Pp2d1 (NM_001134567) Rat Tagged ORF Clone

CAT#: RR214610

Pp2d1 (Myc-DDK-tagged ORF) - Rat similar to hypothetical protein phosphatase 2C domain containing protein (RGD1564811), (10 ug)

Product Images

Circular map for RR214610

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pp2d1
• Synonyms: RGD1564811
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214610 representing NM_001134567
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTTGAAATATATAGCCCTGTAAGAGTTTTTTTGAAATCAAAACAATGGGATGAGAAAACTTCAA
  AATTTACTTCAGATGAGGCTCTCCCAACTGTCAGAACAAATGTCAGAAGGCGTAGTAGACTGCCACGAAT
  GAATGACCCAGAGGAGTCCACTGACCAGCTGACTTCGGTTGACATGGGTGACATGGTCACATTCCCCTGC
  TCTGTCTGCCACCAAGAACTGAACACAGCTGGGAATTTTCTCCATAAGAAACATCACAATGCTCTGAGTA
  CGCTGGGTTTCGAGTGGATGGGAGGGAGGAAACCAAAGCCCAAAATGGTCAGTTTTCACAGAGAGTGTAT
  AATTTCTAACCTTTTGAGATCTTCTACATACAGTGAAAAAGTCCTTCAGAGTATGAATTATGCATTTGAG
  CTTCTTAGGAAGAAACAAATTCCGTCGTACTTCAAACTTTGTGACAAGGTCGGAGAGACTTCTATATATT
  CTCCAACCAGCTACCACCTTCTGATTAAAGGTATAGCCATCTGTAGCAATAGTAATTCAACCTGGAAAGC
  TGAGCCCAACTGTAAATTCACGGTCGTGAACGACTTTGGCGATAAAGCCAATGTGTGCTTTTTCGGTTTA
  TTTGATAGTCATCATGGTTACGCAGCAGCAGACCTGGCATCAAAGGAGTTTCAGGTTTTACTTCTCCATC
  AACTGTCTGTACAGGATCCTTCCTACCAGATGACAGCCGAGCAACAGGATCTCATCAATTCCTTTCACAC
  GGTGTTCAGGGAAGAATACAGAGCCAGAGAAGAGGCCTTCTCTTCCACATATAAAACCTTCAGAACCAAT
  AAACGGGAGTATGAGGATGTACACAAAGCGTTTGCCAAGGCATTCTGGAGAATGGATAGGCTTCTACGGC
  TGGGAAGGAACGAGGTCTCCCGGGTTCGGTGGAGCGGCTGTTCAGCACTCACGTGTATATTAGAGGGTGG
  GATCAAGAACCCACAGGCTACTAAGGACTGGGAAAAAACCTATCAGCATGGTGTTAACAGCTCTCCCTTC
  CAGAAGATTCCCCAGATCATCTCTGGAGTACTACACATTGCAAATGCTGGAAATGTACAAGCAGTCTTAT
  GCAGAAATGGAAAAGGATTTTGCCTGACCAAGGAGCACACTACACGAAACACTAAAGAAAGAAGAAGAGT
  ACTTTACAGCGAAGTAGGGATCAGTTCAGATGACCCCTATGGTCTCCTGGATGGGCACATAAAAACCACC
  AGGGGTCTTGGATTCCATGGAAACCTCAGACTAAAAAAGGCCATTATTCCAGTACCACAAACAATTTCTG
  TCCCTATAGATGATTTATGTCAGTTTCTCATCTTAGCCACCAATGGACTTTGGCAAGTTTTGGACAAGAA
  GGAAGTCACTGCACTGGTAATCACTTTGTTTCACGCATATAAAGAAACATGTGTTTCTGGCCCTAGAAAT
  AAACCCTGGCCATCTAAAAGCCTGCTTTTCCCACCAGACAGTAACATCCGCGTGCTGTTCCAATACCAGC
  CTGAAAATGAAGACATTACGTCAACTACAGATGTAATGAAAAGGTTATCTGACTCACCATTTGCTGACAC
  CAACATTCATCAAGATACATCTGCAGAAACATTTCTACCAAAAGCGACCTCTTATGACCCTTATAGCACA
  AAAGAGAGTAATAGCTTACCCACTACGAACAGTAAGCAGGAAAGTGAGAAAGAAATATGTATTAAGAACT
  TCTATAAAGGTGCAGCTGAGTACATTGGCTGTCAACTGGTAAGTGCTGCAATAGAAGGTGGTTCCAGAGA
  CAGCATCACTGTTATGGTAATGTTTCTCAATGGAAGTGAGTATCACAGGCGGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214610 representing NM_001134567
  Red=Cloning site Green=Tags(s)
  
  MDFEIYSPVRVFLKSKQWDEKTSKFTSDEALPTVRTNVRRRSRLPRMNDPEESTDQLTSVDMGDMVTFPC
  SVCHQELNTAGNFLHKKHHNALSTLGFEWMGGRKPKPKMVSFHRECIISNLLRSSTYSEKVLQSMNYAFE
  LLRKKQIPSYFKLCDKVGETSIYSPTSYHLLIKGIAICSNSNSTWKAEPNCKFTVVNDFGDKANVCFFGL
  FDSHHGYAAADLASKEFQVLLLHQLSVQDPSYQMTAEQQDLINSFHTVFREEYRAREEAFSSTYKTFRTN
  KREYEDVHKAFAKAFWRMDRLLRLGRNEVSRVRWSGCSALTCILEGGIKNPQATKDWEKTYQHGVNSSPF
  QKIPQIISGVLHIANAGNVQAVLCRNGKGFCLTKEHTTRNTKERRRVLYSEVGISSDDPYGLLDGHIKTT
  RGLGFHGNLRLKKAIIPVPQTISVPIDDLCQFLILATNGLWQVLDKKEVTALVITLFHAYKETCVSGPRN
  KPWPSKSLLFPPDSNIRVLFQYQPENEDITSTTDVMKRLSDSPFADTNIHQDTSAETFLPKATSYDPYST
  KESNSLPTTNSKQESEKEICIKNFYKGAAEYIGCQLVSAAIEGGSRDSITVMVMFLNGSEYHRRT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134567
• ORF Size: 1875 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134567.1, NP_001128039.1
• RefSeq Size: 2461 bp
• RefSeq ORF: 1878 bp
• Locus ID: 316157
• Cytogenetics: 14q21
• MW: 71.1 kDa