Tgm4 (NM_022713) Rat Tagged ORF Clone

CAT#: RR213904

Tgm4 (Myc-DDK-tagged ORF) - Rat transglutaminase 4 (prostate) (Tgm4), (10 ug)

Product Images

Circular map for RR213904

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tgm4
• Synonyms: Dp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213904 representing NM_022713
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCCAGGAACATGCTTGTCGTTTACTCTGTGAACTTGGAGAAAAAGCTGAACGCTGCTGCCCACC
  ACACGATTGAGTACCAAACTCAGAAGCTCGTGCTCAGGCGTGGACAGATATTCTCCCTGAAGGTGATGCT
  AAACAGACCGCTCCAATCTCATGATGAACTGAAGCTGATATTCAATACTGGACACAACATGCCATTCTAC
  ACTGTGGAGCTTGATCCAATGACATCCTACCGTTCCAAGGGCTGGCAGGTGAAGATTGCTAAACAGTCTG
  GTGTAGAGGTTGTATTGAATGTCATCAGTGCTGCCGACGCCGTGGTGGGGAGGTACACGATGAACGTGAA
  CGAATTTGACGCTGGAGTGTTCTTCCTTCTCTTCAATCCGTGGTGCTCAGATGACAGTGTCTTCATGGCT
  AGTGAGGAAGACCGAGCAGAGTACGTCCTTAATGATACCGGTTACATGTACATGGGTTTTGCCAAACAGA
  TTAAAGAGAAGCCCTGGACCTTTGGTCAGTTTGAGAAGTACATCTTGAACTGCTGCTTCCGATTGTTGAC
  CCATTTGGAGCCCAAGGAAATGCAGAGCCCAGTGCTTGTGTCCAGGGCCATTTGTACTATGATGTGTGCT
  GCAAACAATTTTGGAGTGCTGGTGGGGAACTGGACAGGAGACTATAGCAATGGCACTGCCCCGTATGTGT
  GGGCCAGCAGTGTACCCATTCTCCAGCAGCACTACATCACCAGGATGCCTGTACGCTTTGGCCAGTGCTG
  GGTTTTCTCTGGAGTCCTGACCACAGCACTGAGAGCAGTGGGCATCCCAGCTCGCAGCGTGACTAACTTT
  GAGTCAGCCCACGATACAGAAAAGAACCTCAGAGTAGACATCTACCTGGATGAATCGGGCAAGACAATTC
  CACATCTGACCAAAGACTCTGTCTGGAATTTCCATGTGTGGACAGATGCCTGGATGAAAAGACAGGATCT
  ACCCCAGGGCCATGATGGTTGGCAGGTCCTGGATTCTACACCACAGGAAATCAGTGAAGGACAATTCCGC
  ATTGGACCTTCACCGGTGTCTGCTATCCGCCAGGGATTGGTCCAAATCATGTATGACACCACATTTGTGT
  TCACGGAGGTGAACGGTGACAAGTACATCTGGCTGGTGAAGCAGAACCAGGAAAGGGAGAAGAATGTCCT
  CATCGCTGTGGAGACTGCTAGCATCGGCAAGAACATCAGCACCAAGATGGTGGGCGAGAACAGGCGTCAA
  GATATTACCTTGCATTACAAGTTCCCAGAAGGCAGCCCAGAGGAGAGGAAGGCCATGGAGAAGGCCTCTG
  GGAAACGTCCTGATGACAAGCTCAACTCTCGGACACTTCACATATCTGTCCTCCAGAACTCTGTGGAGCT
  AGGACATCCTATCAATTTGACCATAGTGTTGAAAAGGAAAACGGCCACACCACAGAATGTCAACATTTCA
  TGCTCCCTTGACCTACAGACTTACACAGGCAACAAGAAGACAAACCTGGGAGTCATCCAGAAGACTGTGC
  AGATTCAAGGCCAAGAATCAGAGGTGTCTCTCAGCATGGATTCCAGTTTCTACATCTATAAACTGGGCAT
  GGTTGATGATGAGATGGTCATTAAAGGGTTCATCATTGCAGAAATTGTTGATTCTGGTGAACGGGTAGCC
  ACTGATACGACCCTGTGTTTCCTGTACTCTGCCTTCTCTGTAGAGATGCCGAGCACAAGCAAGGTCAACC
  AGCCTCTGACCATCACCTGCAACTTCAAGAACACCCTGCCCATCCCTTTGACTAACATCAAGTTCTCAGT
  GGAAAGCCTGGGCCTCAATAACATGAAGTCCTGGGAGCAAGAGACGGTGCCGCCCGGAAAGACCATCAAT
  TTCCAGATAGAATGCACCCCAGTGAAAACTGGACCCAGGAAGTTCATTGTCAAGTTTATTTCCAGACAAG
  TGAAAGAGGTTCATGCCGAGAAGGTCGTTCTCATCACAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213904 representing NM_022713
  Red=Cloning site Green=Tags(s)
  
  MDSRNMLVVYSVNLEKKLNAAAHHTIEYQTQKLVLRRGQIFSLKVMLNRPLQSHDELKLIFNTGHNMPFY
  TVELDPMTSYRSKGWQVKIAKQSGVEVVLNVISAADAVVGRYTMNVNEFDAGVFFLLFNPWCSDDSVFMA
  SEEDRAEYVLNDTGYMYMGFAKQIKEKPWTFGQFEKYILNCCFRLLTHLEPKEMQSPVLVSRAICTMMCA
  ANNFGVLVGNWTGDYSNGTAPYVWASSVPILQQHYITRMPVRFGQCWVFSGVLTTALRAVGIPARSVTNF
  ESAHDTEKNLRVDIYLDESGKTIPHLTKDSVWNFHVWTDAWMKRQDLPQGHDGWQVLDSTPQEISEGQFR
  IGPSPVSAIRQGLVQIMYDTTFVFTEVNGDKYIWLVKQNQEREKNVLIAVETASIGKNISTKMVGENRRQ
  DITLHYKFPEGSPEERKAMEKASGKRPDDKLNSRTLHISVLQNSVELGHPINLTIVLKRKTATPQNVNIS
  CSLDLQTYTGNKKTNLGVIQKTVQIQGQESEVSLSMDSSFYIYKLGMVDDEMVIKGFIIAEIVDSGERVA
  TDTTLCFLYSAFSVEMPSTSKVNQPLTITCNFKNTLPIPLTNIKFSVESLGLNNMKSWEQETVPPGKTIN
  FQIECTPVKTGPRKFIVKFISRQVKEVHAEKVVLITK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022713
• ORF Size: 2001 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022713.2, NP_073204.2
• RefSeq Size: 2868 bp
• RefSeq ORF: 2004 bp
• Locus ID: 64679
• UniProt ID: Q99041
• Cytogenetics: 8q32
• MW: 75.6 kDa
• Gene Summary: may act as an androgen-regulated transglutaminase [RGD, Feb 2006]