Qars (NM_001007624) Rat Tagged ORF Clone

CAT#: RR212669

Qars (Myc-DDK-tagged ORF) - Rat glutaminyl-tRNA synthetase (Qars), (10 ug)

Product Images

Circular map for RR212669

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Qars
• Synonyms: GlnRS; RGD1562301
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212669 representing NM_001007624
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACTCCGGATTCGCTGGCGCTGTTCACCGGCCTTGGCCTTAGCGAAAACAAGGCCCGCGAGACTC
  TCAAGAACGCGGCTTTGAGCACTCAGCTGCGCGAGGCGGCGACCCAGGCACAGCAGACTCTAGGGTCTAC
  CATCGACAAGGCTACTGGGACCCTGCTATATGGCTTGGCCTCCCGACTCAGGGATACTCGGCGTCTTTCT
  TTCCTTGTGGGCTATATAGCCAATAAGAAGATCCACACTGAGCTCCAGCTGAGCGCTGCTCTTGAATATG
  TTCGGAGTCATCCCCTGGATCCCATTGATACCAAGGACTTCGAGCAGGAATGTGGTGTTGGTGTGGTGGT
  GACACCAGAGCAGATTGAGGAAGCTGTGGAGGCCACCATAAATAGGCATCGTCCACAGCTCTTAGTGGAA
  CGGTACCGGTTCAGCATGGGGCTGTTAATGGGAGAGGCTCGGGCTGCGCTCAGATGGGCAGATGGCAAAA
  TGATCAAGAACGAAGTGGATATGCAGGTCCTCCACCTGCTGGGGCCCAAAATGGAAGCTGATCTGGAGAA
  GAAGCCCAAGGTGGCAAAGGCACGGCTGGAAGAAACAGACAGGAAGACAGCAAAAGATGTGGTGGAGAAC
  GGTGAAGTGGCTGGCCAGACCCTGTCTCTAATGGAGCAGCTCCGGGGAGAGGCCCTGAAGTTTCATAAAC
  CAGGTGAGAACTATAAGACGCCAGGCTATGTGACCACGCCACATACCATGGATCTGCTAAAGCAGCACCT
  GGAGATCACTGGGGGACAGGTACGAACCCGGTTTCCCCCAGAGCCCAATGGAATCCTGCATATTGGACAT
  GCCAAAGCCATCAATTTCAACTTTGGTTATGCCAAGGCCAACAACGGTATTTGTTTTCTGCGTTTTGATG
  ACACCAACCCTGAGAAGGAAGAAGCGAAATTCTTTACTGCTATTTATGACATGGTGACCTGGCTGGGTTA
  CACACCTTACAAAGTAACATACGCTTCTGACTATTTTGACCAGTTGTATGCCTGGGCTGTGGAACTGATC
  CGTAGGGGTCAAGCTTATGTTTGTCATCAGAGAGGGGAAGAGCTCAAAGGCCATAACCCTTTACCTTCAC
  CATGGAGAGACCGGCCTATTGAGGAATCCTTGCTGCTCTTTGAGGCAATGCGAAAGGGCAAATTTGCAGA
  GGGTGAGGCCACGCTTCGAATGAAGTTGGTGATGGAAGATGGAAAGATGGACCCTGTGGCCTATCGAGTC
  AAGTATACGCCACACCATCGCACAGGGGATAAATGGTGCATCTACCCCACCTACGACTACACACACTGTC
  TCTGTGATTCCATCGAGCACATCACCCACTCACTGTGTACCAAGGAATTCCAGGCTCGACGGTCTTCCTA
  CTTTTGGTTGTGTAATGCACTGGATGTCTACTGCCCTGTCCAGTGGGAATATGGCCGCCTCAATCTGCAC
  TATGCTGTTGTCTCAAAGCGGAAGATTCTCCAGCTTGTGGCAGCTGGTGCTGTTCGGGACTGGGATGACC
  CACGGCTCTTCACACTTACTGCCCTACGACGACGGGGTTTTCCACCTGAGGCCATCAACAACTTCTGTGC
  TCGGGTGGGGGTCACAGTGGCACAGACCACAATGGAACCTCATCTTCTGGAAGCCTGTGTGCGTGACGTG
  CTGAATGACACAGCCCCGCGTGCCATGGCTGTGCTAGAGCCACTACAAGTTGTCATCACTAACTTTCCTG
  CTCCCAAGCCCTTGGACATCCGAGTGCCAAACTTCCCAGCTGATGAGACCAAGGGCTTCCACCAGGTTCC
  TTTTGCTTCCACTGTCTTCATTGAGAGAACGGACTTTAAGGAGGAGTCAGAACCAGGCTATAAGCGCCTA
  GCCTGGGGCCAGCCTGTGGGCCTGAGACACACTGGTTACGTCATTGAACTGCAGCATGTTGTCAGGGGCT
  CCAGTGGCTGTGTGGAATGCTTGGAGGTGACCTGTAGACGAGCTGATGCTGGAGAGAAGCCCAAGGCCTT
  TATTCACTGGGTGTCACAGCCTCTGGTGTGTGAGATTCGCCTCTATGAGCGACTATTCCAGCACAAGAAC
  CCTGAAGACCCTGTCGAAGTGCCTGGTGGATTCCTAAGTGACTTGAACCCGGCATCACTACAAGTGATTA
  AAGGAGCGTTGGTGGACTGCTCTGTGGCTTTGGCAAAGCCCTTTGACAAGTTCCAGTTTGAGCGCCTTGG
  GTACTTCTCTGTGGATCCGGATAGCCATCAAGGACAGGTTGTCTTCAACCGAACAGTCACACTCAAAGAA
  GACCCAGGCAAAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212669 representing NM_001007624
  Red=Cloning site Green=Tags(s)
  
  MATPDSLALFTGLGLSENKARETLKNAALSTQLREAATQAQQTLGSTIDKATGTLLYGLASRLRDTRRLS
  FLVGYIANKKIHTELQLSAALEYVRSHPLDPIDTKDFEQECGVGVVVTPEQIEEAVEATINRHRPQLLVE
  RYRFSMGLLMGEARAALRWADGKMIKNEVDMQVLHLLGPKMEADLEKKPKVAKARLEETDRKTAKDVVEN
  GEVAGQTLSLMEQLRGEALKFHKPGENYKTPGYVTTPHTMDLLKQHLEITGGQVRTRFPPEPNGILHIGH
  AKAINFNFGYAKANNGICFLRFDDTNPEKEEAKFFTAIYDMVTWLGYTPYKVTYASDYFDQLYAWAVELI
  RRGQAYVCHQRGEELKGHNPLPSPWRDRPIEESLLLFEAMRKGKFAEGEATLRMKLVMEDGKMDPVAYRV
  KYTPHHRTGDKWCIYPTYDYTHCLCDSIEHITHSLCTKEFQARRSSYFWLCNALDVYCPVQWEYGRLNLH
  YAVVSKRKILQLVAAGAVRDWDDPRLFTLTALRRRGFPPEAINNFCARVGVTVAQTTMEPHLLEACVRDV
  LNDTAPRAMAVLEPLQVVITNFPAPKPLDIRVPNFPADETKGFHQVPFASTVFIERTDFKEESEPGYKRL
  AWGQPVGLRHTGYVIELQHVVRGSSGCVECLEVTCRRADAGEKPKAFIHWVSQPLVCEIRLYERLFQHKN
  PEDPVEVPGGFLSDLNPASLQVIKGALVDCSVALAKPFDKFQFERLGYFSVDPDSHQGQVVFNRTVTLKE
  DPGKV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007624
• ORF Size: 2325 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007624.1, NP_001007625.1
• RefSeq Size: 2794 bp
• RefSeq ORF: 2328 bp
• Locus ID: 290868
• UniProt ID: Q66H61
• Cytogenetics: 8q32
• MW: 87.7 kDa
• Gene Summary: Glutamine--tRNA ligase. Plays a critical role in brain development.[UniProtKB/Swiss-Prot Function]