Clec4f (NM_053753) Rat Tagged ORF Clone

CAT#: RR214374

Clec4f (Myc-DDK-tagged ORF) - Rat C-type lectin domain family 4, member f (Clec4f), (10 ug)

Product Images

Circular map for RR214374

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Clec4f
• Synonyms: Clecsf13; Kclr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214374 representing NM_053753
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAGGCGGAACTGAACAGAGATGTGGCTAAATTCTGCACTGACAACCAGTGTGTCATCCTACAGC
  CCCAAGGACTGGGGCCTAAGTCAGCTGCTCCCATGGCTCCCAGGACACTGAGGCACGTCCAGGCCATTGT
  GGCACTAGTGGTGGTGACTGTGTTCTTCTCTCTCCTTGCTCTTTTTGTGGTGGTTTTACAGCCTTGGAGA
  CAGAAGCAGAATGAGGATCATCCTGTCAAAGCCGGGCTTCATGGAGGCAACTACTCTGGATCTGACAATT
  GTTCCCAATTTGTCCGGAGGGCAGAAATGCAAGAGGCTATCCAGAGTCTCAGGGCCTCTGGGAATTCTAG
  CAGCTGTCACAAGGAGATACAGACCCTGAAGTACCAGATGGACAATGTTAGTTCTCAGGTGCAGTTGCTT
  GGGGGCCATCTGGAGGAAGCCAATGCTGACATCCAGCAGGCAAAGGATGTGCTGAAGGGCACTGGGGCCT
  TGGCCTCGGAGACCCAGGCACTGAGAAGCTCCTTGGAGGTCGCCAGTGCGGATATCCATAGTCTGAGAGG
  CGATTTGGAAAAGGCAAATGCCATGACTTCCCAGACCCAAGGTCTCTTAAAGAGCAGCACAGACAACACC
  AGTGCTGAGCTCCATGTGCTGGGCAGAGGCCTGGAGGAAGCCCAGTCTGAGATCCAGGCTTTGAGGGGAA
  GTCTGCAGAGTTCAAATGACCTCGGCTCCCGGACTCAGAACTTTCTCCAGCACAGTATGGACAACATTAG
  TGCTGAAATCCAGGCCATGAGAGATGGTATGCAAAGAGCTGGCGAGGAGATGACCTCATTAAAGAAAGAT
  CTAGAAACACTTACTGCTCAGATTCAAAATGCAAATGGCCACCTGGAGCAGACAGATACCCAGATCCAGG
  GGCTAAAAGCTCAGTTGAAAAGCACCAGTTCCTTGAACTCCCAGATTGAGGTGGTCAATGGCAAATTGAA
  AGACTCCAGCAGAGAGTTACAGACACTGAGAAGGGACCTGAGTGATGTCTCAGCTTTGAAGTCCAACGTC
  CAGATGTTACAGAGCAATCTTCAGAAGGCCAAGGCAGAGGTACAGAGTTTGAAAACAGGTCTGGAGGCTA
  CCAAAACTCTCGCTGCCAAGATTCAGGGACAACAGAGTGACCTGGAGGCCCTGCAGAAAGCTGTGGCTGC
  ACACACGCAGGGGCAGAAGACTCAGAATCAGGTTCTCCAGCTGATCATGCAGGACTGGAAGTACTTCAAT
  GGAAAGTTTTATTACTTTTCTCGTGACAAGAAGTCATGGCATGAGGCCGAGAATTTCTGTGTGTCCCAGG
  GAGCACACCTGGCTTCGGTGACCTCTCAGGAGGAGCAGGCATTCCTGGTACAGATCACAAATGCTGTGGA
  CCATTGGATTGGGCTCACTGATCAGGGCACAGAGGGCAACTGGCGCTGGGTGGATGGGACACCATTCGAT
  TATGTCCAGAGCAGAAGGTTTTGGAGGAAAGGCCAGCCTGACAACTGGAGACACGGAAACGGAGAGCGTG
  AAGACTGTGTTCATCTGCAGCGGATGTGGAACGACATGGCCTGTGGGACCGCCTACAACTGGGTGTGCAA
  GAAGTCTACAGATTGGTCTGTGGCCAGAACGGACCAGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214374 representing NM_053753
  Red=Cloning site Green=Tags(s)
  
  MKEAELNRDVAKFCTDNQCVILQPQGLGPKSAAPMAPRTLRHVQAIVALVVVTVFFSLLALFVVVLQPWR
  QKQNEDHPVKAGLHGGNYSGSDNCSQFVRRAEMQEAIQSLRASGNSSSCHKEIQTLKYQMDNVSSQVQLL
  GGHLEEANADIQQAKDVLKGTGALASETQALRSSLEVASADIHSLRGDLEKANAMTSQTQGLLKSSTDNT
  SAELHVLGRGLEEAQSEIQALRGSLQSSNDLGSRTQNFLQHSMDNISAEIQAMRDGMQRAGEEMTSLKKD
  LETLTAQIQNANGHLEQTDTQIQGLKAQLKSTSSLNSQIEVVNGKLKDSSRELQTLRRDLSDVSALKSNV
  QMLQSNLQKAKAEVQSLKTGLEATKTLAAKIQGQQSDLEALQKAVAAHTQGQKTQNQVLQLIMQDWKYFN
  GKFYYFSRDKKSWHEAENFCVSQGAHLASVTSQEEQAFLVQITNAVDHWIGLTDQGTEGNWRWVDGTPFD
  YVQSRRFWRKGQPDNWRHGNGEREDCVHLQRMWNDMACGTAYNWVCKKSTDWSVARTDQS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053753
• ORF Size: 1650 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053753.1, NP_446205.1
• RefSeq Size: 1888 bp
• RefSeq ORF: 1653 bp
• Locus ID: 114598
• UniProt ID: P10716
• Cytogenetics: 4q34
• MW: 61.1 kDa
• Gene Summary: carbohydrate binding receptor with an affinity for fucose and galactose [RGD, Feb 2006]