Psen2 (NM_031087) Rat Tagged ORF Clone

CAT#: RR212700

Psen2 (Myc-DDK-tagged ORF) - Rat presenilin 2 (Psen2), (10 ug)

Product Images

Circular map for RR212700

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Psen2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212700 representing NM_031087
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCACATTCATGGCCTCTGATAGCGAGGAAGAGGTGTGCGATGAGCGGACGTCCTTGATGTCAGCCG
  AGAGCCCTACATCACGTTCCTGCCAGGACAGCAGGCCAGGCCCAGAGGATGGAGAGAACACTGCCCAGTG
  GAGGAGCCAGGAGAACGAGGACGACTGTGAGGAGGACCCGGACCACTATGCCTGCAGTGGGGTTCCTGGG
  CGACCATCGGGCCTGGAGGAGGAGCTGACCCTCAAGTATGGGGCGAAGCACGTGATCATGCTGTTCGTGC
  CTGTCACGCTGTGCATGATTGTGGTGGTGGCCACCATCAAGTCCGTGCGTTTCTACACGGAGAAGAACGG
  GCAGCTCATCTACACGCCCTTCACCGAGGACACACCCTCTGTGGGCCAGCGGCTCCTCAACTCCGTGCTG
  AACACTCTGATCATGATCAGCGTCATTGTGGTCATGACTATCTTCCTAGTGGTACTCTACAAATATCGCT
  GTTACAAGTTCATCCATGGCTGGCTGATCATGTCCTCCCTGATGCTCCTCTTCTTGTTCACCTACATCTA
  CCTCGGGGAAGTGTTCAAGACCTACAATGTGGCCATGGACTACCCCACACTGTTCCTGGCTGTCTGGAAC
  TTCGGGGCAGTGGGCATGGTGTGCATCCACTGGAAGGGTCCCCTGGTGCTCCAGCAGGCTTACCTCATTG
  TGATCAGCGCACTCATGGCCCTGGTGTTCATCAAGTACCTGCCGGAGTGGTCCGCTTGGGTCATCTTGGG
  CGCCATCTCTGTGTACGATCTCGTGGCTGTGCTGTGCCCCAAAGGGCCACTGAGGATGCTGGTGGAAACT
  GCCCAGGAAAGAAACGAGCCCATATTTCCTGCCCTCATATACTCATCTGCCATGGTGTGGACGGTAGGCA
  TGGCGAAGCTGGACCCCTCCTCTCAGGGAGCACTGCAGCTCCCTTACGATCCAGAGATGGAAGAAGACTC
  CTATGACAGTTTTGGGGAACCCTCATACCCTGAAGCCTTCGAAGCTCCCCAGCCTGGCTACCCAGGGGAA
  GAGCCGGAGGAGGAGGAGGAAAGGGGTGTAAAGCTTGGTCTGGGAGACTTCATCTTCTACAGCGTTCTGG
  TGGGCAAGGCAGCAGCCACAGGCAACGGAGACTGGAGCACGACGCTGGCCTGCTTTATCGCCATCCTCAT
  TGGCTTGTGTCTCACCCTCCTGCTGCTCGCCGTGTTCAAGAAGGCCCTGCCCGCCCTCCCCATCTCCATC
  ACCTTCGGGCTCATCTTTTACTTCTCCACAGACAACCTGGTGCGTCCTTTCATGGACACGTTGGCCTCCC
  ACCAGCTCTACATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212700 representing NM_031087
  Red=Cloning site Green=Tags(s)
  
  MLTFMASDSEEEVCDERTSLMSAESPTSRSCQDSRPGPEDGENTAQWRSQENEDDCEEDPDHYACSGVPG
  RPSGLEEELTLKYGAKHVIMLFVPVTLCMIVVVATIKSVRFYTEKNGQLIYTPFTEDTPSVGQRLLNSVL
  NTLIMISVIVVMTIFLVVLYKYRCYKFIHGWLIMSSLMLLFLFTYIYLGEVFKTYNVAMDYPTLFLAVWN
  FGAVGMVCIHWKGPLVLQQAYLIVISALMALVFIKYLPEWSAWVILGAISVYDLVAVLCPKGPLRMLVET
  AQERNEPIFPALIYSSAMVWTVGMAKLDPSSQGALQLPYDPEMEEDSYDSFGEPSYPEAFEAPQPGYPGE
  EPEEEEERGVKLGLGDFIFYSVLVGKAAATGNGDWSTTLACFIAILIGLCLTLLLLAVFKKALPALPISI
  TFGLIFYFSTDNLVRPFMDTLASHQLYI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031087
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031087.2, NP_112349.2
• RefSeq Size: 1628 bp
• RefSeq ORF: 1347 bp
• Locus ID: 81751
• UniProt ID: O88777
• Cytogenetics: 13q26
• MW: 50.1 kDa
• Gene Summary: mutations in human homolog responsible for chromosome 1-linked familial Alzheimer's disease; expression increased during brain development but decreased in the adult [RGD, Feb 2006]