGCGGGGAAGGACC
  ACAAGATCAAGCAGTGGGATGCGGACAAGTTTGAGCACATCCAAACGCTGGAGGGGCATCACCAGGAAAT
  ATGGTGTTTGGCTGTCAGCCCTAGTGGAGACTACGTGGTGTCAGCCTCTCATGACAAGTCTCTGAGACTC
  TGGGAGAGAACGAGAGAGCCTCTTATTCTTGAGGAAGAAAGGGAACTGCAAAGGGAAGCAGAGTATGAGG
  AGAGTGTGGCCAAAGAAGAGCAGCCAGCAGTTCCAGGGGAGACCCAGGGTGACAACTACTTCACTGGGAA
  GAAGACAATTGAAACAGTCAAAGCGGCTGAGAGGATCATGGAGGCAATTGAGCTGTACCGAGAAGAAACT
  GCAAAAATGAAAGAACACAGAGCCATTTGTAGAGCAGCGGGGAAAGAGGTTCCTCTTCCTGTCAACCCCA
  TCCTGATGGCCCATGGCAATATTTCTCCCTCAGCGTATGTGTTAGAGACTTTTAAAGGGATCAGGTCAAG
  TGAACTGGAAGAGGCCCTGCTTGTGCTGCCGTTCTCCTATGTCCCAGACATTCTTACGCTGTTCAATGGG
  TTCATCCAGACAGGATCAGATGTCGAGCTGTTGTGCCGGTGCCTCTTTTTTCTCCTCAGGATTCACTTTG
  GCCAGATCACCAGCAACCAGATGCTTGTGCCCGTGATAGAGAAGCTAAAAGAAACAACTATTTCAAAAGT
  CAGACAAGTCCAGGATGTCATTGGCTTCAATATGGCTGGTCTCAATTATCTTAAGAGAGAATGCGAAGCA
  AAGAGTGAAGTTATGTTTTTTGCAGAAGCTACTAGTCACTTGGAAGAGAAGAAGAGAAAACGAAAAAATA
  GAAGAAAGATGATTTTAACACTGACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214659 representing NM_001107705
  Red=Cloning site Green=Tags(s)
  
  MGLTKQYLRYVASAVFGLIGSQKGNIVFVTLRGEKGRYVAVPACEHVFIWDLRKGEKILILQGLKQEVTC
  LCPSPDGLHLAVGYEDGAIRVFSLLSGEGNITFNGHRAAVTSLKYDQLGGRLASGSKDTDVIIWDVINES
  GLYRLKGHKDAVTQALFLRERNLLVTSGKDTMVKWWDLDNQHCFKTMVGHRTEVWGLVLVSEETRLITGA
  ADSELRAWDIAYLQEIDDPEEPEPKKIKECPGIQDTPEAEDRTLEADDEKSEDRILSCRKAGSLMREGRD
  RVVNLAVDKTGRILACHGTDSVLEVFCILSKAEVQKKMDKKLKKARKKARLNSANEEEDPETSVSMTLQD
  EILRVTKIKTSAKIKSFDLIHSPQGELKAVFLLQNNLVELYSLSTSLPNPQPVRTSRITIGGHRSDVRTL
  SFSSDNIAVLSAAAESVKIWNRSTLQCIRTMPCEYALCSFFVPGDRQVVIGTKTGNMQLYDLASGNLLET
  IAAHDGALWSMSLSPDQRGFVTGGADKAVKFWDFELVTDKNSTQKRLSVKQTRILQLDEDVLYVCYSPDQ
  RLLAVSLLDCTVKIFYVDTLKFFLSLYGHKLPVICMDISHDGALIATGSADRNVKIWGLDFGDCHRSLFA
  HDDSVMHLRFVPKSHLFFTAGKDHKIKQWDADKFEHIQTLEGHHQEIWCLAVSPSGDYVVSASHDKSLRL
  WERTREPLILEEERELQREAEYEESVAKEEQPAVPGETQGDNYFTGKKTIETVKAAERIMEAIELYREET
  AKMKEHRAICRAAGKEVPLPVNPILMAHGNISPSAYVLETFKGIRSSELEEALLVLPFSYVPDILTLFNG
  FIQTGSDVELLCRCLFFLLRIHFGQITSNQMLVPVIEKLKETTISKVRQVQDVIGFNMAGLNYLKRECEA
  KSEVMFFAEATSHLEEKKRKRKNRRKMILTLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107705
• ORF Size: 2826 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107705.1, NP_001101175.1
• RefSeq Size: 3417 bp
• RefSeq ORF: 2829 bp
• Locus ID: 310720
• Cytogenetics: 2q34
• MW: 106.1 kDa