Dars2 (NM_001034143) Rat Tagged ORF Clone

CAT#: RR214584

Dars2 (Myc-DDK-tagged ORF) - Rat aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR214584

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dars2
• Synonyms: RGD1308286
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214584 representing NM_001034143
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATCTTGGTTCTTGGTTAAATCGTCTAGGCAGGGGTTTATCCAGACCCATCGGAAAGACCAAGCAGC
  CAATCTGGGGTTCTCTTTCCAGAAGTCTGACGTTGAGTTCACAGAGAGTTCCAGAATTCAGTAGCTTTGT
  TGCTCGGACCAACACATGTGGAGAACTTCGTTCTTCCCACTTAGGCCAAGAAGTCACTCTGTGTGGATGG
  ATTCAGTACCGAAGGCAAAACACCTTCTTAGTCCTAAGAGATTGCCACGGTCTCGTTCAAATCCTCATTC
  CCCAGGATGAGTCGGCTGCATCTGTGAGGAGGACTTTGTGTGAAGCCCCTGTGGAGTCTGTGGTGAGAGT
  ATCTGGGACGGTCATCGCCCGACCCCTGGGACAGGAGAATCCGAAAATGCCAACAGGTGAGATTGAAATC
  AAAGCTAAAACAGCTGAGCTCCTGAATGCCTGCAAAAAGCTGCCCTTTGAAATTAAAGACTTTGTGAAGA
  AGACGGAAGCCCTTCGTCTGCAGTATCGGTACTTAGACCTGCGCAGCTCCCAAATGCAGCACAACCTGCG
  GCTCAGGTCACAGATGGTCATGAAAATGCGGGAATATCTCTGTACTCTGCACGGATTTGTGGATATAGAA
  ACCCCAACATTGTTTAAGAGGACTCCTGGAGGTGCCAAGGAGTTTTTAGTACCGTCCAGGGAACCTGGAA
  GGTTTTATTCTCTCCCTCAGAGTCCTCAACAGTTTAAGCAGCTTCTGATGGTTGGTGGTTTAGACAGGTA
  CTTTCAGGTTGCCCGGTGTTATCGAGATGAAGGCTCAAGACCGGATAGGCAGCCAGAGTTTACTCAGATT
  GACATAGAGATGTCCTTTGTAGACCAGACTGGCATTCAGCATTTAGTTGAGGGTTTGCTGCATTATTCCT
  GGCCTGAAGATAAAGACCCTCTAGTGGCTCCTTTTCCTTCCATGACTTTTGCTGAGGCATTGGCTACCTA
  CGGAACTGATAAGCCAGATACTCGCTTTGGGATGAAGATTGTGGATATCAGTGATGTGTTCAGAAACACA
  GAGATCAGGTTTCTTCAGGATGCACTGGCTAAGCCCCAAGGAACTGTCAAAGCAATATGTGTTCATGAAG
  GAGCAAAGTACTTAAGGAAAGAAGACATTGAGTTCATTCGAAAGTTTGCAGCTCACCATTTCAGTCAGGA
  AGTCCTTCCTATATTCCTGAATGCCAGGAAGAACTGGAGTTCACCATTTGCTAAATTCATAACGGAGGAG
  GAAAGATTGGAGTTAACCAGATTGATGGAGATACAAGAAGACGACATGGTGCTGCTGACTGCTGGGCAAC
  ATGAGAAAGCATGCTCTTTGTTGGGGAAGTTACGACTAGAGTGTGCAGACCTTTTGGAAACGAGAGGACT
  GGCGCTCAGGGACCCAGCATTGTTCTCTTTTCTCTGGGTACTTGACTTCCCACTCTTCCTCGCCAAGGAA
  GAGAGTCCCACAGAGCTGGAGTCAGCCCACCACCCATTTACTGCTCCTCACCCCGGCGACATTCACCTCC
  TCTACACTGAGCCGGAAAAGGTTCGTGGCCAACACTACGACTTGGTTTTAAATGGCAATGAGATAGGAGG
  AGGCTCTATCCGCATTCATGATGCACAGCTACAGAGGTACATCCTGGAAACATTGCTAAAGGAAGATGTG
  AAATTGCTTTCCCATCTGCTTCAGGCTTTAGACTATGGGGCACCCCCACATGGAGGAATTGCCTTAGGGT
  TAGACAGACTGGTGTGTCTTGTCACGGGAGCACCCAGCATCAGAGATGTCATAGCCTTCCCTAAATCCTA
  CCGAGGACATGACCTTATGAGCAATGCTCCAGACACTGTGTCTCCTGAAGATCTGAAGCCTTACCATATC
  CACGTCTCATGGCCAACAGACTCAGAAGAGAGAGCATCCGCCACACCTTCCAAATACTTGAGCTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214584 representing NM_001034143
  Red=Cloning site Green=Tags(s)
  
  MYLGSWLNRLGRGLSRPIGKTKQPIWGSLSRSLTLSSQRVPEFSSFVARTNTCGELRSSHLGQEVTLCGW
  IQYRRQNTFLVLRDCHGLVQILIPQDESAASVRRTLCEAPVESVVRVSGTVIARPLGQENPKMPTGEIEI
  KAKTAELLNACKKLPFEIKDFVKKTEALRLQYRYLDLRSSQMQHNLRLRSQMVMKMREYLCTLHGFVDIE
  TPTLFKRTPGGAKEFLVPSREPGRFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQI
  DIEMSFVDQTGIQHLVEGLLHYSWPEDKDPLVAPFPSMTFAEALATYGTDKPDTRFGMKIVDISDVFRNT
  EIRFLQDALAKPQGTVKAICVHEGAKYLRKEDIEFIRKFAAHHFSQEVLPIFLNARKNWSSPFAKFITEE
  ERLELTRLMEIQEDDMVLLTAGQHEKACSLLGKLRLECADLLETRGLALRDPALFSFLWVLDFPLFLAKE
  ESPTELESAHHPFTAPHPGDIHLLYTEPEKVRGQHYDLVLNGNEIGGGSIRIHDAQLQRYILETLLKEDV
  KLLSHLLQALDYGAPPHGGIALGLDRLVCLVTGAPSIRDVIAFPKSYRGHDLMSNAPDTVSPEDLKPYHI
  HVSWPTDSEERASATPSKYLSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034143
• ORF Size: 1956 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034143.1, NP_001029315.1
• RefSeq Size: 2321 bp
• RefSeq ORF: 1959 bp
• Locus ID: 304919
• UniProt ID: Q3KRD0
• Cytogenetics: 13q22
• MW: 74 kDa