Pcdh18 (NM_001100524) Rat Tagged ORF Clone

CAT#: RR214307

Pcdh18 (Myc-DDK-tagged ORF) - Rat protocadherin 18 (Pcdh18), (10 ug)

Product Images

Circular map for RR214307

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pcdh18
• Synonyms: MGC188762
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214307 representing NM_001100524
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCAAATAAATACTAAAATGCACTTTAGATTCGCACTTTCACTTCTGATGGCGTTTTTCAGCCACG
  ATGTCCTGGGTAAGAATCTGAAATACAGGATTTATGAGGAGCAGAGGGTCGGATCGGTAATTGCTAGACT
  ATCAGAAGATGTGGCTGATGTTTTACTGAAGCTACCAAATCCTTCTGCCATTCGTTTTCGAGCCATGCCA
  CGGGGGAATTCTCCTCTCCTGGTCGTAAATGAGAATACCGGAGAAATCAGCATAGGGGCTAAAATCGACC
  GCGAGCAACTATGCCAAAAGAACTTGAACTGTTCGATAGAGTTTGATGTGCTCACTCTGCCGACCGAGCA
  TCTGCAGCTGTTCCATATTGAAGTGGACGTGTTGGACATTAATGACAATTCCCCTCAATTCTCGAGACCC
  GTCATTCCCATTGAGATATCGGAGAGCGCAGCAGTTGGGACTCGCATCCCTCTGGACAGTGCGTTCGACC
  CCGATGTTGGGGAAAATTCCCTCCACACCTACTCGCTCTCTGCTAATGATTATTTTAATATCGAAGTGCG
  GACCAGGACGGACGGGGCCAAGTATGCTGAACTCATAGTGGTGAAAGAGTTGGATCGGGAGCTGAAGGCT
  AGCTATGAGCTGCAGCTCACGGCCTCTGACATGGGAGTGCCTCAGAGGTCTGGGTCCTCCATCCTGAAAA
  TAAGCATTTCGGATTCCAACGACAACAGCCCGGCCTTTGAGCAGCCCTCTTACACAATACAACTCTTAGA
  AAACTCCCCAGTTGGAACCTTGCTCCTCGATCTAAATGCCACTGATCCTGATGAGGGCGTTAATGGGAGA
  ATTGTATATTCTTTCAGCAGCCATGTGTCTCCCAAAATTATAGAGACTTTTAGGATCGACTCAGAAAGAG
  GACACTTGACTCTTTTCAAGCCGGTGGATTATGAAATCACCAAGTCCTATGAGATAGATGTTCAAGCCCA
  AGATCTGGGTCCCAATTCCATTCCTGCTCATTGCAAAATTATAATTAAGGTCGTGGATGTCAATGACAAT
  AAGCCTGAAATTAGCATAAACCTCATGTCCCCTGGAAAAGAAGAAGTGTCTTATGTTTTTGAAGGGGATC
  CCATTGATACATTTGTTGCTATCGTCAGGGTTCAAGACAAGGATTCTGGGCTGAATGGGGAAATAATCTG
  TAAGCTTCATGGGCATGGGCACTTTAAACTTCAGAAGACTTATGAAAACAACTACTTAATCTTGACCAAT
  GCCACTCTGGATAGAGAAAAGAGATCTGAGTACAGTTTGACTGTGATTGCTGAGGACAAGGGAACACCCA
  ACCTCTCTTCAGTGAGACATTTTACTGTTCAAGTCAATGACATAAATGACAATCCACCTCGCTTCCAGAG
  GAGCCAATATGAATTTGTCATCTCAGAGAATAATTCGCCAGGGGCGTACATCACCACAGTTACAGCCACC
  GATCCAGACCTGGGTGAAAATGGGCATGTGACATACACCATTTTGGAGAGTTTTGTCTTGGGAAGTTCCA
  TTACCACGTATGTAACCATTGATCCATCCAATGGCGCCATCTATGCCCTCAGGATCTTTGATCATGAGGA
  AGTGAGTCAGATCACTTTTGTGGTGGAAGCAAGGGATGGAGGGAGTCAGAAGCAACTCTCAAGCAACACC
  ACAGTTGTGCTGACCATCATTGACGAGAATGACAATGTCCCTGTGGTTGTAGGGCCTGCAATGCACAACA
  ACACTGCAGACATCTCCATCCCCAAAGGAGTCGAAAGTGGTTTCCATGTCACAAGACTAAGGGTAGTGGA
  CAGAGACTCTGGTGCAAATGCTGAATTCACCTGCTCCATAGTAGCTGGTAATGAGGAAAACATCTTTATC
  ATAGATCCAAGGTCATGTGACATCCATACCAACGTCAGCATAGACTCCATTTCCTCCACCGAATGGGCAC
  TTTCAGTTATCATCCAGGACAAGGGCAATCCTCCTCTGCACACCAAAGTCCTCCTGAGGTGCATGGTCTT
  TGACTATACAGAATCTGTGACAAGCACAGCCATGACTTCTGTCAGTCACGCATCCTTGGATGTGTCTATG
  ATCATAATTATTTCCTTAGGGGCAATCTGTGCAGTGTTGCTGGTTATTATGGTACTGTTCGCCACGAGGT
  GCAATAGAGAAAAGAAAGACACCAGGTCCTACAACTGCAGGGTGGCAGAATCCACTTACCAGCATCATCC
  CAAAAGGCCGTCCAGGCAGATTCACAAAGGAGACATTACACTGGTGCCCACCATCAATGGTACTCTGCCC
  ATCAGATCTCACCACAGATCCTCTCCATCTTCATCTCCAACCTTGGAAAGGGGGCAGATGGGCAGCCGCC
  AGAGTCACAACAGTCACCAGTCACTCAACAGTTTGGTGACCATCTCCTCAAACCACGTGCCAGAGAATTT
  TTCATTAGAACTCACCCATGCCACTCCTGCTGTTGAGCAGGTCTCACAGCTTCTCTCCATGCTCCACCAG
  GGGCAATATCAGCCACGGCCAAGCTTTCGAGGAAACAAATATTCCAGGAGCTATAGATATGCCCTTCAAG
  ACATGGATAAATTTAGCTTGAAAGACAGTGGCCGTGGAGATAGTGAAGCAGGAGACAGTGATTATGATTT
  GGGGCGAGATTCTCCAATAGACAGGCTCCTGGGAGAAGGATTCAGTGACCTCTTTCTCACTGATGGAAGA
  ATTCCAGCAGCCATGAGGCTATGTACGGAGGAATGCAGAGTCCTGGGCCACTCTGACCAGTGCTGGATGC
  CTCCACTGCCCTCGCCATCCTCAGACTACAGAAGCAACATGTTCATCCCCGGAGAAGAGTTCCCGGCTCA
  ACCTCAACAACAGCATTCTCATCCGGGCCTTGATGATGACAGTCAGCCTGCCGAATCTGGGGAGAAAAAA
  AAGAGCTTTTCCACCTTTGGGAAGGATTCCCCTAGCGACGAGGACTCCGGGGACTCTAGCACATCATCTC
  TGCTATCAGAAATGAACAGTGTGTTCCAACGCCTCCTCCCCGCATCCCTAGATACCTATTCTGAATGCAG
  CGAAGTGGACCGGTCCAACTCCCTGGAACGTCGAAAGGGTCCAGCACAGGGCAAAACTGGGGGTTACCCA
  CAAGGGGTTGCAGCCTGGGCAGCCAGCACACATTTTCAGAACCCCACCAGCAGCTCTGGGACCCCTCTGG
  GGACTCACTCCAGTGTGCAGCCTTCTTCCAAGTGGTTGCCAGCTATGGAGGAGATCCCTGAAAACTACGA
  GGAAGATGATTTTGACAATGTGCTGAACCATCTTAGTGATGGGAAACACGAACTCATGGATGCCAGCGAG
  CTGGTAGCTGAGATCAACAAACTGCTTCAAGATGTCCGCCAGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214307 representing NM_001100524
  Red=Cloning site Green=Tags(s)
  
  MHQINTKMHFRFALSLLMAFFSHDVLGKNLKYRIYEEQRVGSVIARLSEDVADVLLKLPNPSAIRFRAMP
  RGNSPLLVVNENTGEISIGAKIDREQLCQKNLNCSIEFDVLTLPTEHLQLFHIEVDVLDINDNSPQFSRP
  VIPIEISESAAVGTRIPLDSAFDPDVGENSLHTYSLSANDYFNIEVRTRTDGAKYAELIVVKELDRELKA
  SYELQLTASDMGVPQRSGSSILKISISDSNDNSPAFEQPSYTIQLLENSPVGTLLLDLNATDPDEGVNGR
  IVYSFSSHVSPKIIETFRIDSERGHLTLFKPVDYEITKSYEIDVQAQDLGPNSIPAHCKIIIKVVDVNDN
  KPEISINLMSPGKEEVSYVFEGDPIDTFVAIVRVQDKDSGLNGEIICKLHGHGHFKLQKTYENNYLILTN
  ATLDREKRSEYSLTVIAEDKGTPNLSSVRHFTVQVNDINDNPPRFQRSQYEFVISENNSPGAYITTVTAT
  DPDLGENGHVTYTILESFVLGSSITTYVTIDPSNGAIYALRIFDHEEVSQITFVVEARDGGSQKQLSSNT
  TVVLTIIDENDNVPVVVGPAMHNNTADISIPKGVESGFHVTRLRVVDRDSGANAEFTCSIVAGNEENIFI
  IDPRSCDIHTNVSIDSISSTEWALSVIIQDKGNPPLHTKVLLRCMVFDYTESVTSTAMTSVSHASLDVSM
  IIIISLGAICAVLLVIMVLFATRCNREKKDTRSYNCRVAESTYQHHPKRPSRQIHKGDITLVPTINGTLP
  IRSHHRSSPSSSPTLERGQMGSRQSHNSHQSLNSLVTISSNHVPENFSLELTHATPAVEQVSQLLSMLHQ
  GQYQPRPSFRGNKYSRSYRYALQDMDKFSLKDSGRGDSEAGDSDYDLGRDSPIDRLLGEGFSDLFLTDGR
  IPAAMRLCTEECRVLGHSDQCWMPPLPSPSSDYRSNMFIPGEEFPAQPQQQHSHPGLDDDSQPAESGEKK
  KSFSTFGKDSPSDEDSGDSSTSSLLSEMNSVFQRLLPASLDTYSECSEVDRSNSLERRKGPAQGKTGGYP
  QGVAAWAASTHFQNPTSSSGTPLGTHSSVQPSSKWLPAMEEIPENYEEDDFDNVLNHLSDGKHELMDASE
  LVAEINKLLQDVRQS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100524
• ORF Size: 3405 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001100524.1, NP_001093994.1
• RefSeq Size: 4927 bp
• RefSeq ORF: 3408 bp
• Locus ID: 295027
• Cytogenetics: 2q26
• MW: 125.6 kDa