Tpcn1 (NM_139332) Rat Tagged ORF Clone

CAT#: RR213405

Tpcn1 (Myc-DDK-tagged ORF) - Rat two pore segment channel 1 (Tpcn1), (10 ug)

Product Images

Circular map for RR213405

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tpcn1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213405 representing NM_139332
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTAATTTTAGACGACGACGTGCTGCTCATCCTGACCTTGGACGAGGAGCTGAGTGCGCCGCTGA
  CTCCTTCCAACGGCCTGGGCCAAGAAGACCTGCCCAGCAAAAATGGGGGCGGCCAAAGCGGCCCCAACTC
  CCAAGTTCCCAGTCTGGTCTCTGGAGCCGACAGTCCCCCCTCCAGTCCCCCCGGACACAACTGGGAGATG
  AATTATCAAGAGGCAGCAATCTACCTCCAGGAAGGCCAGAACAATGACAAGTTCTTCACCCACCCCAAGG
  ATGCCAGAGCGCTGGCTGCCTACCTCTTCGTACACAACCACTTCTTTTATATGATGGAGCTGCTCACAGC
  CCTGCTTCTGCTGCTGCTGTCACTGTGCGAGTCACCCGCTGTCCCTGCGCTCAAGCTCCGAACTTACGTC
  CACGCCACCCTGGAGCTGTTCGCCCTCATGGTGGTGGTATTTGAACTCTGCATGAAATTGCGGTGGCTGG
  GCTTCCACACATTCGTCAGGCACAAACGCACCATGGTCAAGACGTCTGTTTTGGTGGTGCAGTTCATCGA
  GGCCATTGTGGTGCTGGTGCGGCAGACGTCCCACGTGCGGGTGACCCGGGCACTACGCTGCATTTTCCTG
  GTGGACTGTCGGTACTGTGGTGGCGTGCGGCGCAACCTGCGGCAGATCTTCCAGTCGCTGCCGCCTTTCA
  TGGACATCCTCCTGCTGCTGCTCTTCTTTATGATCATCTTCGCCATCCTCGGTTTCTACTTATTCTCTAC
  GAACCCTTCCGACCCTTACTTCAACACCCTGGAGAACAGCATCGTCAACCTGTTTGTTCTCCTGACCACA
  GCCAACTTTCCAGACGTCATGATGCCCTCCTACTCCCGGAACCCCTGGTCCTGTGTCTTCTTTATCGTGT
  ACCTCTCCATTGAGCTGTACTTCATCATGAACCTGCTCCTAGCCGTGGTGTTCGACACCTTCAACGACAT
  CGAAAAGCACAAGTTCAAGTCCTTGCTGCTGCATAAGCGAACAGCCATCCAGCACGCATACCACCTGCTC
  GTCAGCCAACGGAGGCCGGCCGGCATCTCCTACAGGCAGTTTGAAGGCTTGATGCGCTTCTACAAGCCCC
  GGATGAGCGCTAGGGAGCGCTTCCTGACTTTCAAGGCCCTGAATCAGAGCAACACACCTCTGCTTAGCCT
  GAAGGACTTCTATGATATTTATGAAGTTGCTGCTCTGCAGTGGAAGGCAAAGAAGAACAGACAGCACTGG
  TTTGACGAGCTTCCCCGGACAGCCTTCCTCATCTTCAAAGGGATTAACATCCTTGTGAATTCCAAGGCCT
  TCCAGTATTTCATGTACCTGGTGGTGGCTGTCAACGGTGTCTGGATCCTAGTGGAGACATTCATGTTGAA
  AGGTGGGAATTTCATCTCAAAGCACGTGCCCTGGAGTTACCTCGTGTTTCTTACCATCTATGGAGTTGAG
  CTGTTCATGAAGGTGGCTGGCCTGGGCCCTGTGGAGTACTTGTCCTCTGGATGGAACCTGTTCGATTTCT
  CAGTCACAGCATTTGCCTTCCTGGGACTGCTCGCGCTGACGCTCAACATGGAGCCCTTCTACTTTATTGT
  GGTCCTGCGTCCCCTTCAGCTGCTGAGGTTATTCAAGTTGAAGAAACGCTACCGCAATGTGTTGGACACC
  ATGTTTGAGCTGCTGCCACGAATGGCCAGCCTCGGCCTCACACTGCTCACCTTCTACTACTCCTTCGCCA
  TCGTGGGCATGGAGTTCTTCAGTGGGCGACTGTCCCCCAACTGCTGCAACTCCAGCACGGTGGCTGACGC
  CTATCGATTCATCAATCACACGGTGGGCAATAAGACCAAGGTGGAGGAAGGCTACTACTATCTCAATAAC
  TTTGACAACATCCTCAACAGCTTTGTGACCTTGTTTGAGCTCACCGTTGTCAACAACTGGTACATCATCA
  TGGAAGGCGTCACCTCGCAGACTTCCCACTGGAGCCGCCTGTACTTCATGACCTTTTACATCGTGACCAT
  GGTGGTGATGACCATTATCGTGGCCTTCATCCTGGAGGCCTTCGTCTTCCGCATGAACTACAGCCGCAAG
  AGCCAGGAATCGGAAGTGGACAGTGGCATCGTCATCGAGAAGGAGATGTCCAAGGAGGAGCTTCTGGCCA
  TCCTGGAGCTTCATCGAGAGGCGCGAGGCACCTCCTCTGACATCACCCGGCTGCTCGACACCCTCTCTCA
  GATGGAGAAATACCAGCAAAACTCCATGGTGTTTCTGGGACGGCGATCGAGAACCAAAAGTGACCTGAGC
  CTGAAGATGTACCAGGAGGAGATCCAGGAGTGGTACGAGGAGCATGCCCGGGAACAGGAGCAGCAGCAGC
  TCAGGGGCAGCGCGCCAAGCCCTGCAGCCCAGCAGACCCCCGGCAGTCGCCAGCGCTCCCAGACCGTCAC
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213405 representing NM_139332
  Red=Cloning site Green=Tags(s)
  
  MSVILDDDVLLILTLDEELSAPLTPSNGLGQEDLPSKNGGGQSGPNSQVPSLVSGADSPPSSPPGHNWEM
  NYQEAAIYLQEGQNNDKFFTHPKDARALAAYLFVHNHFFYMMELLTALLLLLLSLCESPAVPALKLRTYV
  HATLELFALMVVVFELCMKLRWLGFHTFVRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFL
  VDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSTNPSDPYFNTLENSIVNLFVLLTT
  ANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYHLL
  VSQRRPAGISYRQFEGLMRFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKAKKNRQHW
  FDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFISKHVPWSYLVFLTIYGVE
  LFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLALTLNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDT
  MFELLPRMASLGLTLLTFYYSFAIVGMEFFSGRLSPNCCNSSTVADAYRFINHTVGNKTKVEEGYYYLNN
  FDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRK
  SQESEVDSGIVIEKEMSKEELLAILELHREARGTSSDITRLLDTLSQMEKYQQNSMVFLGRRSRTKSDLS
  LKMYQEEIQEWYEEHAREQEQQQLRGSAPSPAAQQTPGSRQRSQTVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139332
• ORF Size: 2451 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139332.3, NP_647548.2
• RefSeq Size: 4735 bp
• RefSeq ORF: 2454 bp
• Locus ID: 246215
• UniProt ID: Q9WTN5
• Cytogenetics: 12q16
• MW: 94.4 kDa
• Gene Summary: two-pore voltage-gated sodium and calcium channel [RGD, Feb 2006]