Cenpn (NM_001008366) Rat Tagged Lenti ORF Clone
CAT#: RR214066L3
- LentiORF®
Lenti ORF clone of Cenpn (Myc-DDK-tagged ORF) - Rat centromere protein N (Cenpn), (10 ug)
"NM_001008366" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Cenpn |
Synonyms | Da2-4; Da2-27; RGD1310953 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RR214066).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001008366 |
ORF Size | 1020 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001008366.1, NP_001008367.1 |
RefSeq Size | 1865 bp |
RefSeq ORF | 1023 bp |
Locus ID | 361416 |
UniProt ID | Q5U2W4 |
Cytogenetics | 19q12 |
Gene Summary | Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN214066 | Cenpn (untagged ORF) - Rat centromere protein N (Cenpn), (10 ug) |
USD 503.00 |
|
RR214066 | Cenpn (Myc-DDK-tagged ORF) - Rat centromere protein N (Cenpn), (10 ug) |
USD 503.00 |
|
RR214066L4 | Lenti ORF clone of Cenpn (mGFP-tagged ORF) - Rat centromere protein N (Cenpn), (10 ug) |
USD 803.00 |
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